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The Covid-19 pandemic has prompted countries to implement a wide range of specific cross-border security measures. The fear and anxiety induced by this crisis have rapidly expanded and contracted countries' understanding of 'self' and 'other'. This study examines Japan's shift from liberal pre-Covid-19 border policies to the most stringent border closure measures among the G7 countries during the pandemic. In this study, we argue that the pandemic-induced fear increased anxiety in Japan's public health safety identity, rapidly reshaping the conceptualization of the 'self' and 'other' in crisis scenarios. This shift aligns with Japan's historical narrative of combating unprecedented threats to public health. [ABSTRACT FROM AUTHOR]

Four-element triple-band systems have been studied and developed for long-term evolution (LTE) and 5G mid-band (4.5–4.9 GHz) operation. The presented multi-input multioutput (MIMO) consists of four planar which are excited with inset feeding. The system is designed on substrate (FR-4) with dielectric constant (εr) of 4.4 and dielectric loss (tan δ) 00.02. The complete measurements of implemented antenna are 150 × 150 × 1.6 mm3. This article provides a triple band to operate under LTE bands, such as 02.4 GHz, 03.7 GHz, and 04.5 GHz for |S11|≤ − 10 dB. This antenna is operating for LTE band 40 (2300–2400 MHz) at 2.4 GHz with impedance bandwidth of 58.6 MHz, LTEA band 46 (3700–4000 MHz) at 3.7 GHz with operating bandwidth of 87.1 MHz and 5G mid-band range for Japan (4500–4900 MHz) at 4.5 GHz with operating bandwidth 87.1 MHz. This MIMO provides > 21 dB isolation among the 4 elements operating under the operating band. Therefore, the antenna is appropriate for LTE/5G operations. [ABSTRACT FROM AUTHOR]

In this study, a web‐based questionnaire was sent to residents of the Kinki region in the westside of Japan (N = 1053), and a multinomial logit analysis was conducted according to their isolation and loneliness status to examine social prescriptions that would presumably contribute to the prevention of isolation and loneliness. By comparing the marginal effects of social prescriptions on those with four types of isolation and loneliness status through the discrete choice model, we discovered that social prescriptions that contribute to the prevention of isolation and loneliness differ according to individuals by isolation and loneliness status. For example, for those who are not isolated but lonely, an effective strategy would be to encourage involvement in social contribution activities in the community and form high‐quality human relationships expecting to alleviate loneliness. The results also showed that residents in isolated situations tend to be indifferent to social contribution activities and social prescriptions to encourage involvement in such activities have limitations. [ABSTRACT FROM AUTHOR]

T. dolabrata is a valuable conifer and an essential forestry species endemic to Japan. The essential oil extracted from the wood has a unique relaxing woody fragrance and possesses antifungal, and anti-allergic potentials. Given the potential of the plant wood to serve as a rich source of bioactive compounds and pharmacology, there is a need for a comprehensive understanding of the current state of knowledge on this medicinal plant. A systematic review adopting the PRISMA 2020 protocol with emphasis on studies carried out from January 2000 to December 2023 was conducted. The review focused on extraction techniques, phytochemistry, and therapeutic applications of wood biomass. Findings from the review have revealed the presence of various phytochemicals in the wood with terpenoids (Thujopsene and Hinikitiol) being the most abundant compounds. Among the methods reviewed, supercritical fluid extraction was reported as the method with the best extraction yield, and maceration was the cheapest and easiest method. The isolation methods used are mainly HPLC and column chromatography. The review also identified studies supporting the pharmacological potentials which include psychological and physiological activity, antifungal and antimicrobial activity, anti-allergic activity, cytotoxicity, and anti-tumor activity. However, there is a need for further research to unveil yet-to-be-identified compounds, optimize extraction methods using green techniques, and further validate therapeutic applications. T dolabrata wood emerges as a promising source of bioactive compounds, prompting exploration for sustainable extraction practices and expanded medicinal applications. • The extraction methods applied to T. dolabrata wood include both conventional and modern techniques. • The major compounds currently identified in the woods of T. dolabrata are mainly terpenoids and the most abundant compounds are thujopsene and hinokitol. • The current research on the pharmacological potentials of T. dolabrata wood includes allergy, antimicrobial, psychological, physiological, cytotoxicity, and anti-tumor activity. • Thujopsene and Hinokitiol are responsible for the major pharmacological potential of the wood. [ABSTRACT FROM AUTHOR]

Porcine adenoviruses (PAdVs) are distributed in pig populations and classified into five immunologically distinct serotypes (PAdV-1 to 5). In this study, a PAdV was isolated from a fecal sample of wild boar for the first time. Whole-genome analysis revealed that this strain (Ino5) has sequence homology (approximately > 93%) throughout the genome with the PAdV-5 strain HNF-70 that was isolated from a pig in Japan in 1987, except for the hexon, E3 612R, and fiber coding regions. Two possible recombination breakpoints were detected in the hexon and E3 612R regions, which were found to have reduced GC content. Structural prediction analysis showed that a part of the hexon protein corresponding to the tower region of Ino5 had structural differences when compared with HNF-70, suggesting antigenic heterogeneity between these strains. PAdVs were detected in 1.77% (2/113) and 12% (12/100) of the fecal samples from wild boars and pigs collected in Japan by PCR, respectively. Phylogenetic analyses of the hexon and fiber genes revealed that some samples showed different grouping in the hexon and fiber genes, suggesting that these viruses have recombination events. These findings suggest that the PAdV-5 has evolved with homologous recombination events in the same manner as human adenoviruses among not only pig populations, but also wild boars in Japan. [ABSTRACT FROM AUTHOR]

In order to improve the reliability of the traditional doubly salient electro‐magnetic generators (DSEG) and realize the isolation of interphase magnetic circuit, a U‐shaped rotor stator excitation generator (UR‐SEG) for vehicle applications is proposed in this paper. The machine has the interphase physical isolation and short flux path structure because of the U‐shaped rotors. According to the theoretical analysis of the fault‐tolerant characteristics of this UR‐SEG, the characteristic of the phase electromotive force (EMF) of the UR‐SEG completely overlaps with the EMF of front and rear phase in the entire power generation stage is revealed. Then the flux linkage, EMF, output voltage of both normal and faulty states are comparative investigated using the two‐dimensional finite element method (FEM). The output characteristics of the normal state and four faulty states of the machine with different exciting currents are researched, and the influence rules of fault on output characteristics are obtained. Finally, the outstanding fault‐tolerant characteristics of the UR‐SEG were verified by the experimental results. © 2022 Institute of Electrical Engineers of Japan. Published by Wiley Periodicals LLC. [ABSTRACT FROM AUTHOR]

