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This study described the implementation of STEM-based science learning and performance assessment to the improvement of students' thinking skills in Aceh, Indonesia. The purposive sampling technique was employed in this descriptive research. The respondents were 300 students at grade 11th academic year 2019/2020, representing five public high schools in Aceh, Indonesia. The instruments used to measure the effectiveness of the implementation of STEM-based science learning were the rubric of habits of mind, observation sheets of performance assessments, and questionnaires. The finding presented the implementation of STEM-based science learning was able to habituate and develop students' habits of mind with an average score of 0.71 which included into high category. The average value of the overall STEM-based science learning implementation for the formation of habits of mind is 95.98% which categorized as good. It can be concluded that there is the success of researchers in implementing STEM-based science learning to form students' habits of mind.

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<b>Background and Objective:</b> The existence of intensive shrimp aquaculture faces serious challenges in the form of a decrease in pond water quality due to overfeeding. Efforts are needed to improve pond water quality by utilizing proteolytic bacterial isolates to break down suspended or accumulated feed on the pond bottom. The research aims to find proteolytic bacterial isolates from pond sediments and the digestive tract of shrimp (<i>Litopenaeus vannamei</i>). <b>Materials and Methods:</b> The materials needed are pond sediment samples, shrimp digestive tract, seawater complete agar (SWCA) medium and skim milk agar medium (SMA). The study used survey methods to determine sampling locations and continued with experimental methods in the laboratory. Nine isolates were obtained from pond sediments and two bacterial isolates were from the shrimp digestive tract. <b>Results:</b> The proteolytic potency test showed that two isolates from pond sediments and one isolate from the digestive tract of shrimp were positive for proteolytic. The largest proteolytic index value reached 6.357. Molecular identification by analyzing the <i>16S rRNA</i> gene sequence shows that PC23 isolate is closely related to the bacterium <i>Exiguobacterium indicum </i>strain KR6 with percent identity 99.44-99.58% and PU32 isolate with <i>Bacillus cereus</i> strain 125 with percent identity 100%. <b>Conclusion:</b> The bacteria obtained can be used as probiotic candidates for the future are <i>Exiguobacterium indicum</i> strain KR6 and <i>Bacillus cereus</i> strain 125.

The government of Serdang Bedagai Regency initiated a supplementation program to reduce the high prevalence of stunting in the area by delivering extra supplementation, which were nutritious biscuits from national government and fish-based supplement produced from local resources. A 6-month study from April 2022 to September 2022 was conducted to monitor and evaluate the government program that involved 219 under-5-year-old children with height-for-age Z-score (HAZ-score) below - 2. We observed the stunting prevalence reduction by 37.00%, where 81 children recovered from stunting (HAZ-score ≥ - 2). Furthermore, the mean HAZ-score and WHZ-score (Weight-for-Height Z-score) were monitored to significantly improve by 0.97 ± 1.45 (P-value = 1.74e -14 ) and 1.00 ± 2.18 (P-value  = and 2.40e -8 ), subsequently. The most significant improvement in HAZ-score was monitored among children receiving fish-based supplements with 1.04 ± 1.44 improvement (P-value = 6.59e -17 ). Then, a significant WHZ-score improvement was reported from children consuming fish-based supplements and a combination of fish-based supplements with nutritious biscuits (P-value = 2.32e -8 and 5.48e -5 ) by 1.04 ± 2.29 and 0.83 ± 1.84, respectively. The results of the observation become evidence that the program could effectively reduce the prevalence of stunting in children below five years old, especially among children who received locally produced fish-based supplements., (© 2024. The Author(s).)

