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During recent survey for the investigation of foliar fungi in Kerala, India, a new species of foliicolous hyphomycete, Paramyrothecium kamalii was discovered on living leaves of Matourea azurea (Plantaginaceae) based on morpho-cultural characteristics and multigene (ITS, LSU, cmdA, tub2, and rpb2) phylogenetic analysis; is described, illustrated and discussed. In vitro Pathogenicity tests were performed and confirmed the pathogenic nature of the fungus, thereby fulfilling Koch's postulates. Phylogenetically, P. kamalii forms a distinct clade, closely related to P. verroridum, P. indicum, and P. roridum. However, it differs morphologically by having longer conidiophores and conidia and lacks setae, unlike the closer species. The identification of P. kamalii as a new foliar pathogen provides critical insights for surveillance and effective control measure of this foliar disease of M. azurea in tropical ecosystems., Competing Interests: Declarations. Disclosure statement: The authors declare that they have no conflict of interest to develop this research work. Competing interests: The authors declare no competing interests., (© 2025. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Cotton leaf curl disease (CLCuD) is a major constraint for production of cotton (Gossypium sp.) in Northwest India. CLCuD is caused by a monopartite, circular ssDNA virus belonging to the genus Begomovirus in association with betasatellites and alphasatellites, and ttransmitted by a whitefly vector (Bemisia tabaci). To explore the genetic variability in betasatellites and alphasatellite associated with the CLCuD-begomovirus complex in Northwest India. A survey was conducted for successive three years of 2014 to 2016 and twig samples from symptomatic and healthy cotton plants randomly were collected. Total plant DNAs were isolated, subjected to rolling circle amplification (RCA), cloning and sequencing. Full-length genome of 12 betasatellites and 13 alphasatellites, those were obtained in the present study, were analyzed. Sequence analysis showed that all the present betasatellites shared 85-99 percent nucleotide identity (PNI) among themselves and 84-95 PNI with other members of Cotton leaf curl Multan betasatellite (CLCuMB) and fell into one genogroup along with CLCuMB. But in close observation the present betasatellites clustered into two phylogenetic subgroups under single CLCuMB. The present alphasatellites showed 72-100 PNI among themselves and fell under three alphasatellite species, Gossypium Darwinii symptomless alphasatellite (GDarSLA), Cotton leaf curl Multan alphasatellite (CLCuMA) and Cotton leaf curl Burewala alphasatellite (CLCuBuA). In the recombination analysis, all the present betasatellites and alphasatellites were found to be recombinants involving intra species recombination in betasatellite, and interspecies recombination in alphasatellite species. The present study indicated that the betasatellite and alphasatellite molecules associated with CLCuD-begomovirus complex in Northwest India are genetically diverse., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2025 Biswas et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

An in-depth understanding of carbon dynamics and ecosystem productivity is essential for conservation and management of different ecosystems. Ecosystem dynamics and carbon budget are assessed by estimating net ecosystem production (NEP) across different global ecosystems. An ecological productivity assessment of forest and floating meadow ecosystems in Keibul Lamjao National Park (KLNP), Manipur, North East India, was conducted using the multi-criteria decision-making process namely, gray relational analysis (GRA). The analysis was performed on 24 selected criterions classified either as "higher-the-better" or "lower-the-better" based on their degree of influence on the carbon budget. Floating meadows exhibited a higher production of aboveground and belowground biomass and a higher total mortality and decay. Furthermore, the study found that floating meadows exhibited a higher soil organic carbon (SOC) and net soil organic matter (SOM) than the forest ecosystem. The forest ecosystem showed higher total respiration (R T ), heterotrophic respiration (R H ), and autotrophic respiration (R A ) than floating meadows. Floating meadows exhibited a higher net primary productivity (NPP) of 616.49 ± 33.87 gCm -2  year -1 than the forest ecosystem, which has a NPP of 566.64 ± 65.26 gCm -2  year -1 . Similarly, floating meadows have higher NEP (495.25 ± 36.46 gCm -2  year -1 ) than forest