Nationwide depopulation in modern Japan has led to contrasting changes over the last half‐century: shrinking human communities and recovering populations of Japanese macaques (Macaca fuscata). These changes have triggered macaque damage to public livelihoods, often resulting in decision‐making directed toward strictly regulating macaque populations. To optimize conservation interventions required today, we aimed to identify macaque habitats with conservation priorities to enhance population connectivity. To quantify the current population connectivity, we conducted a least‐cost path analysis by assigning an inverse of habitat suitability as resistance. For this, we collected geo‐information on home ranges of 2288 troops, accounting for >70% of the total number of troops in Japan. Moreover, we composed a minimum spanning tree using least‐cost paths to provide optimal population connectivity. Consequently, the modeled resistance showed that, although human‐induced barriers (i.e., flat areas where people frequently reside) and geographical barriers (i.e., alpine regions >1500 m above sea level) had the highest dispersal cost, macaques presented a high plasticity in the occupation of different forest types. The minimum spanning tree, composed of 77 modeled corridors, detected the vulnerability of population connectivity in western Japan and assigned conservation priorities to these isolated populations to preserve the stepping‐stone paths of macaque movement. [ABSTRACT FROM AUTHOR]

Late capitalist imaginaries of Japan include characterisations of an insecure political economy with significant alterations to traditional norms, interpersonal relationships, and identities. Since Japan’s neoliberal reform in the 1990s an insidious narrative of the hikikomori, characterised by perceived personal failure, social reclusiveness, and mental illness, has become commonplace in the mediascape. This paper offers a discussion on othering as emerging in prevailing cultural and counter-cultural narratives of hikikomori, and in an attempt to challenge traditional orthodoxies around the individual and society, explores the opportunities provided by possible alternatives. In trying to unlock the complexity of self-reclusion, this paper argues that it is impossible to understand this phenomenon in normative terms and highlights the ways in which these manifestations of self as other are being contested and challenged in cultural media texts. In problematising the pre-eminence of a distinct set of narratives that interact to discursively frame hikikomori, our intention is not to add to the existing explanatory claims, but rather to offer alternate approaches for understanding the social location of hikikomori within the public imaginary. Hikikomori exists in a liminal space of public understanding and private experience of self; simultaneously socially integrated yet set apart from society. Its existence, or more specifically the social reactions to its existence, highlights a need for a reconfiguration of traditional notions of the individual and society. [ABSTRACT FROM AUTHOR]

Since 2003, various viruses from the subfamily Megavirinae in the family Mimiviridae have been isolated worldwide, including icosahedral mimiviruses and tailed tupanviruses. To date, the evolutionary relationship between tailed and nontailed mimiviruses has not been elucidated. Here, we present the genomic and morphological features of a newly isolated giant virus, Cotonvirus japonicus (cotonvirus), belonging to the family Mimiviridae. It contains a linear double-stranded DNA molecule of 1.47Mb, the largest among the reported viruses in the subfamily Megavirinae, excluding tupanviruses. Among its 1,306 predicted open reading frames, 1,149 (88.0%) were homologous to those of the family Mimiviridae. Several nucleocytoplasmic large DNA virus (NCLDV) core genes, aminoacyl-tRNA synthetase genes, and the host specificity of cotonvirus were highly similar to those of Mimiviridae lineages A, B, and C; however, lineage A was slightly closer to cotonvirus than the others were. Moreover, based on its genome size, the presence of two copies of 18S rRNA-like sequences, and the period of its infection cycle, cotonvirus is the most similar to the tupanviruses among the icosahedral mimiviruses. Interestingly, the cotonvirus utilizes Golgi apparatus-like vesicles for virion factory (VF) formation. Overall, we showed that cotonvirus is a novel lineage of the subfamily Megavirinae. Our findings support the diversity of icosahedral mimiviruses and provide mechanistic insights into the replication, VF formation, and evolution of the subfamily Megavirinae. IMPORTANCE We have isolated a new virus of an independent lineage belonging to the family Mimiviridae, subfamily Megavirinae, from the fresh water of a canal in Japan, named Cotonvirus. In a proteomic tree, this new nucleocytoplasmic large DNA virus (NCLDV) is phylogenetically placed at the root of three lineages of the subfamily Megavirinae--lineages A (mimivirus), B (moumouvirus), and C (megavirus). Multiple genomic and phenotypic features of cotonvirus are more similar to those of tupanviruses than to those of the A, B, or C lineages, and other genomic features, while the host specificity of cotonvirus is more similar to those of the latter than of the former. These results suggest that cotonvirus is a unique virus that has chimeric features of existing viruses of Megavirinae and uses Golgi apparatus-like vesicles of the host cells for virion factory (VF) formation. Thus, cotonvirus can provide novel insights into the evolution of mimiviruses and the underlying mechanisms of VF formation. [ABSTRACT FROM AUTHOR]

Objective: This study intends to clarify the effect of regional disaster preparedness education on efforts to prevent the isolation of older people in the event of a disaster. Design and Sample: This quasi‐experimental study involved participants aged 65 and above. The intervention group (n = 35) and the comparison group (n = 61) were compared in the first survey and another one month later. Measurements: The survey items assessed aspects such as awareness of and actions related to isolation preparedness, and awareness of support needed by others in the event of a disaster. The results were compared between the two groups. Intervention: The educational program was based on the transtheoretical model of health behavior change and focused on preventing isolation in the event of a disaster, by connecting vulnerable people with their neighbors. Puppet shows and group work were used as part of the program. Results: A month after the program implementation, the intervention group had significantly higher awareness of and actions related to isolation preparedness than the comparison group. Conclusions: Disaster preparedness education invokes a responsibility toward the prevention of isolation in the disaster and perceives it as a community issue and encourages individuals to support others. [ABSTRACT FROM AUTHOR]