Background: Bio Farma has developed a recombinant protein subunit vaccine (IndoVac) that is indicated for active immunization in population of all ages. This article reported the results of the phase 3 immunogenicity and safety study in Indonesian adults aged 18 years and above., Methods: We conducted a randomized, active-controlled, multicenter, prospective intervention study to evaluate the immunogenicity and safety of IndoVac in adults aged 18 years and above. Participants who were SARS-CoV-2 vaccine-naïve received two doses of either IndoVac or control (Covovax) with 28 days interval between doses and were followed up until 12 months after complete vaccination., Results: A total of 4050 participants were enrolled from June to August 2022 and received at least one dose of vaccine. The geometric mean ratio (GMR) of neutralizing antibody at 14 days after the second dose was 1.01 (95 % confidence interval (CI) 0.89-1.16), which met the WHO non-inferiority criteria for immunobridging (95 % CI lower bound > 0.67). The antibody levels were maintained through 12 months after the second dose. The incidence rate of adverse events (AEs) were 27.95 % in IndoVac group and 32.15 % in Covovax group with mostly mild intensity (27.70 %). The most reported solicited AEs were pain (14.69 %) followed by myalgia (7.48 %) and fatigue (6.77 %). Unsolicited AEs varied, with each of the incidence rate under 5 %. There were no serious AEs assessed as possibly, probably, or likely related to vaccine., Conclusions: IndoVac in adults showed favourable safety profile and elicited non-inferior immune response to Covovax. (ClinicalTrials.gov: NCT05433285, Indonesian Clinical Research Registry: INA-R5752S9)., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Asrawati Nurdin reports financial support and equipment, drugs, or supplies were provided by Bio Farma Persero PT. Yetty Movieta Nency reports financial support and equipment, drugs, or supplies were provided by Bio Farma Persero PT. Martira Maddeppungeng reports financial support and equipment, drugs, or supplies were provided by Bio Farma Persero PT. Rini Sekartini reports financial support and equipment, drugs, or supplies were provided by Bio Farma Persero PT. Rini Mulia Sari reports a relationship with Bio Farma Persero PT that includes: employment. Fikrianti Surachman reports a relationship with Bio Farma Persero PT that includes: employment. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier India Pvt Ltd. All rights reserved.)

Mackerel fish (Scomberomorus spp.) represents a significant marine fisheries commodity in Indonesia, characterized by its high commercial value and nutritional content. To understand the intraspecific interactions and genetic variability of Scomberomorus spp., a more extensive research of Scomberomorus spp. populations, including both cultivated and wild specimens, is required. This study aimed to explore the genetic diversity of mackerel fish in Indonesian waters, focusing on the mitochondrial DNA (mtDNA) cytochrome oxidase subunit II (COII) gene, which encodes the second subunit of cytochrome c oxidase (complex IV), is essential for aerobic respiration and energy transformation. Muscle tissue samples from 18 individual mackerel fish collected from various regions in Indonesia, including Palembang, Cilacap, Rembang, Banjarmasin, Ambon, and Fak-Fak Regencies, were utilized. The genomic DNA was isolated and amplified using specific primers: CO2TF (5'-ACCGCTCTGTCACTTTCTTC-3') and CO2TR (5'-ATGTCACTAAGGGTGGTTGG-3'). Subsequently, the obtained amplicons were subjected to sequencing. The sequence data were then analyzed using the MEGA11 and DnaSP 6 software. Our findings revealed 120 variable sites within the 691 base pairs of mtDNA COII sequences, resulting in a nucleotide diversity (Pi) of 0.07169. Furthermore, we identified eight haplotypes, demonstrating a haplotype diversity (Hd) of 0.8889. Remarkably, all mackerel samples from Palembang and Cilacap clustered into discrete haplotypes, specifically haplotype 1 and haplotype 2, respectively. Our phylogenetic analysis delineated three distinct clades. Clade I, closely related to Scomberomorus cavalla, encompassed all individuals from Ambon, Palembang, Rembang, and one from Banjarmasin. Clade II, associated with Scomberomorus niphonius, included individuals from Cilacap and two from Banjarmasin. Clade III, linked to Scomberomorus semifasciatus, exclusively consisted of individuals from Fak-Fak (Papua). In conclusion, Indonesian waters harbor diverse genetic variations within Scomberomorus spp., and population relationships based on the mtDNA COII gene exhibit notable complexities. Future research endeavors should focus on further elucidating the diversity and relationships among Scomberomorus spp. in diverse Indonesian populations.

In this study, we aimed to evaluate the immunological protectivity of infants following four doses of bivalent oral polio vaccine (bOPV; Bio Farma), which were given simultaneously with DTwP-Hb-Hib (Pentabio®), along with one dose of inactivated poliovirus vaccine (IPV) at the fourth visit. A total of 143 newborn infants who fulfilled the inclusion criteria were enrolled and completed the study. Subjects received the first dose of bOPV at birth. On days 60, 90 and 120, bOPV was given simultaneously with Pentabio®. On day 120, one dose of IPV was also administered. Serum samples for serology analysis were collected before the first dose of bOPV (at day 0), before the second dose of bOPV (at day 60) and 30 days after the last dose of bOPV. In addition, the intensity, duration and relationship of each adverse event to the trial vaccines were assessed. Seroprotection rates after the fourth dose of bOPV were 100%, 91.6% and 99.3% for poliovirus P1, P2 and P3, respectively. Seroconversion rates after the fourth dose of bOPV were 100.0%, 93.3% and 100% for poliovirus P1, P2 and P3, respectively. There were no severe adverse events, and systemic reactions were generally mild during the 1-28 day post-vaccination period. Collectively, our findings indicate that bOPV given simultaneously with Pentabio® and one dose of IPV at the 4th visit was immunogenic and well tolerated., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.)