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Abstract: The subject of the present study is the evaluation of the concentrations of mercury (Hg) and selenium (Se) in fish and shellfish from the Gulf of Catania (Ionian Sea) and the assessment of related risk-based consumption limits per single contaminant in adults and children. In contrast to the potential harm from Hg, Se is an essential element that is normally found in high levels in seafood. If the amount of Hg is high enough, it could bind Se and irreversibly inhibit selenium-dependent enzymes. Thus, adequate levels of Se need to be available to replace the amount of Se lost to Hg sequestration, thereby maintaining normal selenoprotein synthesis. Hg analysis was conducted using a flow injection analysis system coupled with an atomic adsorption spectrometer, and Se analysis was conducted using an inductively coupled plasma mass spectrometry (ICP-MS). Of the trace elements investigated, only Hg has a limit set by the European Community for human consumption, and this was never exceeded. Nevertheless, based on Target Hazard Quotient (THQ) over 1, and on the Estimated Daily Intake per meal (EDIm) higher than the Provisional Tolerable Intake (PTI) suggested by the Joint FAO/WHO Expert Committee on Food Additive (JECFA), Hg oral exposure derived from consumption of the benthonic fish and of the bigger pelagic fish species analyzed, could follow the occurrence of systemic effects. Se was found always in molar excess respect to Hg in all pelagic fish and in the shellfish, nearly equimolar in the benthonic fish. Determining the evidence that foods, such as pelagic fish, with high molar excess of Se, could contribute to replace the amount of Se bound to Hg and thereby maintaining normal selenoprotein synthesis, is useful for a better understanding of the seafood safety. [Copyright &y& Elsevier]

Abstract: In the last years, the development of new techniques and the increase of sophisticated assays has improved the environmental monitoring programs by using fish as bioindicators of marine ecosystems, applying a multibiomarkers approach. The aim of this study was to assess the contamination level of the Catania Gulf through bioaccumulation and contaminant exposure response in Engraulis encrasicolus (Linnaeus, 1758). Heavy metals, polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) were analyzed in muscle tissue of the species. Furthermore we evaluated biomarkers of exposure to such contaminants: the metallothioneins (MTs), the ethoxyresorufin-O-deethylase (EROD) and stress protein or “heat shock proteins” (HSP70). In total, three sampling of 30 specimens each were performed during 2011. Comparison between classes of contaminants was performed using Student''s t-test for paired samples Data showed that: the populations of E. encrasicolus accumulated a certain amount of metals, PAHs and organochlorine compounds in their tissues, that, however, did not exceed the legal limits set by Regulation 1881/2006 for contaminants in fish muscle; essential metals accumulate more during the reproductive periods of the species; the accumulation of heavy metals and PAHs are more closely related to natural and anthropogenic events; the presence of PCBs, although in traces, is the confirmation that banned compounds persist in the environment for several years; the levels of contaminants found in the tissues of the species do not seem to be likely to cause toxic effects both in the studied fish species. In conclusion, environmental risk assessment came out in this study for the Catania Gulf, showed a positive picture, although the contaminants detected are related to a certain degree of anthropic impact, and should be constantly monitored to ensure quality standards of the studied area. [Copyright &y& Elsevier]