Aim: This study aimed to identify patterns in mutual aid relationships between older people living alone and their relatives and neighbors, and to examine the impact of mutual aid relationship patterns on mental health and perceived isolation through the mediating effect of subjective economic status. Methods: We conducted a survey with 5000 randomly selected adults aged 65 years or older, from the five official senior care service areas of city A in Saitama Prefecture, Japan. Valid responses were received from 3941 participants (78.8%), of whom 436 participants from single‐person households constituted the study sample. Results: Latent class analysis revealed four types of mutual aid: family; family and intra‐generational neighbors; no mutual aid; and family and multi‐generational neighbors. Regression analysis showed that the interaction effect between family and neighbors' mutual aid and subjective economic status was a significant predictor of depressive symptoms. Simple slope analysis clarified that subjective economic status had a negative effect on depressive symptoms for the non‐family/neighbors group, but not for the family/neighbors group. Furthermore, the interaction term between no mutual aid and subjective economic status was negatively associated with the fear of future isolation. Finally, the significantly negative effect observed was greater for the no mutual aid group than for the non‐no mutual aid group. Conclusions: Mutual aid relationships are effective in improving the mental health and decreasing the fear of future isolation of older adults living alone who experience low economic status. Geriatr Gerontol Int 2021; 21: 555–560. [ABSTRACT FROM AUTHOR]

Isolation is one of the most critical factors related to older people's health. However, to prevent COVID-19, community socialisation among the elderly has halted. Because of older people's limited access to information technology, they cannot maintain external communication easily, and even several weeks of isolation from others could be detrimental to their health. How we sustain communication with and among the elderly is currently and will continue to be a considerable challenge in community work in Japan. In addition to contextualised information on Japanese community care for social welfare before and after the pandemic, the case of a social worker and her community is introduced. From her practices, wisdom in communication processes that can make communities more resilient to crisis can be gained and be of use in Japan and other Asian countries. [ABSTRACT FROM AUTHOR]

Shiga toxin-producing E. coli (STEC) is an important foodborne pathogen worldwide. It is necessary to control and prevent STEC contamination on beef carcasses in slaughterhouses because STEC infection is associated with beef consumption. However, the frequencies of STEC contamination of beef carcasses in various slaughterhouses in Japan are not well known. Herein, we investigated the contamination of beef carcasses with STEC in slaughterhouses to assess the potential risks of STEC. In total, 524 gauze samples were collected from the surfaces of beef carcasses at 12 domestic slaughterhouses from November 2020 to February 2023. The samples were measured for aerobic plate counts and tested for pathogenic genes (stx and eae) and major O-serogroups (O26, O45, O103, O111, O121, O145, and O157) by real-time PCR screening. Subsequently, immunomagnetic separation (IMS) was performed on samples positive for stx, eae, and at least one of the seven O-serogroups of STEC. Isolation process without IMS was performed on samples positive for stx, including those subjected to IMS. STEC O157:H7 and stx-positive E. coli other than serotype O157:H7 were isolated from 0.6% and 4.6% of beef carcass surfaces, respectively. Although the STEC O157:H7 isolation rate was low and stx-positive E. coli other than serotype O157:H7 belonged to minor O-serogroups, the results mean a risk of foodborne illness. Furthermore, a moderate correlation was observed between aerobic plate counts and detection rates of stx-positive samples by real-time PCR screening. The STEC O157:H7 isolated facilities showed higher values on aerobic plate counts and detection rates of stx-positive samples than the mean values of total samples. Therefore, these results suggest that it is important to evaluate hygiene treatments against beef carcasses for the reduction of STEC contamination risk, particularly in facilities with high aerobic plate counts., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

This research focused on in vitro and in vivo biocontrol of two major rice diseases, Bacterial Leaf Blight caused by Xanthomonus oryzae pv oryzae (Xoo) and Sheath Blight caused by Rhizoctonia solani. Furthermore, the soil of paddy fields in Kansai region, Japan was analysed for the presence of these pathogens, and their microbial communities were evaluated according to the location and management systems. For biocontrol, the rhizospheric bacteria were isolated and molecularly identified. The results indicated that DAR17225040 and DAR17225017 were 99% similar to Bacillus aryabhattai and Bacillus megaterium. The isolated bacteria were able to suppress Bacterial Leaf Blight and Sheath Blight disease 78% and 86% respectively, under greenhouse conditions. The 16S amplicon sequencing (Illumina MiSeq 250bp) was used to analyse the bacterial community in paddy fields of soil taken from five locations under conventional and organic systems. Xoo and R. solani were detected in sampled soil, however, no clear difference was noted, regarding systems. From amplicon sequencing data, Chloroflexi, Acidobacteria, Proteobacteria, Actinobacteria, Planctomycetes were the dominant phyla of rice soil in Kansai region. The phylum Kazan-3B-28 was found more in organic system than conventional. The bacterial community was more affected by location and soil type than by management systems. [ABSTRACT FROM AUTHOR]

Over the past 40 years, Japan has spread a definite condition: hikikomori. The term derives from hiku, "pull back", and komoru, "island", and describes a form of voluntary social withdrawal that involves adolescents. These subjects avoid social commitment, school education and friendships with an associated digital dependence. The causes identified depend on a cultural/educational and family system in which individual identity is subordinated to social identity, causing isolation. Early identification of hikikomori and above all its differentiation from other syndromes appears necessary to avoid inadequate diagnosis and interventions. This study stems from the intention to outline the phenomenon starting with the presentation of the characteristics of the phenomenon, focusing on possible causes and risk factors, then explain the psychological therapy based on the systemic-relational approach. A clinical case will be presented according to principles of the systemic-relational intervention. The subject, with a psychopathological diagnosis that can be linked to hikikomori and digital dependence, showed a dysfunctional family structure that has been treated by family psychotherapy. At the follow-up visit the patient showed new interpersonal skills by improving management and problem-solving skills. [ABSTRACT FROM AUTHOR]

The article discusses the issue of middle-aged Hikikomori individuals who have withdrawn from society for extended periods. It emphasizes the need for support methods that address the unique circumstances of middle-aged Hikikomori people and presents a case study of a 43-year-old man who reconnected with society after 26 years of withdrawal. The turning point for this individual was realizing his own and his parents' aging, which motivated him to seek support.

Escherichia albertii has increasingly been recognized as an important emerging zoonotic enteropathogen. Raccoon is shown to be one of the most vital reservoirs of this pathogen. E. albertii has been detected in 993 (62%) out of 1,606 wild raccoons in Osaka, Japan from 2017 to 2020 by Eacdt-PCR. The detection rate of E. albertii was increased from May to December (winter) and gradually decreased from January to April (spring). Furthermore, we could isolate E. albertii from 30% (196/664) of Eacdt-PCR positive samples and the monthly isolation rate seems to correlate with its detection rate. These data indicate that there is a seasonality regarding the prevalence of E. albertii in wild raccoon being higher in winter and lower in spring.

