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Holy basil (Ocimum sanctum L.) and sweet basil (Ocimum basilicum L.) are the most commonly grown basil species in India for essential oil production and biosynthesis of potentially volatile and non-volatile phytomolecules with commercial significance. The aroma, flavor and pharmaceutical value of Ocimum species is a significance of its essential oil, which contains most of the monoterpenes and sesquiterpenes. A large number of plants have been studied for characterization and identification of terpene synthase genes, involved in terpenoids biosynthesis. The goal of this study is to discover and identify the putative functional terpene synthase genes in O. sanctum. HMMER search was performed by using a set of 13 well sequenced and annotated plant genomes including the newly sequenced genome of O. sanctum with Pfam-A database locally, using HMMER 3.0 hmmsearch for the two Pfam domains (PF01397 and PF03936). Using this search method 81 putative terpene synthases genes (OsaTPS) were identified in O. sanctum; the study further reveals 47 OsaTPS were putatively functional genes, 19 partial OsaTPS, and 15 OsaTPS as probably pseudogenes. All these identified OsaTPS genes were compared with other plant species, and phylogenetic analysis reveals the subfamily classification of OsaTPS in TPS-a, -b, -c, -e, -f and TPS-g subfamilies clusters. This genome-wide identification of OsaTPS genes, their phylogenetic analysis and secondary metabolite pathway mapping predictions together provide a comprehensive understanding of the TPS gene family in Ocimum sanctum and offer opportunities for the characterization and functional validation of numbers of terpene synthase genes. [ABSTRACT FROM AUTHOR]

Background: Ocimum sanctum L. (O. tenuiflorum) family-Lamiaceae is an important component of Indian tradition of medicine as well as culture around the world, and hence is known as "Holy basil" in India. This plant is mentioned in the ancient texts of Ayurveda as an "elixir of life" (life saving) herb and worshipped for over 3000 years due to its healing properties. Although used in various ailments, validation of molecules for differential activities is yet to be fully analyzed, as about 80% of the patents on this plant are on extracts or the plant parts, and mainly focussed on essential oil components. With a view to understand the full metabolic potential of this plant whole nuclear and chloroplast genomes were sequenced for the first time combining the sequence data from 4 libraries and three NGS platforms. Results: The saturated draft assembly of the genome was about 386 Mb, along with the plastid genome of 142,245 bp, turning out to be the smallest in Lamiaceae. In addition to SSR markers, 136 proteins were identified as homologous to five important plant genomes. Pathway analysis indicated an abundance of phenylpropanoids in O. sanctum. Phylogenetic analysis for chloroplast proteome placed Salvia miltiorrhiza as the nearest neighbor. Comparison of the chemical compounds and genes availability in O. sanctum and S. miltiorrhiza indicated the potential for the discovery of new active molecules. Conclusion: The genome sequence and annotation of O. sanctum provides new insights into the function of genes and the medicinal nature of the metabolites synthesized in this plant. This information is highly beneficial for mining biosynthetic pathways for important metabolites in related species. [ABSTRACT FROM AUTHOR]

Bayana Basin, sited along the eastern margin of the north Delhi fold belt of the Aravalli Craton, contains an ∼3000 m-thick sequence comprising one volcanic and seven sedimentary formations of the Delhi Supergroup. The sedimentary units are the Nithar, Jogipura, Badalgarh, Bayana, Damdama, Kushalgarh, and Weir formations in order of decreasing age. Petrographic study of the sandstones as well as major and trace elements (including rare earth elements) and bulk-rock analyses of the shales and sandstones allow the determination of their provenance, source-rock weathering, and basinal tectonic setting. The sandstones are quartz rich and were derived mainly from exhumed granitoids typical of a craton interior. Geochemical patterns of the sandstones and shales are similar. However, trace element abundances are low in sandstones, probably due to quartz dilution. The coarser clastic Damdama and Weir sandstones, which occur at higher stratigraphic levels, have strikingly low trace element concentrations compared with the underlying Bayana and Badalgarh sandstones. All samples show uniform LREE-enriched patterns with negative Eu-anomalies (Eu/Eu* = 0.16–0.23) and are similar to those of post-Archaean Australian shales (PAAS). However, the (La/Yb) n ratios (averages 11–18) of all the sedimentary units are higher than those of PAAS, except for the Bayana Sandstone, which has low values (average 6.77). The chemical index of alteration (70–78) and the plagioclase index of alteration (87–97) values and the A–CN–K diagram suggest moderate to intense weathering of the source area. The provenance analyses indicate that basin sedimentation was discontinuous. It received input from a terrain comprising granitoids, mafic rocks, sedimentary sequences, and tonalite-trondhjemite-granodiorite (TTG) suites. The Nithar and Badalgarh sandstones received input from a source consisting predominantly of granitoids. The succeeding Damdama and Weir sandstones received debris from granitoids and TTG in different proportions. The Kushalgarh shale was possibly derived from a source consisting granites and mafic rocks with a TTG component. The pre-existing sedimentary formations also contributed intermittently during the different phases of sedimentation. Bulk-rock geochemical data suggest Mesoarchaean gneisses and late Archaean granites of BGC/BGGC (Banded Gneissic Complex/Bundelkhand Granitic Gneiss Complex) basement as possible source terrains. These data indicate deposition in a continental rift setting. The coeval formation of many rift-related Proterozoic sedimentary basins in the BGC/BGGC terrain suggests that the North Indian Craton underwent major intracratonic extension during Proterozoic time, probably triggering the break up of Earth's first supercontinent. [ABSTRACT FROM AUTHOR]

