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The purpose of this study was to determine the effect of landmarks on learning basketball lay-up in beginners. Twenty-seven females (age = 20.30 ± 0.24 years, height = 164.37 ± 0.53 cm) participated in this study. They were assigned to three groups: no landmark group, colored landmark group, and black and white landmark group. All participants performed basketball lay-up for six sessions. The colored group and black and white group trained with the landmarks on the ground while the no landmark group trained without any landmarks based on a trainer instruction. Posttest was performed at the end of the last session of training and also in the retention test following 72 hr. All trials were assessed through 5-point Likert scales, ranging from 1 (very poor) to 5 (very good). The results showed that using landmarks significantly changed the lay-up pattern; however, these changes were not significant for color and lay-up shot accuracy.