ecosystems (418.39 ± 65.76 gCm -2  year -1 ). These characteristics have a significant influence on the carbon budget in floating meadows as compared to forest ecosystems, as shown by larger values of gray relational coefficient (GRC) in GRA. The floating meadows ecosystem (0.82) obtained 54.72% gain in gray relational grades (GRG) value with the forest ecosystem (0.53). This study might help in improving KLNP and other adjutant areas for conservation and management policies from the vital information given on the importance of wetlands in carbon dynamics and ecosystem productivity., Competing Interests: Declarations. Ethical approval: The authors confirm that all studies described in this manuscript were carried out in an ethical and responsible manner, adhering fully to all pertinent codes of experimentation and legislation. Competing interests: The authors declare no competing interests., (© 2025. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Mangroves are key components of productive ecosystems that provide a multitude of ecosystem goods and services. How these species will respond to future climates with more frequent and severe extreme temperatures has not received much attention. To understand how vulnerable mangroves are to future warming, we quantified photosynthetic heat tolerance and estimated thermal safety margins for thirteen mangrove species from the southwestern Indian coast. We quantified heat tolerance as temperatures that resulted in a 5 % (T 5 ) and 50 % (T 50 ) decline in photosystem II function, and thermal safety margins (TSM) as the difference between T 50 and maximum leaf temperatures. T 50 ranged from 48.9 °C in Avicennia Marina to 55.3 °C in Bruguiera gymnorhiza, with a mean of 53.3 °C for the thirteen species. Heat tolerance was higher for species with bigger leaves which experience higher leaf temperatures, but was not related to the other leaf traits examined. Heat tolerance was exceptionally high in these mangroves compared to other woody species. With their high tolerance and large safety margins these mangroves may be relatively less vulnerable to future climates with higher temperatures., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Wetlands are important ecosystems, and few of them, owing to unique characteristics, have been designated the status of Ramsar sites under the convention of wetlands. Despite the status, few of these wetlands are under threat owing to their social importance. Renuka wetland is one such important Ramsar site in India, which is culturally an embodiment of the Goddess Renuka ji. An international fair is held every year at the lake which attracts thousands of pilgrims and tourists. This event is one of the major contributors to anthropogenic risk; other pressures are siltation, pollution, land use activities, and invasive species. Under such a scenario, it becomes important to assess the health of wetlands to take timely decisions about their management. This study evaluates the health of Renuka wetland, using the Pressure State and Response (PSR) model. Relevant data related to the wetland and its catchment from a wide spectrum of sources, namely, field, laboratory, and remote sensing were combined to assess the health of the wetland. Ten indicators were identified, and Analytic Hierarchy Process (AHP) was used to assign weights to the indicators. The wetland health index of Renuka wetland is 0.35, indicating that the lake is in poor condition exhibiting degraded functioning, high external pressure, low vigor, poor resilience, and stability. The study also revealed that the water quality deteriorates post-fair. Thus, the wetland needs attention in terms of management so that the health of the lake improves and is sustained in the future. The study contributes to Sustainable Development Goal 6.6 which focuses on protecting and restoring water-related ecosystems and aims at healthier ecosystems., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Land degradation is accelerating in the Himalayan ecosystem, resulting in the loss of soil nutrients due to severe erosion. Soil erosion presents a significant environmental challenge, resulting in both on-site and off-site consequences, such as reduced soil productivity and siltation in reservoirs. Soil erodibility (K factor), an inherent soil property, determines the susceptibility of soils to erosion. Sampling across hilly and mountainous terrain pose challenges due to its complex landscape. Despite these challenges, it is essential to study K factor variations in different land use/land cover types to comprehend the threat of erosion. Digital