Seasonal changes in the diversity of tea plant endophytic fungi and the effects of sample storage conditions on detection were analyzed. Tea leaves were collected from the Saitama Tea Research Institute in Japan during winter (January 2020) and summer (August 2020). The effects of storage temperature (5, 10, 20, 25, and 30°C) and durations (1, 2, 3, 4, 5, 6, and 7 days) on endophytic fungal diversity and community structure were investigated. In summer, storage period and temperature did not affect the fungal colonization rate, frequency, and composition. In winter, storage temperature and period significantly affected the endophytic community structure. Fungal diversity was higher in winter than in summer. Positive relationships between diversity index and storage temperature and period were observed in winter, whereas the opposite trend was observed in summer. Our findings provide insight into the ecology of foliar endophytes of tea plants and the importance of proper sample collection and storage for microbiome studies., (© The Author(s) 2024. Published by Oxford University Press on behalf of FEMS.)

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Habitat fragmentation caused by damming can greatly reduce the population viability of aquatic organisms, with smaller fragmented populations at higher risk of extinction due to increased demographic, genetic, and environmental stochasticity. However, empirical evidence demonstrating that smaller natural populations are more vulnerable to extinction is limited. We studied the vulnerability to extinction of white-spotted charr (Salvelinus leucomaenis) populations in 30 dammed-off streams in Oshima Peninsula, southwestern Hokkaido Island, Japan, by comparing the incidence of charr populations in streams between 1999 and 2014. Using electrofishing and environmental DNA surveys, we identified three localized extinctions, with the probability of extinction increasing with decreasing watershed area (our surrogate for habitat size). We also found a new population in one dammed-off stream in which white-spotted charr were previously unknown, after installation of a fish ladder, indicating the capacity of white-spotted charr to recolonize reconnected habitat in a short period. Our results suggest that localized extinction of white-spotted charr in small dammed-off streams is ongoing, but that appropriate fish migration corridors can reduce localized extinction risk and increase the probability of species persistence. [ABSTRACT FROM AUTHOR]

Ticks are blood-sucking ectoparasites that transmit zoonotic pathogens to humans and animals. Ticks harbor not only pathogenic microorganisms but also endosymbionts. Although some tick endosymbionts are known to be essential for the survival of ticks, their roles in ticks remain poorly understood. The main aim of this study was to isolate and characterize tick-borne microorganisms from field-collected ticks using two arthropod cell lines derived from Ixodes scapularis embryos (ISE6) and Aedes albopictus larvae (C6/36). A total of 170 tick homogenates originating from 15 different tick species collected in Japan were inoculated into each cell line. Bacterial growth was confirmed by PCR amplification of 16S ribosomal DNA (rDNA) of eubacteria. During the 8-week observation period, bacterial isolation was confirmed in 14 and 4 samples using ISE6 and C6/36 cells, respectively. The sequencing analysis of the 16S rDNA PCR products indicated that they were previously known tick-borne pathogens/endosymbionts in three different genera: Rickettsia, Rickettsiella, and Spiroplasma. These included four previously validated rickettsial species namely Rickettsia asiatica (n = 2), Rickettsia helvetica (n = 3), Rickettsia monacensis (n = 2), and Rickettsia tamurae (n = 3) and one uncharacterized genotype Rickettsia sp. LON (n = 2). Four isolates of Spiroplasma had the highest similarity with previously reported Spiroplasma isolates: Spiroplasma ixodetis obtained from ticks in North America and Spiroplasma sp. Bratislava 1 obtained from Ixodes ricinus in Europe, while two isolates of Rickettsiella showed 100% identity with Rickettsiella sp. detected from Ixodes uriae at Grimsey Island in Iceland. To the best of our knowledge, this is the first report on successful isolation of Rickettsiella from ticks. The isolates obtained in this study can be further analyzed to evaluate their pathogenic potential in animals and their roles as symbionts in ticks. [ABSTRACT FROM AUTHOR]

The Entoloma clypeatum species complex, known as " Harushimeji " in Japan, associates with Rosaceae and Ulmaceae plant species. In this study, we successfully isolated cultures of this fungal group via basidiospore isolation from tentative four Harushimeji species using modified Norkrans's C (MNC) medium and MNC medium containing n-butyric acid. Colony formations were observed on 22 of 25 basidioma samples; however, most exhibited slow and unsteady growth. The isolated mycelia contained dikaryotic hyphae and were identified through molecular phylogenetic analyses of the internal transcribed spacer region of fungal ribosomal RNA. Six isolates showing steady growth were deposited in the fungal culture collection of the Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University, Japan. This result indicates that basidiospore isolation is a useful method for obtaining Harushimeji strains. • Cultures of tentative four Entoloma species were isolated from basidiospores. • Supplying n-butyric acid into medium facilitated isolation of E. cf. clypeatum. • All of established Entoloma isolates were consisted by dikaryotic hyphae. • Established strains were analyzed phylogenetically. [ABSTRACT FROM AUTHOR]

The family Marseilleviridae , defined as a group of icosahedral double-stranded DNA viruses with particle size of approximately 250 nm and genome size of 350–380 kbp, belongs to the nucleo-cytoplasmic family of large DNA viruses. The family Marseilleviridae is currently classified into lineages A–E. In this study, we isolated 12 or 15 new members of the family Marseilleviridae from three sampling locations in Japan. Molecular phylogenetic analysis of the MCP genes showed that the new viruses could be further classified into three groups, hokutoviruses, kashiwazakiviruses, and kyotoviruses. Hokutoviruses were closely related to lineage B, kyotoviruses were related to lineage A, and kashiwazakiviruses were also classified into lineage B but a new putative subgroup of lineage B, revealing the diversity of this lineage. Interestingly, more than two viruses with slightly different MCP genes were isolated from a single water sample from a single location, i.e., two hokutoviruses and one kashiwazakivirus were isolated from a small reservoir, five kashiwazakiviruses from the mouth of a river, and five kyotoviruses from fresh water of a river, suggesting that several milliliters of water samples contain several types of giant viruses. Amoeba cells infected with hokutoviruses or kashiwazakiviruses exhibited a "bunch" formation consisting of normal and infected cells similarly to a tupanvirus, whereas cells infected with kyotoviruses or tokyovirus did not. These results suggest the previously unrecognized local diversity of the family Marseilleviridae in aquatic environments. [ABSTRACT FROM AUTHOR]

