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Asperity models for ground motion prediction is widely used in Japan. Here we expand the application of these asperity models to predict fault displacement caused by surface rupture. The proposed approach is rather simple and practical for the use in fault displacement hazard analysis in nuclear installations and other critical infrastructures, as it is the emphasis of the current topical issue. The proposed method mainly consists of two steps. The first step consists in the characterization of asperities at the seismogenic zone based on the kinematic asperity source model following Irikura's recipe (Irikura and Miyake in Pure Applied Geophysics, 168:85–104, 2011) for strong ground motion prediction. Since the kinematic model does not take into account surface rupture mechanism, this first step assumes that the fault is buried, and then by trial and error procedure the stress drop on the asperities are estimated, so that the average slip at each asperity be consistent with the ones from the kinematic model. At this stage, the dynamic model predicts strong ground motion consistent with those from the kinematic model. In the second step, the surface rupture is included by calibrating the shallow layer (SL) with stress drop, strength excess and critical slip distance, so that the final fault displacement along the fault be consistent with observations. The 2010 Mw 7.0 Darfield (New Zealand) earthquake is used to test the proposed method. Surface-rupturing was observed in several sites along the main fault reaching values of fault displacement larger than 5 m. The main fault of this earthquake is strike-slip, almost vertical. Therefore, a simplified planar fault asperity model to capture the main features of the fault displacement is assumed. The fault dimensions are assumed to have a length of 60 km and a width of 24 km with three asperities. The preferred model of the first step predicts average slip for each asperity of 2.7, 2.7, and 2 m corresponding, respectively, to stress drops of 6.0 MPa, 8.5 MPa, and 7.0 MPa. In the second step, the surface rupture is calibrated assuming a SL zone of 3 km depth. We found that negative stress drop is not necessary in the SL, because this strongly inhibits surface rupturing. Our preferred model produces fault displacement distribution closer to the observed ones, but average slip at each asperity increases to 3.4 m, 3.2 and 2.8 m. This increase in average slip is due to the contribution of surface rupturing. Ground motion differences between fault-surface rupturing and buried models are negligible, except at the very near-source. These differences are attributed to the SL rupture that mainly affect the ground motion at the very near-source. Overall, our simple asperity model captures the main features of the observed fault displacement and near-source ground motion, proving that the proposed simple and practical two step-procedure provides meaningful estimate of fault displacement and near-source ground motion consistent with observations, as such, this method has the potential to be used in practical fault displacement hazard analysis. [ABSTRACT FROM AUTHOR]

This study investigates the relationship among three critical variables in economic communication - recession coverage, the state of the economy and consumer confidence - in Japan. These time-series variables are intricately linked with one another during the period of 1988-99, roughly the `Lost Decade'. However, the study discovered that the linkage between these variables in Japan differs from that found for the US during the recession in the early 1990s. Japanese newspaper coverage followed the economy and public sentiment at different time-lags. Additionally, the Japanese's confidence level, regardless of the economic condition, can be predicted by the economy indicator but not by the recession coverage. Contrary to the literature, the study finds little direct media impact on either the economy or consumer confidence - a phenomenon that could be accounted for by the Japanese's deep pessimism about recovery and the longer duration of the recession. [ABSTRACT FROM AUTHOR]

Background: Esophageal squamous cell carcinoma (ESCC) has a poor prognosis, with limited second-line systemic therapy options, and represents an increasing disease burden in Japan. In the phase 3 RATIONALE-302 study, the anti-programmed cell death protein 1 antibody, tislelizumab, significantly improved overall survival (OS) versus chemotherapy as second-line treatment for advanced/metastatic ESCC. Here, we report the Japanese patient subgroup results., Methods: Patients with advanced/metastatic ESCC, with disease progression during/after first-line systemic therapy were randomized 1:1 to open-label tislelizumab 200 mg every 3 weeks or investigator's choice of chemotherapy (paclitaxel/docetaxel). Efficacy and safety were assessed in all randomized Japanese patients., Results: The Japanese subgroup comprised 50 patients (n = 25 per arm). Tislelizumab improved OS versus chemotherapy (median: 9.8 vs. 7.6 months; HR 0.59; 95% CI 0.31, 1.12). Among patients with programmed death-ligand 1 score ≥ 10%, median OS was 12.5 months with tislelizumab (n = 10) versus 2.9 months with chemotherapy (n = 6) (HR 0.31; 95% CI 0.09, 1.03). Tislelizumab improved progression-free survival versus chemotherapy (median: 3.6 vs. 1.7 months, respectively; HR 0.50; 95% CI 0.27, 0.95). Objective response rate was greater with tislelizumab (32.0%) versus chemotherapy (20.0%), and responses were more durable (median duration of response: 8.8 vs. 2.6 months, respectively). Fewer patients experienced ≥ grade 3 treatment-related adverse events with tislelizumab (24.0%) versus chemotherapy (47.8%). Tislelizumab demonstrated an improvement in health-related quality of life versus chemotherapy., Conclusions: As second-line therapy for advanced/metastatic ESCC, tislelizumab improved OS versus chemotherapy, with a favorable safety profile, in the Japanese patient subgroup, consistent with the overall population., Clinical Trial Registry: ClinicalTrials.gov: NCT03430843., (© 2024. The Author(s).)

