1. Dengue hemorrhagic fever in Jakarta, Indonesia in 1988: isolation of dengue virus from patient whole blood using cell cultures.
- Author
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Fujita N, Hotta S, Konishi E, Esaki H, Sumarmo, and Sujudi
- Subjects
- Aedes cytology, Animals, Cell Line, Child, Child, Preschool, Cricetinae, Dengue virology, Dengue Virus classification, Dengue Virus growth & development, Female, Hemagglutination Inhibition Tests, Humans, Indonesia epidemiology, Male, Viral Plaque Assay, Viremia virology, Dengue epidemiology, Dengue Virus isolation & purification, Disease Outbreaks, Viremia epidemiology
- Abstract
During an outbreak of dengue hemorrhagic fever (DHF) in Jakarta, Indonesia in 1988, we attempted to isolate dengue virus using mosquito cells and a medium containing heparin. Whole blood, immediately after being drawn from patients, was inoculated into Aedes albopictus cell cultures temporarily maintained in the heparin-containing medium. The overall virus isolation rate was 25% (17 of 69) samples collected within three days after admission of the patients to hospital. No virus was obtained thereafter. The successful virus isolation was apparently not related to titers of anti-dengue virus hemagglutination-inhibiting antibodies present in patients' sera. The viruses were recovered from cases of each of the four World Health Organization grades of DHF without significant differences. The technique is simple and easily performed at bedside.
- Published
- 1997
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