Search

In this study, we investigated the association of GSTP1, GSTM1, and GSTP1 genetic variants with renal cell carcinoma (RCC) among North Indian patients. The difference in frequency of the GSTT1 null genotype between cases and control subjects was statistically significant (active ver. null, odds ratio [OR]=0.368; confidence intervals [CI] 95%=0.243-0.557, p=0.001). The differences in the frequency of GSTP1 genotypes were statistically significant (AA ver. AG/GG, OR=1.879; CI 95%=0.355-0.797, p=0.002). Higher allelic frequency of the GSTP1 G allele was associated with RCC cases (G ver. A allele, OR=1.534; 95% CI=1.159-2.030, p=0.003). The gene-gene interaction in terms of three-way combination of GSTM1 null, GSTT1 null, and GSTP1 (AG/GG) resulted in 4.5-fold increase in RCC risk (OR=4.452; 95% CI=2.220-9.294). Similarly, our study revealed that GST polymorphism might be a vital determinant of advancement to higher pathological stages and histological grades of RCC. Our findings suggest that genetic variability in members of the GST gene family may be associated with an increased susceptibility to RCC and its progression.

Aberrant promoter hypermethylation of cancer associated genes occur frequently during carcinogenesis and may serve as a cancer biomarker. The aim of this study was to investigate the occurrence and relevance of promoter methylation of the tumor suppressor DAPK-1, APAF-1 () and SPARC in relation to different pathological stages and histological grades of tumor progression that might act as possible independent prognostic factor in the susceptibility towards renal cell carcinoma (RCC) in North Indian population. Three tumor suppressor gene promoters namely APAF-1, DAPK-1 and SPARC were assessed by methylation-specific PCR (MS-PCR) in 196 primarily resected renal cell tumors paired with the corresponding normal tissue samples. After genomic DNA isolation and sodium bisulfite modification, methylation levels were determined and correlated with standard clinicopathological parameters, pathological stage and Fuhrman nuclear grade of RCC. Significant differences in methylation frequency among the four subtypes of renal tumors were found for APAF-1 (p < 0.001), DAPK-1 (p < 0.001) and SPARC (p = 0.182), when compared with the corresponding normal tissue. Male subjects showed stronger association of methylation frequency of all the three genes with RCC than the female subjects. Additionally, higher frequency of APAF-1, DAPK-1 and SPARC promoter methylation were directly correlated with higher tumor stage (p (trend) < 0.001). Higher frequency of promoter methylation of APAF-1 and SPARC were also associated with higher nuclear grade (p < 0.001 and p = 0.036, respectively). This gene panel might contribute to a more optimal diagnostic coverage and information, improving preoperative assessment and therapeutic decision-making in patients harboring suspicious renal masses.

Background: Epidemiological studies are helpful in planning and implementing oral health programs in a given population. This initiative is a consequence of the absence of any information on any study being conducted in the past on the prevalence of dental diseases in the Barabanki district of Uttar Pradesh (UP)., Aims: The aims were to (1) assess the prevalence of gingivitis, fluorosis, and malocclusion in the school-going children of rural areas of district Barabanki and (2) evaluate the pattern of above-mentioned diseases in different age groups and genders., Materials and Methods: A total of 836 school-going children comprising 430 boys and 406 girls were examined. A total of 238 children were in the age group of 5-7 years. A total of 277 and 321 children were in the age groups of 8-10 and 11-14 years, respectively., Statistical Analysis: A chi-square test was used for deriving results., Results: The prevalence of gingivitis, fluorosis, and malocclusion was 78.35%, 33.37%, and 34.09%, respectively. The difference between age groups for the prevalence of gingivitis was highly significant, and was more among girls as compared to boys. A significant increase in the prevalence of malocclusion was observed with age. No significant difference was found among genders for the prevalence of malocclusion. An increase in the prevalence of fluorosis was highly significant with age. The difference in fluorosis was insignificant between boys and girls.