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Forensic genetics is a rapidly evolving science thanks to the growing variety of genetic markers, the establishment of faster, less error-prone sequencing technologies, and the engineering of bioinformatics models, methods, and structures. In the early 2000s, the need emerged to create an international genetic database for forensic purposes. This paper describes a judicial investigation of skeletal remains to identify the subject using various methods. The anthropological examination of the remains allowed identification of the Caucasoid (European) ethnic group, a height of 156 ± 4 cm, and an age between 47 and 50 years. The genetic profiles obtained from typing several microsatellites made it possible to evaluate the compatibility between the skeletal remains and the suspected decedent. To identify the remains, the two extrapolated genetic profiles were compared. The case described highlights the central role of forensic genetics in identifying skeleton remains by means of comparison. [ABSTRACT FROM AUTHOR]

Background: Italy was declared malaria free by the World Health Organization in 1970. Despite this, nonimport malaria cases are on the increase in Italy and throughout the Mediterranean area. In Italy, in the period between 2011 and 2015, seven cases of locally acquired malaria have been reported, including one introduced case of Plasmodium vivax; moreover, the last certain case of introduced malaria (by P. vivax) has been reported in Tuscany in 1997. No case of introduced malaria from Plasmodium falciparum has been reported in Italy since 1970. Case Presentation: A cluster of four cryptic P. falciparum malaria cases were ascertained in migrant farm workers (three from Morocco and one from Sudan) in Apulia (southern Italy) with clinical onset between September 20 and 27, 2017. None of the patients reported a history of a recent trip to malaria-endemic areas or hospitalization or other risk factors. Typing of malaria was also confirmed using molecular biology methods in two different laboratories. There were no cases of severe malaria in our four patients, and only one in need of transfusion. All patients were discharged cured after being treated with mefloquine due to the unavailability of other antimalarials. Conclusions: In recent years, numerous reports of locally acquired malaria have been made in southern Europe. The cases described in this article represent the first cluster of malaria caused by P. falciparum in Europe. Today, clinical presentation in the diagnosis of malaria is more important than ever, since epidemiological criterion cannot be considered unfailing. The mode of transmission has not been proven and further biological and entomological studies are necessary to define our case as cryptic or confirm the presence of mosquitoes capable of transmitting P. falciparum and/or the capacity of Anopheles labranchiae, An. superpictus, or An. plumbeus to transmit it on Italian territory. [ABSTRACT FROM AUTHOR]

Abstract: The authors describe the discovery of the remains of two unidentified skeletonised individuals in a small town located in southern Italy. The bodies were discovered while workers were preparing to lay an oil pipeline. The two individuals were found at a depth of 2 meters, and in very close proximity to one other. The recovery process of the skeletonised remains and their related findings, carried out by a team of forensic anthropologists and archaeologists, is described here. Archaeological examination determined that the remains date back to the 4th millennium B.C. Forensic anthropological and odontological examinations were performed to determine the biological profile of the skeletal remains by estimation of age and height, as well as the determination of sex. Age determination was performed by the Kerley and Ubelaker (1978, Revision in the microscopic method of estimating age at death in human cortical bone. Am. J. Phys. Anthropol., 49, 545–546) histological method using a 1mm thick piece of bone tissue taken from the diaphyses of femur in both individuals. Dental age was estimated by examining root transparency. In order to determine the height of the individuals, various research methods based on the dimensional values of particular skeletal structures were applied. DNA analysis showed genotype differences of all the systems as compared to the haplotypes of present day subjects. This provided confirmation that the skeletal remains were from individuals of an ancient population (4th millennium B.C.). In addition, radiocarbon dating provided useful information as to the approximate period of death of the individuals. Interpretation was further enhanced by analysis of various bone fragments from each of the skeletons by high resolution mass spectrometry. 3D computerized imaging was used to analyse the patterns of skull fractures present, which resulted in supporting the hypothesis that the fractures were caused by stoning. [Copyright &y& Elsevier]