Abstract Toxoplasma gondii is a protozoan parasite that causes fatal disease in New World monkeys. Several reports have described outbreaks of toxoplasmosis in squirrel monkeys. Here, we report the death of four squirrel monkeys in a captive colony from acute toxoplasmosis, one of which developed toxoplasmosis about 1 year after the initial outbreak. Serum anti– T. gondii antibody was detected by a latex agglutination test in the animals, and one presented seropositive before clinical signs were observed. Macroscopically, the lungs were severely affected and three animals showed pulmonary edema. Microscopically, interstitial pneumonia was observed in all animals. In the liver and heart, multifocal mononuclear cell infiltration with necrosis was detected. Parasite loading tended to be higher in the lungs, liver and heart than in the spleen, kidney and brain. The parasite was isolated from the brain of one animal and this isolate showed type II restriction patterns in the SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2 and PK1 genes of T. gondii and type I restriction patterns in the L358 and Apico genes by PCR-Restriction Fragment Length Polymorphism analysis. The clinical signs were reduced in mice infected with this isolate compared with those infected with reference type II strain PLK in a bioassay. To our knowledge, this is the first report of isolation of the parasite from squirrel monkeys in Japan and offers the opportunity for genomic and pathogenic analyses to aid our understanding of acute toxoplasmosis. Graphical abstract Unlabelled Image Highlights • Death of some squirrel monkeys in a captive colony were from acute toxoplasmosis. • Three animals showed pulmonary edema. • Isolate showed type II restriction patterns in the GRA6 , SAG3 and BTUB genes. • Virulence of the isolate was lower than reference type II strain in mouse. [ABSTRACT FROM AUTHOR]

Objectives In Japan, approximately 7000 smear-positive tuberculosis (TB) cases were reported in 2014. According to the law, such patients must be isolated in a TB ward until they become at least sputum smear-negative. However, there are some recalcitrant patients who are difficult to isolate because of problematic behaviors. This study was conducted to characterize recalcitrant TB patients and determine why they left the hospitals and how they were followed up after they left. Study design Cross-sectional study. Methods We sent a self-administered questionnaire to the hospitals in Japan with over 11 TB isolation beds, asking about recalcitrant TB inpatients from April 2013 through March 2014, in terms of demographic information, the reasons why they left, and how they were followed up. Results A total of 38 recalcitrant patients were reported, of whom 15 (39.5%) self-discharged, eight (21.0%) were discharged, and 15 (39.5%) were transferred. Thirty-three (86.8%) were males. The main reason (52.6%) why the patients were considered recalcitrant was related to psychiatric problems. However, nine (23.6%) patients physically assaulted or insulted the staff members. Thirty-six (94.7%) were able to continue anti-TB treatment after they left the original hospital. Conclusion Although the number of recalcitrant TB patients who were abusive was small, there should be TB facilities with law enforcement officials readily available to enforce isolation. [ABSTRACT FROM AUTHOR]

In the spring of 2015, two stallions reared in Farms A and B in Hokkaido in Japan showed symptoms of equine coital exanthema. Equine herpesvirus 3 (EHV-3) was isolated from penis swab samples of both stallions, and the isolates from each stallion in Farms A and B were designated as SS-1 and YS-1 strains, respectively. BamHI restriction profiles of SS-1 and Japanese reference strain Iwate-1 were indistinguishable, but the BamHI-A fragment of YS-1 was larger than those of SS-1 and Iwate-1 by 1.9 kbp because of the lack of two BamHI sites. Nucleotide sequence analyses of glycoprotein G (gG), gB, gC and VP13/14 coding regions revealed that SS-1 and YS-1 had 99.77% to 100% identities to each other. These results suggested that the origins of SS-1 and YS-1 were different. For a sero-epidemiological survey, serum neutralizing tests using SS-1 against 319 sera of horses from eight farms in Hokkaido were conducted. Six of the eight farms were EHV-3 antibody-positive, and positive rates ranged from 2.6% to 17.6%. To determine the infection time of four EHV-3 antibody-positive horses, a retrospective study was conducted. Infection time of the four horses was in the breeding season, and re-infection or reactivation of latently infected EHV-3 might have occurred in one horse. However, these four horses had never shown any clinical symptoms. The results suggested that several EHV-3 strains are distributed in Japan and that infection is maintained widely in horses without clinical symptoms. [ABSTRACT FROM AUTHOR]

Background: Patient and staff cohorting is part of a bundle approach in the response to multi-drug-resistant organisms, but its effectiveness is not fully clarified. This study compared the risks of acquiring vancomycin-resistant Enterococcus faecium (VREfm) at a hospital during a VREfm outbreak based on contact characteristics in order to better understand the effectiveness of cohorting., Methods: Exposure came from contact with patients with VREfm (infectors), including existing patients with VREfm and patients who acquired VREfm during the study period. Contact was defined as length of contact time, degree of sharing space, and care by the same nurses as those caring for infectors between January and March 2018. The outcome was VREfm acquisition as determined through monthly stool or rectal screening cultures. Incidence rates were calculated based on contact patterns, and incidence rate ratios (IRRs) were compared., Findings: Among 272 inpatients (4038 patient-days), 43 patients acquired VREfm with the same or similar pulsotype. Incidence rates were 8.45 per 1000 patient-days when susceptible inpatients were on the same ward as an infector but cared for by different nurses (reference), 16.96 when susceptible inpatients were on the same ward as an infector and cared for by the same nurses [IRR 2.01, 95% confidence interval (CI) 0.62-10.28], and 52.91 when susceptible inpatients shared a room with an infector (IRR 6.26, 95% CI 1.61-35.40)., Conclusion: Compared with susceptible inpatients in a different room from infectors and not being cared for by the same nurses, the risk of VREfm acquisition could be six times higher for susceptible inpatients who are in the same room as infectors, and could be double for susceptible inpatients cared for by the same nurses as infectors., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

This paper explores the experience of angst (ikizurasa) among youth in longterm unemployment in post-industrial Japan, and proposes a model for supporting them. Currently, the dominant model for unemployment support consists of activation policies, which assume that users can identify their problems and clarify their needs in job seeking. However, for youth in situations of long-term unemployment, the effectiveness of these policies is limited. This paper argues that indirect support which focuses on (re)constructing human relationships through sharing narratives is a more effective way to help such youth mitigate their angst. In-depth interviews with two participants in a Self-Help Group for youth affected by long-term unemployment, as well as participant observations of the group, reveal how a narrative approach allowed participants to build new senses of self founded in realities shared by others. The analysis shows how these developments were crucial to helping the participants to find employment that suited their situations and needs. [ABSTRACT FROM AUTHOR]