Background and aims : Tea polyphenols, such as green tea polyphenols, have been extensively studied as agents that ameliorate cardiovascular disease and blood pressure in vitro and in animal studies. However, epidemiological evidence for the association of green tea consumption with hypertension (HTN) is inconsistent. In addition, such an association has not been prospectively examined in the general adult population, particularly among young women. Therefore, we designed a cohort study to examine whether green tea consumption increases the risk of HTN in premenopausal women. Methods and results : This prospective cohort study investigated 6633 premenopausal female participants without hypertension, cardiovascular disease, and cancer at the baseline. Green tea consumption was measured at the baseline using a validated food frequency questionnaire. Hypertension was confirmed with the SBP ≥140 mm Hg -1 or with the DBP ≥90 mm Hg -1 . Cox proportional hazards regression models were used to examine the association of green tea consumption with incident hypertension. A total of 488 first incident cases of hypertension occurred during 24 957 person-years of follow-up (median follow-up of 4.0 years). After adjustment for potential confounding variables, the multivariable hazard ratios (95% confidence intervals) for incident hypertension in premenopausal female participants with different green tea consumption frequencies were 1.00 (reference) for almost never, 0.84 (0.67, 1.07) for 1 cup per week, 1.02 (0.77, 1.35) for 2-6 cups per week, and 0.65 (0.44, 0.96) for ≥1 cup per day. Conclusions : The results from our prospective study indicate that the consumption of green tea is associated with a reduced risk of HTN in premenopausal women.

Background: The prospective studies on the effect of particular type of tea consumption, especially green tea, on depressive symptoms are limited., Objective: The aim of this study is to investigate the prospective association between green tea consumption and depressive symptoms in a large general adult population., Methods: This prospective cohort study investigated 7524 participants aged 25 to 90 years from May 2013 to December 2018 and they were free of cardiovascular disease, cancer, and depressive symptoms at baseline. Green tea consumption was obtained through a validated food frequency questionnaire. Depressive symptoms were assessed by using the Self-Rating Depressive Scale (SDS). The association between green tea consumption and depressive symptoms was analyzed by Cox proportional hazards regression models., Results: A total of 1064 first incident cases of depressive symptoms (SDS ≥45) occurred during 14,661 person-years of follow-up (median follow-up of 2.0 years). In the crude model, the hazard ratios (95% confidence intervals) were 1.00 (reference), 0.95 (0.81, 1.12), 0.97 (0.83, 1.14) and 0.95 (0.79, 1.14), respectively. After adjusting for demographic characteristics, lifestyle factors, and dietary intake, the multivariable adjusted hazard ratios (95% confidence intervals) were 1.00 (reference), 0.88 (0.74, 1.05), 0.84 (0.69, 1.02) and 0.78 (0.63, 0.97), respectively., Conclusions: The prospective study suggests that frequent green tea consumption is associated with a decreased risk of depressive symptoms in the general Chinese population., (Copyright © 2022. Published by Elsevier B.V.)

