1. ISOLATION AND MOLECULAR IDENTIFICATION OF THE FUSARIUM HEAD BLIGHT SPECIES ON WHEAT IN AL-SHIRQAT DISTRICT, IRAQ.
- Author
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Akram, Salim I., Al-Taae, Ali K., and Thalij, Karkaz M.
- Subjects
FUSARIUM ,WHEAT ,DNA primers ,RIBOSOMAL DNA ,NUCLEOTIDE sequence ,IDENTIFICATION ,ROOT rots - Abstract
The current work was conducted on Fusarium head blight disease of wheat that was caused by Fusarium species. A survey was carried out of several wheat fields at AL-Shirqat district, Saladin Governorate during two growth periods of wheat (Booting to Heading) From the middle of April to May and (Ripening) the second phase of the period from May to the first of June during the growing season 2019-2020. The highest disease incidence on both stages was 33.136 and 51.002%, respectively. The lowest disease incidence in the first period was recorded in 2.936% and increased to 4.18% in the second period. Fusarium graminearum, were isolated from root, crown and spikes of wheat It was isolated from spikes in high frequency at 34.208%. Fusarium spp. were isolated from all wheat fields in both stages. Pathogenicity of the fungus in wheat plants was also confirmed by Koch's postulates. Molecular identification of Fusarium isolates was done by amplifying the internal transcribed spacer ITS region of the conserved ribosomal DNA using primers ITS1 and ITS4. All the ITS sequences were compared for gaps and similarity sequences of the fungus were homologous to those of F. graminearum isolates in the GenBank database with a similarity percentage of 99%, thereby confirming the identity of the causative agent of the disease. This could be the first recording of F. graminearum on the wheat in AL-Shirqat district Saladin Governorate, Iraq. The nucleotide sequence of ITS from the Iraqi isolate has been assigned ýGenBank Accession No MW737662.1. [ABSTRACT FROM AUTHOR]
- Published
- 2022