Corona virus disease has engulfed the globe for well over one year now. Multiple strategies to control the pandemic have been in practice in different countries, ranging from strict curfew like lock down to selective lock down (also called smart lock down) to no lock down at all. Proponents of no lock down favored the hypothesis of 'herd immunity'. In Japan and some other developed countries, the main emphasis has been on identification of positive case through mass testing. Although, if it made any difference in the morbidity or mortality, is anyone's guess. However, the use of mask by all and identification and separation of the infective (not all infected) persons has led Japan to control the spread better than many other countries. The author emphasizes that we shall need to continue our all-out efforts to defeat the virus for many more months if not years. [ABSTRACT FROM AUTHOR]

In Japan, there is no national 24-hour home care system for people with severe impairments. Despite this fact, a small number of people with Duchenne muscular dystrophy on home mechanical ventilation pursue independent living. Therefore, our aim was to better understand the process by which these individuals arrived at this goal for independence (i.e., choosing to live at home in Japan instead of in special sanatoriums that provide sufficient support and care). Twentyone participants were interviewed in 2011 and 2013. The interviews were recorded, transcribed, and analysed following a grounded theory approach. These individuals placed particular emphasis on their personal choice regarding where and how they live as well as on whom they depend. Therefore, the core element underlying participants' goals for independent living was self-reliant independency. To improve their social inclusion, the strategies used by the participants to retain their autonomy in an underdeveloped Japanese welfare system by establishing relationships with people in their communities can prevent them from experiencing social isolation. This could serve as an example to their counterparts in other countries. [ABSTRACT FROM AUTHOR]

Quarantine imposed due to COVID-19 infection can exacerbate psychological distress, and it is important for a public mental health agency to identify factors that are predictive of high psychological distress in such situation. The aim of this study was to investigate whether gender, age, and the presence or absence of infectious disease symptoms affected psychological distress among asymptomatic or mildly ill COVID-19 patients who were quarantined. Participants were 436 asymptomatic or mildly symptomatic COVID-19-infected patients who were quarantined in a treatment facility between 1 May 2020 and 30 September 2021. We used Quantification Theory I analysis to investigate the effects of gender, age, and the nature of infectious disease symptoms on psychological distress. The results of the analysis showed that the contribution rate was 0.06. Among gender, age, presence of symptoms, and the nature of symptoms, age had the greatest effect on psychological distress, and being in one's teens to thirties was considered to exacerbate psychological distress the most. According to the results, the psychological distress of asymptomatic and mildly symptomatic COVID-19 patients isolated was affected by gender, age, and symptomology, especially due to age differences. However, the impact of these items on psychological distress was not considered significant.

Abstract: Five cytotoxic macrolides, aplyronines D–H (4–8), were isolated from the Japanese sea hare Aplysia kurodai. They are new congeners of the antitumor compound aplyronine A (1), which was previously isolated from the same organism. Their structures were determined by spectroscopic analysis (NMR and MS). The cytotoxicity of these new compounds was evaluated in comparison with that of aplyronines A–C (1–3), suggesting the importance of the 7-O-seryl ester group for mediating the potent cytotoxicity of aplyronines. [Copyright &y& Elsevier]

The article discusses the results of a study that examined the Koala retrovirus (KoRV) infection in Queensland koalas housed at the Kobe Municipal Oji Zoo in Japan. A discussion of materials and methods is given, including culture of human embryonic kidney (HEK) 293T cells, genomic DNA sampling from whole blood and koala lymphocytes culture. The study observed positive, highly homologous bands of env and pol genes in all koalas. It concludes that koalas in which KoRV was isolated by inoculation showed clinical symptoms.

Abstract: We describe the characterization of polyhydroxyalkanoate (PHA)-producing bacteria isolated from an ammunition-polluted soil in Kitakyushu City, Japan. Over 270 strains were evaluated for PHA accumulation based on a colony staining method using Nile red. Of these, nine strains were selected based on the intensity of Nile red fluorescence and the cells were quantitatively analyzed for PHA by gas chromatography. PHA accumulation was observed in five strains, all of which are inferred to be close to the Bacillus cereus group according to 16S rDNA sequence analysis. Interestingly, these strains produced a PHA copolymer, poly(3-hydroxybutyrae-co-3-hydroxyvalerate) [P(3HB-co-3HV)], with a 3HV fraction up to 2 mol% with glucose as a carbon source. Further characterization was performed on one isolate, B. cereus YB-4. Gel permeation chromatography analysis revealed that the number of average molecular weights of PHA accumulated in B. cereus YB-4 drastically changed from 722,000 to 85,000 over a 72-h cultivation period. Furthermore, the PHA synthase genes were cloned and the deduced amino acid sequences were determined. This study provides new insights into PHA biosynthesis by members of the B. cereus group. [Copyright &y& Elsevier]

Abstract: Azaspiracid-2 was isolated from a marine sponge Echinoclathria sp. collected off Amami-Oshima as the predominant cytotoxic constituent. A combination of HPLC using ODS, GS320, and Phenylhexyl stationary phases permitted the purification without using acid or inorganic additives in the mobile phase. Azaspiracid-2 exhibited potent cytotoxicity against P388 cells with an IC50 value of 0.72ng/mL and caused S phase arrest on the cell cycle. [Copyright &y& Elsevier]

The article discusses economic aspects relating to the local mollusk industry in the tropical areas of China. The article focuses specifically on the noble scallop, Chlamys nobilis, found in southern China and Japan. Aquaculture interventions to increase the population of scallops include studying the genetic diversity and stock analysis of this species, as well as application of microsatellite markers. Also discussed are the relatively medium to low levels of polymorphism in terms of number of alleles in reference to the noble scallop.

• Rabbit hepatitis E virus (HEV) circulates among feral rabbits in Japan. • Persistent HEV infection was observed in a rabbit experimentally infected with the HEV isolate JP-59. • Further studies are required to determine the zoonotic risk of rabbit HEV. Rabbit hepatitis E virus (HEV) has been detected among rabbits and recently isolated from immunocompromised patients, suggesting zoonotic transmission. In this study, HEV infection among feral rabbits (Oryctolagus cuniculus) was assessed by detection of anti-HEV antibodies and HEV RNA. The prevalence of anti-HEV antibodies in sera was of 33 % (20/60) and HEV RNA was detected from only one of fecal swabs (1.7 %, 1/58). Furthermore, one naïve rabbit was intravenously inoculated with the suspension of the HEV-positive fecal specimen, exhibiting persistent HEV shedding in feces, intermittent viremia, seroconversion to anti-HEV IgM and IgG, and high alanine aminotransferase (ALT) values, indicating persistent HEV infection. The isolate JP-59 had a length of 7,282 bp excluding a poly (A) tail and possessed the characteristic 93 bp-insertion in ORF1. Phylogenetic analysis indicated that JP-59 formed a cluster with other rabbit HEV isolates from rabbits and human origin. The JP-59 shared the nucleotide sequence identities less than 87 % with other rabbit HEVs, suggesting that a novel rabbit HEV strain was circulating in Japan. [ABSTRACT FROM AUTHOR]