A novel picornavirus was isolated from the faeces of a diarrhoeic cow using MA-104 cells at the third blind passage. This virus, named Den1/2021/JPN, was completely sequenced using total RNA from the cell culture supernatant by deep sequencing. The genome of Den1/2021/JPN had a standard picornavirus genome organisation with conserved picornaviral motifs. The 5' untranslated region harboured a type-II internal ribosomal entry site. Den1/2021/JPN was most closely related to a bovine parechovirus (Bo_ParV) named cow/2018/4, which has been recently identified in publicly available databases. Phylogenetic analyses and pairwise sequence comparison revealed that Den1/2021/JPN and Bo_ParV cow/2018/4 clustered with parechoviruses and were most closely related to Parechovirus E identified in birds of prey, exhibiting nucleotide sequence similarity of 64.2-64.5 %, 58.6-59.7 % and 66.3-66.4 % in the polyprotein, P1 and 2C+3 CD coding regions, respectively. This study presents the first report on the isolation of Bo_ParV. Den1/2021/JPN and Bo_ParV cow/2018/4, which are candidates for a novel species in the genus Parechovirus .

To evaluate the morphological characteristics of flow void (FV) in the fellow eyes of the unilateral polypoidal choroidal vasculopathy (PCV). Fifty PCV fellow eyes (PCVF) and 31 age-matched normal ocular circulation controls were recruited in this retrospective study. The number of FV was analyzed according to the size in a centered 5 × 5 mm swept source optical coherence tomography angiography scans. We used indocyanine green angiography images to determine whether choroidal vascular hyperpermeability (CVH) has occurred. For the PCVF, the prevalence rate of CVH was 70% (35 of 50) The number of FVs was significantly lower in 400-25,000 μm 2 (P = 0.005), 400-500 μm 2 (P = 0.001), 525-625 μm 2 (P = 0.001) and 650-750 μm 2 (P = 0.018). compared to the controls. And showed no difference in size from 775 to 1125 μm 2 between the two groups. The area under the receiver operating characteristic curve of PCVF with CVH and controls was 0.94 (95% CI 0.88-1.00) (P < 0.001). We found that the number of small FVs was significantly lower in the PCV fellow eyes than that in the eyes with control group., (© 2021. The Author(s).)

The seven leading industrial countries, called the G7, are becoming a pivotal group to fulfil their emissions-reduction commitments to manage the climate crisis. This paper investigates the relationships between R&D intensity, globalization, and carbon emissions in the context of the G7 countries for the period from 1970 to 2017. Our analysis, which examines these relationships, focuses on the wavelet coherence approach to conduct time-frequency domain analyses. The empirical results show that there is heterogeneity across different time scales and frequencies for R&D intensity, globalization, and carbon emissions within each country. Specifically, R&D intensity and globalization are negatively correlated with carbon emissions for the G7 countries, except Japan, for which they are positive. The long-term correlations between R&D intensity, globalization, and carbon emissions are higher than those in the short- and medium-term periods. In addition, the multiscale connectedness network results reveal that the strongest bidirectional correlations exist between energy consumption, economic growth, and carbon emissions. Our results provide a useful reference for policymakers in the G7 countries to effectively regulate carbon emissions., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Introduction: Gastric cancer (GC) has a high incidence and mortality rate, especially in East Asians, and about 90% of GCs are adenocarcinomas. Histological and etiological heterogeneity and ethnic diversity make molecular subtyping of GC complicated, thus making it difficult to determine molecular division systems and standard treatment modalities. Limited cohorts from South Korea, Singapore, Australia, and Japan have been studied; however, the mutational landscape of gastric adenocarcinomas in Chinese patients is still unknown., Methods: We performed a targeted sequencing panel focusing on cancer-related genes and tumor-associated microorganisms of 529 gastric adenocarcinoma samples with matched blood controls. We identified 449 clinically relevant gene mutations., Results: Approximately 47.1% of Chinese patients with GC harbored at least one actionable mutation. The top somatic mutations were TP53, ARID1A, LRP1B, PIK3CA, ERBB2, CDH1, KRAS, FAT4, CCNE1, and KMT2D. Truncation mutations of ARID1A, KMT2D, RNF43, TGFBR2, and CIC occurred in patients with high tumor mutational burden. Gene amplifications of ERBB2, CCNE1, CDK12, and CCND1 were detected in patients with low tumor mutational burden. Pathway analysis revealed common gene alterations in the Wnt and PI3K/Akt signaling pathways. The ratio of patients with high microsatellite instability was significantly lower than other cohorts, and high microsatellite instability and Epstein-Barr virus (EBV)-positive features seemed mutually inclusive in Chinese patients with GC. In 44 (8.3%) patients, 45 germline mutations were identified, among which SPINK1 mutations, all SPINK1 c.194 + 2T > C, were present in 15.9% (7/44) of patients. Microorganisms found in Chinese patients with GC included Helicobacter pylori, EBV, hepatitis B virus, and human papillomavirus types 16 and 18., Conclusion: Identification of varied molecular features by targeted next-generation sequencing provides more insight into patient stratification and offers more possibilities for both targeted therapies and immunotherapies of Chinese patients with GC., Implications for Practice: This study investigated the genomic alteration profile of 529 Chinese patients with gastric adenocarcinoma by deep targeting sequencing, which might be the largest Chinese cohort on the genomic research of gastric adenocarcinoma up to now., (© 2021 The Authors. The Oncologist published by Wiley Periodicals LLC on behalf of AlphaMed Press.)

