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In this study, 10 different traditional Iranian cheeses, which are still consumed by people in rural areas of Iran, were examined to isolate new strains of probiotic bacteria. Isolated bacteria were identified by 16s rRNA gene amplification and subjected to series of in vitro tests to find out their probiotic potential. A total of 2345 colonies were collected and 465 of them were confirmed as lactic acid bacteria (LAB), of which Lactiplantibacillus plantarum, Lactobacillus bulgaricus, and Lacticaseibacillus casei were the top three isolated bacteria. Among the different species of LAB isolated in this study, Lactip. plantarum was the most isolated species, and seven isolates had the significant criteria for being a probiotic strain than other isolates indicating the most adaptable properties of this species. Lactiplantibacillus plantarum was the most resistant bacteria in the bile resistance test and was also the most durable bacteria in gastrointestinal conditions, for example, acidic environment (pH = 2.5) and trypsin. In contrast, Lacticaseibacillus casei was the most susceptible bacterial strain. Lactobacillus rhamnosus showed the most antibacterial effect against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. This study showed that probiotic strains isolated from local cheeses could be considered as suitable biopreservatives and used as specific starter cultures for the production of functional cheeses. [ABSTRACT FROM AUTHOR]

Background and Aims: Colorectal cancer is considered to be an important cause of morbidity and mortality worldwide. Anti-cancer properties of probiotic lactic acid bacteria are considerable for treatment of cancers such as colorectal cancer. The aim of this study was to investigate the molecular characterization of probiotic bacterial isolates from collected stool specimens in west of Mazandaran province, in order to confirm the probiotic effect of isolated strains, so to compare their effect on cancer cell lineages, in future studies. Materials and Methods: In this study, out of 60 stool samples collected from healthy children and adult people, 50 bacterial strains were isolated. Antibiotic susceptibility was performed by 15 antibiotics for 7 strains. Then, pH and bile salts tolerance were measured in the strains. Molecular analysis of these strains was carried out by PCR-sequencing and their results were used to draw a phylogenetic tree. Results: Of the 50 isolates, 7 isolates were lactic acid bacteria and two strains were completely probiotic. These strains were resistance to low pH and 0.3% bile salt. Molecular analysis showed that two strains SE and SG were probiotics related to Lactobacillus fermentum (86% homology) and Lactobacillus rhamnosus (61% homology) family, respectively, which were known as new strains. Conclusion: The results suggested that there are 7 strains with good tolerance in the gastrointestinal tract that have probiotic properties and this is the basis of future studies to evaluate their anti-tumor properties and ultimately oral administration. [ABSTRACT FROM AUTHOR]

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