1. Rothia santali sp. nov., endophytic bacteria isolated from sandalwood (Santalum album L.) seedling.
- Author
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Tuikhar N, Kirdat K, Nair G, Padma S, Thorat V, Swetha P, Sathe S, Sundararaj R, and Yadav A
- Subjects
- Bacteria, India, Micrococcaceae, RNA, Ribosomal, 16S genetics, Seedlings, Oils, Volatile, Santalum genetics, Sesquiterpenes
- Abstract
A novel, mustard yellow-pigmented aerobic bacterial strain designated AR01
T was isolated from hypocotyl tissue of a sandalwood seedling from Bangalore, India. The 16S rRNA gene of strain AR01T had the highest 98.97% sequence similarity with Rothia halotolerans YIM 90716T (KCTC 19172) followed by Rothia kristinae PM 129T (NBRC 15354T ) (97.31%) and Rothia koreensis P31T (JCM 15915) (97.11%), respectively. The strain AR01T was coccoid-shaped, non-motile, non-spore forming, oxidase negative and catalase positive. The strain AR01T has a genome size of 3.31 Mb containing 2993 protein-coding genes including 48 tRNA and 10 rRNAs spread across 84 contigs. The genomic DNA G + C content was 71.77 mol%. The calculated dDDH was 31.10% and the OrthoANI value was 85.27% when compared with its closest related type strain Rothia halotolerans YIM 90716T . The predominant cellular fatty acids were C16:0 iso , C15:0 anteiso and C17:0 anteiso . The strain AR01T contains major polar lipids including diphosphatidylglycerol and phosphatidylglycerol. The distinct physiological, biochemical characteristics and genotypic relatedness indicated that AR01T represents a novel species of the genus Rothia, for which the name Rothia santali sp. nov. (Type strain AR01T = MCC 4800T = JCM 35593T ) is proposed., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2022
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