Background: The difference in growth and nutritional status, both important indices of population quality, between Chinese and Japanese children and adolescents is unknown., Aim: This study aimed to compare growth and nutritional status between Chinese and Japanese children and adolescents., Subjects and Methods: The height-for-age and BMI-for-age distribution of 9,226 children and adolescents aged 7-18 years from China and Japan were described with the Lambda Mu and Sigma method. Wasting, overweight and obesity were evaluated based on BMI-for-age cut-offs of the 2007 WHO Child Growth Reference., Results: For boys, the overall average height, weight and BMI of Chinese participants were 3.0 cm, 4.8 kg and 1.2 kg/m 2 greater compared with Japanese participants, respectively; for girls, these were 4.6 cm, 3.9 kg and 0.6 kg/m 2 , respectively. Compared with Japanese children, the 3rd, 50th and 97th percentiles of height-for-age, 1 Z -score, and 2 Z -score of BMI-for-age of Chinese children were greater, whereas the minus 2 Z -scores of Chinese children were less. The prevalence of wasting, overweight and obesity among Chinese participants was greater., Conclusions: Compared with Japanese children, Chinese children tended to be taller. The worrying burden of overweight, obesity and wasting was recognised among Chinese children.

In recent years, there is a trend of low C/N ratio in municipal domestic wastewater, which results in serious problems for nitrogen removal from wastewater. The addition of an external soluble carbon source has been the usual procedure to achieve denitrification. However, the disadvantage of this treatment process is the need of a closed, rather sophisticated and costly process control as well as the risk of overdosing. Solid-phase denitrification using biodegradable polymers as biofilm carrier and carbon source was considered as an attractive alternative for biological denitrification. The start-up time of the novel process using PCL (polycaprolactone) as biofilm carrier and carbon source was comparable with that of conventional process using ceramsite as biofilm carrier and acetate as carbon source. Further, the solid-phase denitrification process showed higher nitrogen removal efficiency under shorter hydraulic retention time (HRT) and low carbon to nitrogen (C/N) ratio since the biofilm was firmly attached to the clear pores on the surface of PCL carriers and in this process bacteria that could degrade PCL carriers to obtain electron donor for denitrification was found. In addition, solid-phase denitrification process had a stronger resistance of shock loading than that in conventional process. This study revealed, for the first time, that the physical properties of the biodegradable polymer played a vital role in denitrification, and the different microbial compositions of the two processes was the main reason for the different denitrification performances under low C/N ratio.

Background: This study aimed to compare the difference in cardiorespiratory fitness between Chinese and Japanese children and adolescents., Methods: Participants comprised 9025 children and adolescents aged 7-18 years from China and Japan. Cardiorespiratory fitness (CRF) was measured by performance in the 20 m shuttle run test (20mSRT) and estimated maximal oxygen consumption (VO 2max ). Differences in CRF between countries were evaluated by t -tests. Centile curves for the 20mSRT and VO 2max values were constructed for Chinese and Japanese children and adolescents, respectively, using the Lambda Mu and Sigma (LMS) method., Results: (1) For most of the age groups, the 20mSRT and VO 2max performances among Chinese participants were lower than among Japanese participants. (2) Japanese children had the most apparent gains in P 10 , P 50 , and P 90 VO 2max values in primary school; however, they gradually decreased in middle school. For Chinese girls, the P 10 , P 50 , and P 90 VO 2max values decreased gradually with age. (3) The VO 2max value among Japanese children increased; however, it decreased or remained flat among Chinese children in primary school., Conclusions: CRF among Chinese participants was lower than among Japanese participants while the VO 2max value showed different trends in primary school. Effective measures should be taken to improve CRF among children and adolescents.

