1. Phytochemical profile and α-glucosidase inhibitory activity of Sardinian Hypericum scruglii and Hypericum hircinum.
- Author
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Mandrone, Manuela, Scognamiglio, Monica, Fiorentino, Antonio, Sanna, Cinzia, Cornioli, Luca, Antognoni, Fabiana, Bonvicini, Francesca, and Poli, Ferruccio
- Subjects
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ALTERNATIVE medicine , *ANTIOXIDANTS , *DOSE-effect relationship in pharmacology , *ENZYME inhibitors , *HIGH performance liquid chromatography , *HYPERICUM perforatum , *LIQUID chromatography , *MASS spectrometry , *MEDICINAL plants , *NUCLEAR magnetic resonance spectroscopy , *PHYTOCHEMICALS , *PLANT extracts , *IN vitro studies , *PHARMACODYNAMICS - Abstract
The phytochemical profile, antioxidant activity and α-glucosidase inhibitory activity of Hypericum scruglii Bacchetta, Brullo et Salmeri and Hypericum hircinum L. were analyzed and compared to those of the best-known Hypericum perforatum L. Samples were prepared using three different extraction methods (maceration in 70% ethanol, extraction in MeOH/phosphate buffer and decoction), the influence of which on the metabolic profile and bioactivities are discussed. H . scruglii and H . hircinum extracted in 70% ethanol strongly inhibited α-glucosidase (IC 50 7.25 and 14.05 μg/ml, respectively), which is a valuable enzymatic target for treating metabolic disorders, while H . perforatum was found to be less powerful than the other two species in all the performed biological tests. The phytochemical profile was analyzed by NMR, HPLC-DAD and HPLC-FLD, revealing remarkable differences among the species. In particular, H . scruglii , which is a species endemic to Sardinia Island (Italy), was particularly enriched in two phloroglucinol-derivatives identified by means of 2D NMR and LC/MS/MS experiments as 3-geranyl-1-(2′-methylbutanoyl)-phloroglucinol and 3-geranyl-1-(2′-methylpropanoyl)-phloroglucinol. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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