1. Lysobacter defluvii sp. nov., isolated from municipal solid waste.
- Author
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Yassin AF, Chen WM, Hupfer H, Siering C, Kroppenstedt RM, Arun AB, Lai WA, Shen FT, Rekha PD, and Young CC
- Subjects
- Bacterial Typing Techniques, Carbon metabolism, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Enzymes biosynthesis, Fatty Acids analysis, Genes, rRNA, India, Lysobacter chemistry, Lysobacter physiology, Molecular Sequence Data, Nitrogen metabolism, Phylogeny, Quinones analysis, RNA, Bacterial genetics, RNA, Ribosomal, 16S genetics, Refuse Disposal, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Lysobacter classification, Lysobacter isolation & purification, Soil Microbiology
- Abstract
A bacterial isolate obtained from soil from a municipal landfill site in India was characterized using a polyphasic taxonomic approach. The colonies of the isolate were found to be yellow and highly mucoid. Comparative analysis of the 16S rRNA gene sequence showed that this isolate constitutes a distinct phyletic line within the genus Lysobacter, displaying >3 % sequence divergence with respect to recognized Lysobacter species. The generic assignment was confirmed by chemotaxonomic data, which revealed the presence of a fatty acid profile characteristic of members of the genus Lysobacter and consisting of saturated, unsaturated, straight-chain and branched-chain fatty acids as well as iso-C(11 : 0) 3-OH as hydroxylated fatty acid, and the presence of an ubiquinone with eight isoprene units (Q-8) as the predominant respiratory quinone. The genotypic and phenotypic data show that strain IMMIB APB-9(T) merits classification as representing a novel species of the genus Lysobacter, for which the name Lysobacter defluvii sp. nov. is proposed. The type strain is IMMIB APB-9(T) (=CCUG 53152(T)=DSM 18482(T)).
- Published
- 2007
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