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In the main strawberry areas of Southern Italy, cultivation is carried out by transplanting plants on raised beds (30–40 cm from ground level), mulched with black polyethylene (PE). This technique has becoming increasingly expensive due to the growing prices of plastic mulches, the cost to remove them at the end of crop cycle, and the difficulty to dispose of black, dirty plastic films. The main objective of this research was the replacement of PE mulch with a new biodegradable mulching film Mater-Bi®-based (Novamont), characterized by an increased permanence in the field designed for long crop life. In 2021, two Mater-Bi-based, black, 18 μm thick mulching films were tested under tunnel: N5 as innovative film and N18 as commercial standard film. Black PE film, 50 μm thick was the control. Strawberry cultivars 'Sabrina' and 'Elide' were cropped on the three mulching films according to a split plot design with four replications. Harvests lasted from March to June 2021. Cvs Sabrina and Elide yielded around 40 t ha−1, while the mean effect of mulching films did not point out differences between the biodegradable mulches and PE. In 4 out of 12 harvests we analyzed samples of fruits to assess the influence of mulches on the contents of °Brix, polyphenols, antioxidant activity, ascorbic acid, flavonoids, and anthocyanins. On average, °Brix was clearly improved in fruits on PE compared to biodegradable films, while all the other qualitative traits resulted in being more dependent on the cultivars and times of sampling effects. Overall, biodegradable mulches are a viable alternative to PE mulch, and the innovative N5 film appeared promising for the enhancement of durability of soil coverage in a long-lasting cycle. [ABSTRACT FROM AUTHOR]

The Annurca apple is a typical fruit from Southern Italy that is notable for its high firmness and characteristic flavor, and presents positive effects on human health. The aim of the present study was to improve the agronomic characteristics of Annurca trees and the quality of their fruits by the use of plant biostimulants. We performed the study with three biostimulants (protein hydrolysates, seaweed extracts, and microalgae) to determine their effects on trees and fruits at harvest and during fridge conservation. The measurements in the field concerned the production per plant, the number of fruits, the diameter and fruit weight, the diameter of trunk, the weight of pruning, the first harvest %, and yield efficiency (plant prod./TCSA) for both growing seasons 2018–2019. The analyzed fruit parameters at harvest and during the fridge conservation were: flesh firmness, epicarp color, total soluble solids, pH, and titratable acidity. The results showed positive effects of the biostimulants on the production and the color parameters, especially with microalgae enhancing fruit redness (5-fold) and color index (8.5-fold). In particular, protein hydrolysates and microalgae increased the production (10.4%), diameter of the fruits (7.0%), the first harvest percentage (37.8%), and yield efficiency (6.9%). The tested plant biostimulants showed no significant effect on the measured qualitative parameters. This study represents the first detailed research on the use of different types of biostimulants on Annurca trees and their impact on the quality of its fruits from harvest to storage, and may present a sustainable alternative for the "melaio" processes. [ABSTRACT FROM AUTHOR]

The cultivar Annurca is an apple that is cultivated in southern Italy that undergoes a typical redding treatment and it is appreciated for organoleptic characteristics, high pulp firmness, and nutritional profile. In this study, the effects of three different biostimulants (Micro-algae (MA), Protein hydrolysate (PEP), and Macro-algae mixed with zinc and potassium (LG)), with foliar application, on the quality parameters of Annurca apple fruits at the harvest, after redding, and at +60 and +120 days of cold storage were analyzed: total soluble solids (TSS) content, total acidity (TA), pH, firmness flesh, and red coloration of epicarp. Additionally, the polyphenolic quali-quantitative profile of pulp and peel was analyzed by UHPLC-Q-Orbitrap HRMS and Folin-Ciocalteu and the antioxidant capacity with the methods 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric-reducing antioxidant capacity (FRAP). The results obtained suggest that biostimulants are involved in the regulation of the secondary metabolism of the treated plants, acting positively on the quality of the Annurca fruits and their nutritional value. Fruits treated with PEP have shown, during cold storage, a significantly higher content of total polyphenols in flesh and a higher concentration of phloretin xylo-glucoside and phloridzin (350.53 and 43.58 mg/kg dw respectively). MA treatment caused, at the same time, an enhancement of flavonols between 0.6–28% and showed the highest total polyphenol content in the peel after 60 and 120 days of cold storage, with 2696.048 and 2570.457 mg/kg dw, respectively. The long-term cold storage (120 days) satisfactorily maintained phenolic content of fruits deriving from MA and PEP application, in accordance with data that were obtained for peel, showed an increase of 7.8 and 5.8%, respectively, when compared to the fruits cold stored for 60 days. This study represents the first detailed research on the use of different types of biostimulants on the quality of the Annurca apple from harvest to storage. [ABSTRACT FROM AUTHOR]