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Apple geminivirus 1 (AGV) in the genus Maldovirus of the family Geminiviridae was first identified infecting apple trees in the year 2015 in China. In this work, we characterized three isolates of the AGV in the Chinese pearleaf crabapple (Malus asiatica) in Inner Mongolia Autonomous Region. The viruses were detected by Illumina sequencing and its existence was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) amplification of an AGV fragment. Between the three AGV isolates and the initially characterized AGV isolate PL2015, the nucleotide sequence identities of the complete genome ranged from 91.2 to 91.7%, of the coat protein gene (V1) ranged from 95.4% to 97.3%, and of the replicase gene (C1) ranged from 87.3% to 88.0%. Phylogenetic analysis indicated that the three isolates formed a monophyletic group together with the AGV, separated from the current genera in the family Geminiviridae. This is the first description of the AGV infecting crabapples. [ABSTRACT FROM AUTHOR]

Prunus necrotic ringspot virus (PNRSV) is an important virus of stone fruit trees (Prunus sp.). In this study, complete genomic sequences of five PNRSV isolates from P. persica (Pea1 and Pea4), P. avium (Che1 and Che2) and P. cerasifera (P.cerasifera1) in Inner Mongolia of China were determined and analyzed together with other previously published PNRSV sequences in the world. Phylogenetic analysis based on RNA3 sequences divided the five isolates into PV32 (Che1, Che2 and P.cerasifera1) and PV96 (Pea1 and Pea4) group, and re-grouped PNRSV isolates in the world into six groups including a new proposed group (PchMx-Azt) composed of two flowering peach isolates from Mexico. Distinct origins for RNAs of isolate P.cerasifera1 suggested potential reassortment in evolution of PNRSV. In addition, nine recombination events were predicted in RNA1–3 among the isolates. [ABSTRACT FROM AUTHOR]