For Pakistan, nuclear capability is an instrument of war prevention and insurance against invasion. Its nuclear doctrine is heavily influenced by India's conventional superiority, making it difficult for Pakistan to eschew first use and setting its deterrence policy apart from India's. [ABSTRACT FROM PUBLISHER]

The article examines past arms control agreements and confidence-building measures undertaken between India and Pakistan, and how they could be made more effective in the future. Major agreements and treaties between the two countries since partition in 1947 are reviewed, including the 1949 Karachi Agreement following the Kashmir War, the 1966 Tashkent Agreement following the 1965 Indo-Pakistani conflict, the 1999 Lahore Agreement over nuclear weapons, and others. The strategic concerns and military doctrine of each nation are discussed as well.

Saraca asoca bark has been used in the Ayurvedic system of medicine for female urino-genital disorders. We have recently reported the isolation and characterization of several compounds as markers to develop HPLC profiling of its methanol and aqueous methanol extracts. Now, a HPLC-PDA inactive compound, (+)-pinitol has been isolated and characterized from the bark of this medicinally important tree. Pinitol is a well known bioactive compound for a variety of biological activities, including hypoglycemic and anti-inflammatory activities. A process for the isolation of relatively good concentration of (+)-pinitol from S. asoca bark has been developed and its in vitro anti TNF-α and anti-inflammatory activities against carragenan-induced edema confirmed. While conducting experiments on the possible agonistic activity, it was found that (+)-pinitol showed up to eight fold reduction in the doses of β-lactam antibiotics. The mechanism of its agonistic activity was studied by docking experiments which showed that different conformations of (+)-pinitol and antibiotics were actually in the same binding site with no significant change in the binding energy. These docking simulations, thus predict the possible binding mode of studied compounds and probable reason behind the synergistic effect of (+)-pinitol along with β-lactam antibiotics.

Immunomodulation is the process of alteration in immune response due to foreign intrusion of molecules inside the body. Along with the available drugs, a large number of herbal drugs are promoted in traditional Indian treatments, for their immunomodulating activity. Natural coumarinolignoids isolated from the seeds of Cleome viscose have been recognized as having hepatoprotective action and have recently been tested preclinically for their immunomodulatory activity affecting both cell-mediated and humoral immune response. To explore the immunomodulatory compound from derivatives of coumarinolignoids, a quantitative structure activity relationship (QSAR) and molecular docking studies were performed. Theoretical results are in accord with the in vivo experimental data studied on Swiss albino mice. Immunostimulatory activity was predicted through QSAR model, developed by forward feed multiple linear regression method with leave-one-out approach. Relationship correlating measure of QSAR model was 99% (R(2) = 0.99) and predictive accuracy was 96% (RCV(2) = 0.96). QSAR studies indicate that dipole moment, steric energy, amide group count, lambda max (UV-visible), and molar refractivity correlates well with biological activity, while decrease in dipole moment, steric energy, and molar refractivity has negative correlation. Docking studies also showed strong binding affinity to immunomodulatory receptors.

Background: The relevance of population-based cancer registries for planning and implementing cancer control programmes cannot be overemphasized. There are some urban registries in India but very few rural registries despite India being predominantly rural. There are several obstacles to setting up a rural registry including lack of cancer awareness in the rural population and inaccessibility of modern medical facilities. The first rural cancer registry was set up in 1987 at Barshi (population 0.4 million) in western Maharashtra by adopting a methodology suitable for rural areas., Methods: The innovative methodology supplemented the usual registry methodology by regular interaction with the community to educate them on warning signals for cancer, raise cancer awareness and motivate suspected individuals to seek medical attention. Cancer detection clinics were held in villages., Results: The reliability indices show that the registry is of an acceptable standard. The registry activity has increased cancer awareness in this population (p < 0.01), increased the frequency of early cervical cancers (stages I and IIa) by more than 2-fold during the past 16 years and significantly decreased the relative risk of death (hazard ratio 0.7 [0.5-0.9])., Conclusion: The innovative methodology has facilitated the process of cancer registration in rural areas. It has had a positive impact on cancer awareness, stage at presentation and survival of cervical cancers-the predominant cancer in the area. The registry has created a resource for epidemiological studies in a rural area where national and international studies are currently being undertaken.