1. Regulatory T cells in human geohelminth infection suppress immune responses to BCG and Plasmodium falciparum.
- Author
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Wammes LJ, Hamid F, Wiria AE, de Gier B, Sartono E, Maizels RM, Luty AJ, Fillié Y, Brice GT, Supali T, Smits HH, and Yazdanbakhsh M
- Subjects
- BCG Vaccine immunology, BCG Vaccine pharmacology, CD4-Positive T-Lymphocytes cytology, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes metabolism, Cell Proliferation drug effects, Cells, Cultured, Child, Erythrocytes immunology, Erythrocytes parasitology, Helminthiasis parasitology, Humans, Indonesia, Interferon-gamma metabolism, Interleukin-2 Receptor alpha Subunit metabolism, Leukocytes, Mononuclear cytology, Leukocytes, Mononuclear immunology, Leukocytes, Mononuclear metabolism, Lymphocyte Count, Malaria, Falciparum microbiology, Plasmodium falciparum growth & development, Plasmodium falciparum immunology, T-Lymphocytes, Regulatory cytology, T-Lymphocytes, Regulatory metabolism, Tuberculosis microbiology, Helminthiasis immunology, Malaria, Falciparum immunology, T-Lymphocytes, Regulatory immunology, Tuberculosis immunology
- Abstract
Chronic helminth infections induce T-cell hyporesponsiveness, which may affect immune responses to other pathogens or to vaccines. This study investigates the influence of Treg activity on proliferation and cytokine responses to BCG and Plasmodium falciparum-parasitized RBC in Indonesian schoolchildren. Geohelminth-infected children's in vitro T-cell proliferation to either BCG or pRBC was reduced compared to that of uninfected children. Although the frequency of CD4(+)CD25(hi)FOXP3(+) T cells was similar regardless of infection status, the suppressive activity differed between geohelminth-infected and geohelminth-uninfected groups: Ag-specific proliferative responses increased upon CD4(+)CD25(hi) T-cell depletion in geohelminth-infected subjects only. In addition, IFN-gamma production in response to both BCG and parasitized RBC was increased after removal of CD4(+)CD25(hi) T cells. These data demonstrate that geohelminth-associated Treg influence immune responses to bystander Ag of mycobacteria and plasmodia. Geohelminth-induced immune modulation may have important consequences for co-endemic infections and vaccine trials.
- Published
- 2010
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