soil mapping offers an opportunity to overcome this limitation by providing spatial predictions of soil properties. The objective of our study is to map the spatial distribution of soil erodibility using the Random Forest (RF) model, a machine learning method based on sampled in situ soil data and environmental covariates. We collected 556 surface soil samples from the mountainous catchment (Tehri dam catchment) using the stratified random sampling approach. The model performed satisfactorily in both training (r 2  = 0.91; RMSE = 0.00185) and testing (r 2  = 0.45; RMSE = 0.00318) phases. Subsequently, we generated a digital map with a resolution of 12.5 m to depict the distribution of the K factor. Our analysis revealed that key environmental variables influencing the prediction of the K factor included geology, mean NDVI, and climatic factors. The average K factor value was estimated at 0.0304 and ranging from 0.0251 to 0.0400 t ha h ha -1  MJ -1  mm -1 . A higher K factor was observed in the barren land (0.0344) primarily located in the higher and trans-Himalayan region of seasonally snow-covered areas. These areas typically feature young soils with weak soil formation and unstable soil aggregates. Subsequently cropland/cultivated soils (0.0307) exhibited higher K factor values due to the breakdown of soil aggregates by ploughing activities and exposing carbon to decomposition. The average K factor value of evergreen (0.0294) and deciduous (0.0295) forests were the lowest compared to other land use/land cover types indicating the role of forests in resisting soil erosion. By assessing and predicting soil erodibility, land planners and farmers can implement erosion control measures to protect soil health, prevent sedimentation in water bodies, and sustain agricultural productivity in the Himalayas., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

The study characterized the temporal and spatial variability in greenhouse gas (GHG) fluxes (CO 2 , CH 4 , and N 2 O) between December 2020 and November 2021 and their regulating drivers in the subtropical wetland of the Indian Himalayan foothill. Five distinct habitats (M1-sloppy surface at swamp forest, M2-plain surface at swamp forest, M3-swamp surface with small grasses, M4-marshy land with dense macrophytes, and M5-marshy land with sparse macrophytes) were studied. We conducted in situ measurements of GHG fluxes, microclimate (AT, ST, and SMC (v/v) ), and soil properties (pH, EC, N, P, K, and SOC) in triplicates in all the habitat types. Across the habitats, CO 2 , CH 4 , and N 2 O fluxes ranged from 125 to 536 mg m -2  h -1 , 0.32 to 28.4 mg m -2  h -1 , and 0.16 to 3.14 mg m -2  h -1 , respectively. The habitats (M3 and M5) exhibited higher GHG fluxes than the others. The CH 4 flux followed the summer > autumn > spring > winter hierarchy. However, CO 2 and N 2 O fluxes followed the summer > spring > autumn > winter. CO 2 fluxes were primarily governed by ST and SOC. However, CH 4 and N 2 O fluxes were mainly regulated by ST and SMC (v/v) across the habitats. In the case of N 2 O fluxes, soil P and EC also played a crucial role across the habitats. AT was a universal driver controlling all GHG fluxes across the habitats. The results emphasize that long-term GHG flux monitoring in sub-tropical Himalayan Wetlands has become imperative to accurately predict the near-future GHG fluxes and their changing nature with the ongoing climate change., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

This study investigates the diversity and composition of soil bacterial communities in the rhizosphere of Attapadi and Nelliyampathy, prominent hill stations in Palakkad district, Kerala, India. The persistent flooding and landslides in 2018 and 2019 significantly impacted agricultural productivity in these regions. Utilizing high-throughput 16S rRNA gene sequencing (Illumina MiSeq), we conducted a comprehensive analysis of soil samples. Correlative assessments between soil parameters and microbial relative abundance at the phylum level revealed noteworthy positive associations. Notably, nitrogen (N) exhibited a positive relation with Crenarchaeota, Chloroflexi, Actinobacteriota, and Acidobacteriota; pH correlated with Firmicutes; organic carbon (OC) with WPS-2; and phosphorous with Proteobacteria. A total of 31,402 operational taxonomic units (OTUs) were identified, with the highest feature counts observed in undisturbed soils from Attapadi (AUD) and Nelliyampathy (NUD) (13,007 and 12,915, respectively). Disturbed soils in Nelliyampathy (ND) and Attapadi (AD) displayed a substantial decline in microbial diversity