1. Mechanism-based CYP2D6 inactivation by acridone alkaloids of Indonesian medicinal plant Lunasia amara
- Author
-
Takahashi, Naoto, Subehan, Kadota, Shigetoshi, and Tezuka, Yasuhiro
- Subjects
- *
MEDICINAL plants , *ALKALOIDS , *ALTERNATIVE medicine , *ANALYSIS of variance , *BIOLOGICAL models , *BIOPHYSICS , *PHYSICAL & theoretical chemistry , *ENZYME inhibitors , *RESEARCH methodology , *NUCLEAR magnetic resonance spectroscopy , *REGRESSION analysis , *T-test (Statistics) , *THIN layer chromatography , *PLANT extracts , *DATA analysis software , *DESCRIPTIVE statistics , *PHARMACODYNAMICS - Abstract
Abstract: Fourteen acridone alkaloids isolated from Lunasia amara Blanco were tested for their mechanism-based inhibition on human liver microsomal dextromethorphan O-demethylation activity, a prototype marker for cytochrome P450 2D6 (CYP2D6). Among the 14 compounds, 5-hydroxygraveroline (1), 8-methoxyifflaiamine (2), lunamarine (3), and lunine (12) increased their inhibitory activity with increasing preincubation time. Then, we further examined the possibility of mechanism-based inhibition on 5-hydroxygraveroline (1) and lunamarine (3), which showed the potent inhibition. Further investigations on 1 and 3 showed that the characteristic time- and concentration-dependent inhibition, which required a catalytic step with NADPH, was not protected by nucleophiles, and was decreased by the presence of a competitive inhibitor. Thus, 1 and 3 were concluded as mechanism-based inactivators of CYP2D6. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF