1. Inhibitory Effect of Hemiscorpius lepturus Scorpion Venom Fractions on Tachyzoites of Toxoplasma gondii.
- Author
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Rostamkolaie, Lida Khaleghi, Hamidinejat, Hossein, Razi Jalali, Mohammad Hossein, Varzi, Hossein Najafzadeh, Seifi Abadshapouri, Massoud Reza, and Jafari, Hedieh
- Subjects
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SCORPION venom , *SCORPIONS , *TOXOPLASMA gondii , *GEL permeation chromatography , *SNAKE venom , *HIGH performance liquid chromatography , *TRYPAN blue , *PEPTIDES - Abstract
Background: The present study determined the effect of the fractions obtained from Hemiscorpius lepturus scorpion venom on the tachyzoite of Toxoplasma gondii. Methods: The fractions of dried venom of He. lepturus scorpion of Khuzestan Province, southern Iran in 2019 were isolated through gel filtration chromatography, and then tachyzoites were exposed to fractions of venom at different concentrations. Trypan blue counting and MTT were applied to assay tachyzoite viability, and the inhibition of the cellular growth of fractions in Vero cells was evaluated. Results: The maximum effect on tachyzoite was observed in fraction 5 of venom. To further separate the protein, fraction 5 was used in high-performance liquid chromatography assay to purify its proteins. Based on the results of HPLC of fraction 5, among which the second peak, a peptide with <10 IKDa representing a more potent effect in eliminating the tachyzoite of T. gondii. Conclusion: The scorpion venom-purified fractions possess anti-parasitic activity against the tachyzoite of T. gondii and can be used in parasite-controlling studies. [ABSTRACT FROM AUTHOR]
- Published
- 2022