Drosophila suzukii is an invasive fly of East Asian origin that has become a serious fruit pest worldwide. Classical biological control through the introduction of parasitoids from Asia could help reduce populations of D. suzukii in invaded regions. Little is known about the native parasitoids of the fly in Asia. Therefore, surveys for larval parasitoids of D. suzukii were carried out in China and Japan between 2015 and 2017. Parasitoids of D. suzukii and other fruit-inhabiting drosophilids (D. pulchrella and D. subpulchrella) that are probably attacked by the same parasitoid complex were found in four Chinese provinces and four Japanese prefectures. Larval parasitoids were obtained at most sites where D. suzukii was found, with parasitism varying from 0.0 to 75.6%. At least eight parasitoid species were reared. The most abundant and frequent parasitoids were the Figitidae Ganaspis cf. brasiliensis and Leptopilina japonica, but another Leptopilina species and at least five Braconidae species belonging to the genera Areotetes, Asobara and Tanycarpa were obtained in low numbers. Due to its likely restricted host range, the most promising parasitoid for biological control is Ganaspis cf. brasiliensis. However, its exact specificity and taxonomic status require future research.

A hybrid allele between the carboxyl ester lipase gene (CEL) and its pseudogene, CELP (called CEL-HYB), generated by nonallelic homologous recombination between CEL intron 10 and CELP intron 10', was found to increase susceptibility to chronic pancreatitis in a case-control study of patients of European ancestry. We attempted to replicate this finding in 3 independent cohorts from China, Japan, and India, but failed to detect the CEL-HYB allele in any of these populations. The CEL-HYB allele might therefore be an ethnic-specific risk factor for chronic pancreatitis. An alternative hybrid allele (CEL-HYB2) was identified in all 3 Asian populations (1.7% combined carrier frequency), but was not associated with chronic pancreatitis., (Copyright © 2016 AGA Institute. Published by Elsevier Inc. All rights reserved.)

Six spirostanol glycosides (1-6) and 12 known compounds (7-18) were isolated from the underground parts of Dracaena thalioides (Agavaceae). Their structures were determined by spectroscopic analysis, including 2D NMR spectroscopic data, and chemical transformations. The isolated compounds were evaluated for cytotoxic activity against HL-60 human leukemia cells. Compounds 1, 3-6, and 8-18 showed cytotoxicity against HL-60 cells, of which 10, a bisdesmosidic spirostanol derivative, showed potent cytotoxicity against HL-60 cells with an IC50 value of 0.38μM and induced apoptosis in HL-60 cells., (Copyright © 2014 Elsevier Ltd. All rights reserved.)

Background: In the past decade, scientific research has developed rapidly in China, but the growth seems to vary widely between different disciplines. In this study, we aimed to compare the quantity and quality of publications in urology and nephrology journals from USA, China and Japan., Methods: Journals listed in the "Urology and Nephrology" category of Science Citation Index Expanded subject categories were included. Scientific papers in these journals written by researchers from USA, Japan and China were retrieved from the "PubMed" and "Web of Knowledge" online databases., Results: The annual number of total scientific articles increased significantly from 2001 to 2010 in China, and has ranked second in the world since 2006. In the field of urology and nephrology, the annual number increased significantly from 2001 to 2010 in USA and China; but not in Japan. The share of articles increased significantly over time in China, decreased significantly in Japan, and remained unchanged in USA. In 2010, USA contributed 32.17% of the total world output in urology and nephrology field and ranked 1(st); Japan contributed 5.19% and ranked 5(th); China contributed 3.83% and ranked 9(th). Publications from USA had the highest accumulated IFs and the highest total citations of articles (USA>Japan>China, p<0.001). No significant difference was found in average IF among the three countries. USA published the most articles in the top 10 urology and nephrology journals (USA(35165)>Japan(6704)>China(2233), p<0.001). Researchers from USA published more clinical trials and randomized controlled trials than Japan and China (USA>Japan>China, p<0.001)., Conclusion: Although China has undergone significant increase in annual number and percentage of scientific publication in urology and nephrology journals in the past decade, it still lags far behind USA and Japan in the field of urology and nephrology in terms of quantity and quality.