Genetic variation and differentiation in Magnolia stellata were studied in 20 populations distributed across most of the species' range using 10 microsatellite markers, and the factors influencing their levels of within-population genetic variation were examined. Generally, populations distributed intermittently from southern Gifu to central Aichi Prefectures showed substantially higher levels of genetic variation (exceptions included populations located at unusually high altitude sites or western and southern edges of the range) than more isolated populations on the Atsumi Peninsula of southern Aichi Prefecture and in northern Mie Prefecture. Significant isolation-by-distance patterns were detected in genetic differentiation among the studied populations, and a neighbor-joining tree based on DA distances among the populations reflected well the geographical positions of the populations. The level of within-population genetic variation was significantly influenced not only by the size of the populations (represented by the number of reproductive individuals) but also by their degree of isolation (represented by the number of populations within a radius of 0.5 km around them). Therefore, areas within radii of 0.5 km may encompass M. stellata metapopulations, in which gene flow may usually occur. We suggest that this area may be a suitable standard for constructing conservation units for the species.Heredity (2008) 100, 415–423; doi:10.1038/sj.hdy.6801097; published online 23 January 2008 [ABSTRACT FROM AUTHOR]

The prevalence of faecal carriage of salmonella in 5393 pigs reared on 218 pig farms located in 31 of 47 prefectures in Japan over the period July 2003 to June 2005 was investigated. We isolated 172 strains belonging to 20 serovars and one untypable Salmonella enterica from 169 pig faecal samples (3.1%) collected from 48 farms (22.0%). The most prevalent type of S. enterica was untypable O4,12:d:– which lacks phase 2 flagellar antigen, representing 29.1% (50/172) of all isolates. Of 26 S. enterica serovar Typhimurium isolates, 16 strains appeared to be definitive phage type 104 (DT104) by polymerase chain reaction. [ABSTRACT FROM AUTHOR]

y.-s. chen, f. yanagida and t. shinohara. 2005.To survey, and identify and classify the ecological distribution of lactic acid bacteria from soil in Japan and Taiwan.Acid-producing bacteria were isolated from 68 soil samples, collected from Japan and Taiwan, in the rhizospheres of fruit trees, from the floor of a henhouse and around a horse farm. All isolates were identified by physiological and genetic tests. Thirty-two of the 54 isolates were identified as lactic acid bacteria (LAB), 16 as spore-forming lactic acid bacteria, five asClostridiumand one asBacillus. These lactic acid bacteria represent five genera:Lactobacillus,Lactococcus,Enterococcus,LeuconostocandWeissella.A high rate of isolating lactic acid bacteria was obtained from soil.This study suggests that soil may be a common source for the isolation of lactic acid bacteria. [ABSTRACT FROM AUTHOR]

The Ryukyu-ayu, Plecoglossus altivelis ryukyuensis, is an amphidromous fish that is endemic to Amami-oshima Island in southernmost Japan. Its abundance, however, has been appreciably reduced during the last two decades such that the subspecies is now considered to be endangered. The variation of the mitochondrial DNA control region was investigated among specimens of the extant populations in the eastern and western parts of Amami-oshima Island (Sumiyo Bay and Yakeuchi Bay areas, respectively), using restriction fragment length polymorphism analysis in order to estimate the extent of gene flow between the two areas. Of a total of 165 fish including temporally different samples, four haplotypes were detected and each area possessed the two haplotypes. However, the common haplotypes shared between these areas were not observed, which indicates that recent gene flow has not occurred between these populations. The nucleotide divergence between populations was much higher than the nucleotide diversity within each population, and the neighbor-joining phylogram among haplotypes showed that the haplotypes are associated with their geographic area. These results suggest that the two populations of Ryukyu-ayu on Amami-oshima Island have been historically formed. [ABSTRACT FROM AUTHOR]

At present, there is scarce evidence about the burden associated with the isolation of COVID-19 patients. We aimed to assess the differences between COVID-19 and other influenza-like illnesses (ILIs) in disease burden brought by isolation. We conducted an online survey of 302 respondents who had COVID-19 or other ILIs and compared the burden of isolation due to sickness with one-to-one propensity score matching. The primary outcomes are the duration and productivity losses associated with isolation, the secondary outcome is the health-related quality of life (HRQoL) valuation on the day of the survey. Acute symptoms of outpatient COVID-19 and other ILIs lasted 17 (interquartile range (IQR) 9-32) and 7 (IQR 4-10) days, respectively. The length of isolation due to COVID-19 was 18 (IQR 10-33) days and that due to other ILIs was 7 (IQR 4-11) days, respectively. The monetary productivity loss of isolation due to COVID-19 was 1424.3 (IQR 825.6-2545.5) USD and that due to other ILIs was 606.1 (IQR 297.0-1090.9) USD, respectively. HRQoL at the time of the survey was lower in the COVID-19 group than in the 'other ILIs' group (0.89 and 0.96, P = 0.001). COVID-19 infection imposes a substantial disease burden, even in patients with non-severe disease. This burden is larger for COVID-19 than other ILIs, mainly because the required isolation period is longer.

This study assesses the gender differences in health and anxiety, especially pertaining to mental health problems and time-course effects. We surveyed 121 patients admitted to a hospital with a COVID-19 diagnosis between March 1 and August 31, 2020. Their mental status was evaluated on admission using the Japanese General Health Questionnaire-28 (GHQ-28) and State-Trait Anxiety Inventory-Form JYZ (STAI). The patients were divided into two groups depending on the period of prevalence, that is, the first and second waves of the pandemic in Japan (from the beginning of March to the end of May 2020, Time 1 = T1; and from the beginning of June to the end of August 2020, Time 2 = T2). A multivariate analysis of covariance revealed significant differences in gender by time interactions in the GHQ-28 subscale "Insomnia and anxiety" and STAI subscale "State-Anxiety." Post-hoc t -tests revealed that the scores of "Insomnia and Anxiety" and "State-Anxiety" were higher in women than in men at T1. However, no difference was observed at T2. Further, "Insomnia and Anxiety" and "State-Anxiety" were significantly higher at T1 than at T2 in female patients. There was no significant difference in males. Thus, female patients were more anxious and depressed in the early phase of the pandemic, whereas male patients had difficulties in coping with anxiety. We suggest more gender-specific mental care, particularly for women at the early stages of infection., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Tsukamoto, Kataoka, Mino, Ishibashi, Shibata, Matsuo and Fujiwara.)

