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Contents Seasonal behaviour in sheep, which varies in tropical and temperate environmental conditions, is a matter of study, because it can provide a clue to address the problem of seasonality in sheep. Melatonin receptor is the membrane-bound G-coupled receptor, sensing the message of photoperiodic cues thorough melatonin. Restriction fragment length polymorphism ( RFLP) studies were carried out to assess the variability of gene at G612A and C606T SNPs in MTNR1A gene, which have been studied to be markers for out-of-season breeding. Allelic frequency distribution corresponded to higher frequency of GG and CC genotype, in tropical arid sheep breed in comparison with temperate region sheep breed. PCR amplification of MTNR1A gene of 30 animals was performed and single nucleotide polymorphisms ( SNPs) identification was carried out using Lasergene software. Seven SNPs/mutations were identified, but most of them were synonymous, except the one G706A, leading to substitution of valine by isoleucine. Polyphen-2 analysis of G706A mutation revealed that it is a benign mutation. Two important SNPs C426T and G555A, which were identified in temperate sheep breeds, could not be traced in Magra and Marwari breeds of sheep. Thus, the Magra and Marwari breeds of tropical, arid region demonstrated the presence of both polymorphic SNPs markers G612A and C606T, associated with out-of-season breeding. GG and CC genotypes were having a higher prevalence in the studied population. [ABSTRACT FROM AUTHOR]

In this paper, we construct the SV1V2EIR model to reveal the impact of two-dose vaccination on COVID-19 by using Caputo fractional derivative. The feasibility region of the proposed model and equilibrium points is derived. The basic reproduction number of the model is derived by using the next-generation matrix method. The local and global stability analysis is performed for both the disease-free and endemic equilibrium states. The present model is validated using real data reported for COVID-19 cumulative cases for the Republic of India from 1 January 2022 to 30 April 2022. Next, we conduct the sensitivity analysis to examine the effects of model parameters that affect the basic reproduction number. The Laplace Adomian decomposition method (LADM) is implemented to obtain an approximate solution. Finally, the graphical results are presented to examine the impact of the first dose of vaccine, the second dose of vaccine, disease transmission rate, and Caputo fractional derivatives to support our theoretical results.

The present study was conducted to detect aerobic causative agents of urinary tract infection (UTI) and their antibiogram pattern. This study was carried out in the Department of Microbiology, Regional Institute of Medical Sciences (RIMS), Imphal, Manipur, India. A total of 1,109 clean catched midstream urine samples were collected, out of which 459 (40.4%) samples grew potential pathogens causing UTI. Escherichia coli were the predominant 334 (72.8%) bacterial pathogen followed by Klebsiella species 66 (14.4%), non lactose fermenters 19 (4.1%), Pseudomonas species 16 (3.5%) and others. Most of the strains of E. coli were resistance to Ciprofloxacin whereas sensitive to Aminoglycoside. Most of the urinary isolates showed high degree of resistance to Tetracycline, Norfloxacin and Cotrimoxazole. Gentamycin was the drug of choice for most of the strains.