Background: Identification of pathogen DNA from archaeological human remains is a powerful tool in demonstrating that the infectious disease existed in the past. However, it is very difficult to detect trace amounts of DNA remnants attached to the human skeleton, especially from those buried in a humid atmosphere with a relatively high environmental temperature such as in Asia., Methodology/principal Findings: Here we demonstrate Mycobacterium leprae DNA from archaeological skeletal remains in Japan by polymerase chain reaction, DNA sequencing and single nucleotide polymorphism (SNP) analysis. In addition, we have established a highly sensitive method of detecting DNA using a combination of whole genome amplification and polymerase chain reaction, or WGA-PCR, which provides superior sensitivity and specificity in detecting DNA from trace amounts of skeletal materials., Conclusion/significance: We have detected M. leprae DNA in archaeological skeletal remains for the first time in the Far East. Its SNP genotype corresponded to type 1; the first detected case worldwide of ancient M. leprae DNA. We also developed a highly sensitive method to detect ancient DNA by utilizing whole genome amplification.

A virus identified as a new pathotype of Papaya leaf distortion mosaic virus (PLDMV, P-TW-WF) was isolated from diseased papaya in an isolated test-field in central Taiwan, where transgenic papaya lines resistant to Papaya ringspot virus (PRSV) were evaluated. The infected plants displayed severe mosaic, distortion and shoe-stringing on leaves; stunting in apex; and water-soaking on petioles and stems. This virus, which did not react in enzyme-linked immunosorbent assay with the antiserum to the PRSV coat protein, infected only papaya, but not the other 18 plant species tested. Virions studied under electron microscope exhibited morphology and dimensions of potyvirus particles. Reverse transcription-polymerase chain reaction conducted using potyvirus-specific primers generated a 1,927-nucleotide product corresponding to the 3' region of a potyvirus, showing high sequence identity to the CP gene and 3' noncoding region of PLDMV. Search for similar isolates with the antiserum against CP of P-TW-WF revealed scattered occurrence of PLDMV in Taiwan. Phylogenetic analysis of PLDMV isolates of Taiwan and Japan indicated that the Taiwan isolates belong to a separate genetic cluster. Since all the Taiwan isolates infected only papaya, unlike the cucurbit-infecting Japanese P type isolates, the Taiwan isolates are considered a new pathotype of PLDMV. Susceptibility of all our PRSV-resistant transgenic papaya lines to PLDMV indicates that the virus is an emerging threat for the application of PRSV-resistant transgenic papaya in Taiwan and elsewhere.

Yeast Saccharomyces cerevisiae cells generally cannot synthesize biotin, a vitamin required for many carboxylation reactions. Although sake yeasts, which are used for Japanese sake brewing, are classified as S. cerevisiae, they do not require biotin for their growth. In this study, we identified a novel open reading frame (ORF) in the genome of one strain of sake yeast that we speculated to be involved in biotin synthesis. Homologs of this gene are widely distributed in the genomes of sake yeasts. However, they are not found in many laboratory strains and strains used for wine making and beer brewing. This ORF was named BIO6 because it has 52% identity with BIO3, a biotin biosynthesis gene of a laboratory strain. Further research showed that yeasts without the BIO6 gene are auxotrophic for biotin, whereas yeasts holding the BIO6 gene are prototrophic for biotin. The BIO6 gene was disrupted in strain A364A, which is a laboratory strain with one copy of the BIO6 gene. Although strain A364A is prototrophic for biotin, a BIO6 disrupted mutant was found to be auxotrophic for biotin. The BIO6 disruptant was able to grow in biotin-deficient medium supplemented with 7-keto-8-amino-pelargonic acid (KAPA), while the bio3 disruptant was not able to grow in this medium. These results suggest that Bio6p acts in an unknown step of biotin synthesis before KAPA synthesis. Furthermore, we demonstrated that expression of the BIO6 gene, like that of other biotin synthesis genes, was upregulated by depletion of biotin. We conclude that the BIO6 gene is a novel biotin biosynthesis gene of S. cerevisiae.

The presence of six gene 4 alleles (or VP4 genotypes) in human rotaviruses has been recognized. Using 16 representative cultivable human rotavirus strains, we confirmed the specificity of VP4 genotyping by polymerase chain reaction (PCR) using the nested oligonucleotides specific to each of the four representative gene 4 alleles. Using the PCR, we surveyed the gene 4 alleles of 199 human rotaviruses in stools collected in Japan and Thailand. Strains with the gene 4 allele, corresponding to P1A serotype, were shown to be the most prevalent, but two strains with P2 gene 4 allele and one strain with P3 gene 4 allele were detected in Thailand and in Japan, respectively.