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To know prevalence of rotavirus diarrhea subgroups and serotypes, a prospective study was conducted in rural, periurban and urban communities at Chandigarh. Weekly surveillance for diarrheal episodes was carried out in 110 families each from rural, periurban and urban localities constituting 584 children < 5 years of age from October, 1988 to February, 1991. Stool samples of 218 diarrheal episodes occurring in 115 children were subjected to rotavirus detection by ELISA. Rotavirus positive samples were further analyzed for subgroups and serotypes using specific monoclonal antibodies. Overall prevalence of rotavirus diarrhea was 4.3% (25/584). Rotavirus constituted 11.5% (25/218) of total diarrheal episodes and 22% (25/115) among the children affected with acute diarrhea. Among rural, periurban and urban communities, the overall prevalences of rotavirus diarrhea were 7.3%, 3.2% and 2.3% and episode related prevalences of 31.8%, 7.4% and 5%, respectively (chi 2 test for trend was highly significant from rural to periurban to urban localities). Forty per cent (10/25 of rotavirus positive samples were subgroup I and 60% (15/25) sub-group II. Of the 25 rotavirus strains, 40% (10) were serotype 2, 24% (n = 6) serotype 3 and 36% (n = 9) serotype 4. No definite temporal or seasonal pattern of rotavirus was observed; however, more of rotavirus diarrheal episodes (16%) occurred during winter season. Subgroups and serotypes were observed to cocirculate during the rotavirus episodes. Demonstration of serotypes in our field study imply that the vaccine to be used in our country must be cross protective to have an effective impact on rotavirus infection.(ABSTRACT TRUNCATED AT 250 WORDS)

Preschool children of two villages of Kharar Taluka, Ropar district, Punjab (India) were screened for the prevalence of giardiasis. Cysts of Giardia lamblia were found in 35.1% of the stool samples and other parasites were rarely seen. The incidence of diarrhoea in association with G. lamblia positivity was 16.5% in subjects whose stool examination was positive on one or more than one occasion. No difference in the incidence of giardiasis could be seen in well nourished and undernourished children living in these endemic areas.