1. The antidepressant effect of Cynanchum auriculatum in mice.
- Author
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Ji CX, Li XY, Jia SB, Liu LL, Ge YC, Yang QX, and Zhang JJ
- Subjects
- Animals, Antidepressive Agents adverse effects, Antidepressive Agents isolation & purification, Antidepressive Agents pharmacology, Behavior, Animal drug effects, China, Dose-Response Relationship, Drug, Ethnopharmacology, Glycosides adverse effects, Glycosides isolation & purification, Glycosides pharmacology, Glycosides therapeutic use, India, Male, Mice, Mice, Inbred ICR, Phytotherapy, Plant Extracts adverse effects, Plant Extracts isolation & purification, Plant Extracts pharmacology, Rats, Rats, Sprague-Dawley, Serotonin metabolism, Selective Serotonin Reuptake Inhibitors adverse effects, Selective Serotonin Reuptake Inhibitors isolation & purification, Selective Serotonin Reuptake Inhibitors pharmacology, Synaptosomes drug effects, Synaptosomes metabolism, Antidepressive Agents therapeutic use, Cynanchum chemistry, Depression drug therapy, Drug Discovery, Plant Extracts therapeutic use, Rhizome chemistry, Selective Serotonin Reuptake Inhibitors therapeutic use
- Abstract
Context: Antidepressant effects of various plants are generally attributed to their anti-inflammation and antioxidant activities. Cynanchum auriculatum Royle ex Wight (Asclepiadaceae) is a traditional medicinal plant in China and India used for immunological regulation, anti-inflammation, and antioxidant purposes. However knowledge about its antidepressant activity has been poorly investigated., Objective: To investigate the antidepressant activities of the total glycosides of C. auriculatum (TGC) and its CHCl₃/MeOH (10:1) fractions (TGC-D and TGC-E) in mice., Materials and Methods: TGC, TGC-D and TGC-E (20, 40 and 80 mg/kg) were intragastrically administered to mice twice a day for 5 days. The tail suspension test, forced swimming test, and locomotor activity test in mice were used to evaluate the effect of C. auriculatum. The inhibition of [³H]-serotonin reuptake in rat brain synaptosomes was detected to investigate their mechanism., Results: TGC, TGC-D and TGC-E (80 mg/kg) decreased the immobility time by 61.7, 64.5, and 61.9% in tail suspension test. TGC (80 mg/kg), TGC-D (80 mg/kg) and TGC-E (20 mg/kg) decreased the immobility time by 32.6, 47.3, and 48.7% in forced swimming test. TGC (80 mg/kg) and TGC-E (20 and 40 mg/kg) decreased the crossing distances by 28.8, 29.5, and 36.2% in locomotor activity test. TGC, TGC-D and TGC-E (10 mg/L) inhibited serotonin reuptake by 7.4, 4.5, and 71.1% in rat brain synaptosomes, and IC₅₀ value of TGC-E was 5.2 mg/L., Discussion and Conclusion: TGC, TGC-D and TGC-E have potential antidepressant activities. The antidepressive effect of TGC-E maybe attributed partly by the inhibiting effect on serotonin reuptake.
- Published
- 2012
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