1. Lung Mucosa Lining Fluid Modification of Mycobacterium tuberculosis to Reprogram Human Neutrophil Killing Mechanisms.
- Author
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Tomoki Yoshikawa, Masayuki Shimojima, Shuetsu Fukushi, Hideki Tani, Aiko Fukuma, Satoshi Taniguchi, Harpal Singh, Yuto Suda, Komei Shirabe, Shoichi Toda, Yukie Shimazu, Taro Nomachi, Mutsuyo Gokuden, Toshiharu Morimitsu, Katsuyuki Ando, Akira Yoshikawa, Miki Kan, Marina Uramoto, Hideo Osako, and Kouji Kida
- Subjects
THROMBOCYTOPENIA ,TICK-borne diseases ,PHYLOGENY ,FEVER ,MOLECULAR epidemiology - Abstract
We have shown that human alveolar lining fluid (ALF) contains homeostatic hydrolases capable of altering the Mycobacterium tuberculosis cell wall and subsequently its interaction with human macrophages. Neutrophils are also an integral part of the host immune response to M. tuberculosis infection. Here we show that the human lung mucosa influences M. tuberculosis interaction with neutrophils, enhancing the intracellular killing of ALFexposed M. tuberculosis and up-regulating the expression of tumor necrosis factor and interleukin 8. In contrast, ALF-exposed M. tuberculosis does not induce neutrophil apoptosis or necrosis, degranulation, or release of extracellular traps, and it decreases the oxidative response. These results suggest an important role for the human alveolar mucosa: increasing the innate capacity of the neutrophil to recognize and kill M. tuberculosis by favoring the use of intracellular mechanisms, while at the same time limiting neutrophil extracellular inflammatory responses to minimize their associated tissue damage. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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