Cats are an important host of Toxoplasma gondii from an epidemiological perspective because they are the only definitive hosts that excrete oocysts in their feces. In this study, 201 free-ranging cats in Okinawa were examined for T. gondii infection. Using the latex agglutination test, we detected antibodies against T. gondii in 26.9% (54/201) of the cats. Oocysts of T. gondii were not detected upon microscopic examination of the feces of 128 cats. T. gondii was isolated from the tissues of 9 out of 24 seropositive or pseudo-seropositive cats with a bioassay using laboratory mice. Genotyping for the GRA6 gene revealed that five and four of the isolates were type I and II, respectively.

Cryptococcus neoformans, the main pathogen in immunocompromised patients, is a ubiquitous free-living fungus that can be isolated from avian excreta, soils, and plant material. This study was carried out to determine the infection rate of pigeon lofts, Passeriformes, and Psittaciformes in Ahvaz, the capital of Khuzestan province in Iran and to determine varieties of Cryptococcus neoformans (C. neoformans). The 80 samples were collected from pigeon lofts. Also, 163 feces of captive birds (Passeriformes and Psittaciformes) which kept in Ahvaz pet shops, and the 70 cloacal swabs of pet birds (Passeriformes and psittaciformes) referring to the department of avian medicine (the faculty of veterinary medicine of Shahid Chamran University of Ahvaz) were analyzed. The samples were directly inoculated on niger seed agar (NSA) and also enriched in brain heart infusion broth and then inoculated on NSA. Dark brown colonies suspected to C. neoformans subcultured on saborouds dextrose agar and pure cultures subjected to molecular (polymerase chain reaction (PCR)) diagnosis. For detection of C. neoformans, primer sets that targeting the CNLAC1 gene were selected and nested PCR was conducted. For identification of C. neoformans varieties, a primer set targeting the STR1 gene was selected. For more accurate confirmation, the purified PCR products of some isolates were also sequenced, and based on the gene sequences, all of the isolates belonged to C. neoformans variety grubii (var. grubii)(serotype A). Totally 16 out of 80 pigeon samples (20%) were contaminated with C. neoformans. The results in pigeons disclosed a 98.64% identity when compared with other strains of C. neoformans (CN1525, T4, and T1) which were previously deposited in GenBank from Italy and Thailand. Also, 21 out of 233 samples from Psittaciformes (9.01%) were contaminated with C. neoformans. The results in Psittaciformes disclosed a 99.7% identity when compared with other strains of C. neoformans (TIMM1313, IFM5882, CN1525, etc.) which were previously deposited in GenBank from Japan and Italy, etc. In the present study, the samples belonging to the passerine order were free of C. neoformans infection. According to the results, C. neoformans is prevalent in pigeon flocks and pet birds including Psittaciformes in the Ahvaz area, and should be considered by pigeon and captive bird breeders, veterinarians, and public health organizations in Ahvaz. The cryptococcus species isolated from captive birds and pigeons could be potential pathogens in humans., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Abstract: Bartonella henselae, the causative agent of cat scratch disease, is increasingly recognized as a cause of culture-negative endocarditis. This report describes the first Japanese case, which was diagnosed after a prolonged culture of the excised aortic valve. High IgG and IgM titers to B. henselae pointed to a subacute course of the disease. [Copyright &y& Elsevier]

We investigate the age structured data for the COVID-19 outbreak in Japan. We consider a mathematical model for the epidemic with unreported infectious patient with and without age structure. In particular, we build a new mathematical model and a new computational method to fit the data by using age classes dependent exponential growth at the early stage of the epidemic. This allows to take into account differences in the response of patients to the disease according to their age. This model also allows for a heterogeneous response of the population to the social distancing measures taken by the local government. We fit this model to the observed data and obtain a snapshot of the effective transmissions occurring inside the population at different times, which indicates where and among whom the disease propagates after the start of public mitigation measures. [ABSTRACT FROM AUTHOR]

• In vitro isolation and genotyping of two new Neospora caninum isolates is described. • Nc-Spain11 and Nc-Spain12 were isolated from two congenitally infected lambs. • Neospora caninum ovine isolates were implicated in reproductive failure in a flock. • Both isolates showed identical multilocus microsatellite genotype. • Ovine genotype showed close genetic relationship with bovine genotypes. Neospora caninum is considered one of the main causes of abortion in cattle but can also cause abortion in sheep. There is limited knowledge of the N. caninum population infecting sheep, and only one N. caninum isolate from a pregnant sheep from Japan has been reported. This study describes the in vitro isolation and genetic characterization of two new sheep isolates of N. caninum implicated in ovine reproductive failure. We used IFN-γ-knockout mice inoculated with PCR-positive brain homogenates from two clinically healthy but congenitally infected lambs at 4.5 months of age for parasite isolation. The lambs were born to dams from a sheep farm that had experienced pregnancy failure caused by N. caninum in successive generations. Tachyzoites were microscopically visualized in peritoneal flushes from all inoculated mice and were also observed in MARC-145 cell cultures within one week after inoculation with peritoneal flushes. Two N. caninum isolates, Nc-Spain11 and Nc-Spain12, were obtained from each lamb. The genotyping of the Nc-Spain11 and Nc-Spain12 isolates based on 9 microsatellite markers showed identical multilocus genotype (MLG). Comparison between a previous N. caninum genotype dataset including 80 MLGs from Argentinean, Spanish, Mexican, German and Scottish bovine isolates and the Japanese sheep isolate showed that the Nc-Spain11 and Nc-Spain12 MLG was unique and differed from the other MLGs. eBURST analyses showed that the Nc-Spain11 and Nc-Spain12 MLG was genetically clustered with other bovine MLGs and one ovine MLG, and the nearest genetic relationship was with an MLG from a bovine abortion collected in the same geographical area of Galicia. [ABSTRACT FROM AUTHOR]

Seven hyphomycete isolates from forest environments of the Ryukyu Islands, Japan, were identified toGliocephalotrichum microchlamydosporumandG. simplex. The former species is a new record in Japan. Although the five known species in the genusGliocephalotrichumincluding two isolates ofG. microchlamydosporumand four ofG. simplexin this study are all soilborne, another isolate ofG. simplexwas first isolated from an unidentified basidiocarp tissue. [ABSTRACT FROM AUTHOR]