and composition, harbouring 1409 and 4071 OTUs, respectively. Alpha and beta diversity indices further underscored the more severe impairment of ND soils compared to AD soils. Interestingly, a majority of ND samples were landslide-affected (four out of five), while flood-affected soils accounted for four out of six AD samples. This indicates that landslides exert a more pronounced impact on microbial diversity and composition than floods. The observed decline in microbial count, composition, and diversity, even after 2 years of the disaster, raises concerns about potential threats to agricultural output. The findings emphasize the need for corrective measures, including the incorporation of microbial inoculum, to restore soil fertility in post-disaster landscapes., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Spatial distribution and edaphic influences on soil organic carbon (SOC) are key determinants of carbon sequestration potential, and analysis of aggregate-protected SOC gives an in-depth understanding of the stability of carbon stored in soils. The present study evaluated the edaphic effects on the SOC in four different forest types-tropical evergreen forest, tropical moist deciduous forest, tropical dry deciduous forest and shola forest-in the southern high hills agro-ecological zone of Western Ghats, India. SOC stocks at depths of up to 1 m varied significantly across forest types, with the highest levels observed in the shola forest type (441.08 Mg C/ha) and the lowest in the dry deciduous forest (138.17 Mg C/ha). Around 70% of SOC was found in upper layers (0-30 cm) in all the studied forest types. Evaluation by a fixed-effect model showed that forest type, soil depth and aggregate size significantly affected SOC storage in these systems. An assessment of the relative importance and effect of 14 edaphic factors on SOC content in different forest types using the random forest model showed that the algorithm could explain 93.68%, 41.72%, 45.53% and 75.2% variability of SOC concentration across shola, dry deciduous, moist deciduous and evergreen systems, respectively. Across all forest types, except for dry deciduous forests, soil texture was found to be the primary factor influencing SOC, surpassing all other edaphic parameters. Ionic interactions by way of metal oxides like Ca 2+ , Al 3+ , Fe 3+ , Mg and H + influenced the SOC in tropical forest systems. The insights into SOC dynamics and the edaphic factors regulating them offer valuable guidance for forest management in tropical regions, particularly regarding climate change mitigation., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Eucalyptus spp. are undoubtedly one of the most favored plantation trees globally. Accurately identifying Eucalyptus pathogens is therefore crucial for timely disease prevention and control. Recently, symptoms of a leaf blight disease were observed on Eucalyptus trees in plantations at Jhajjar and Karnal in the state of Haryana, northern India. Asexual morphs resembling the features of the Botryosphaeriaceae were consistently isolated from the symptomatic leaves. Morphological features coupled with DNA sequence analysis confirmed a novel species, which is described and illustrated here as Botryosphaeria eucalypti sp. nov. Conidia of the new taxon are longer and wider than those of its phylogenetic neighbors. A distinct phylogenetic position for the new taxon was established through combined analysis of the internal transcribed spacer (ITS), partial translation elongation factor-1α (tef1) and partial β-tubulin (tub2) regions. Recombination analysis provided additional support for the new species hypothesis. The pathogenicity of the novel species was proved on Eucalyptus leaves, and Koch's postulates were fulfilled. The discovery of new Botryosphaeria species is important because it will help in understanding the species diversity, host range, possible threats and disease control in the long run., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Advertisement calls in frogs have evolved to be species-specific signals of recognition and are therefore considered an essential component of integrative taxonomic approaches to identify species and delineate their distribution range. The species rich genus Microhyla is a particularly challenging group for species identification, discovery and conservation management due to the small size, conserved morphology and wide distribution of its members, necessitating the need for a thorough description of their vocalization. In this study, we provide quantitative description of the vocal behaviour of Microhyla nilphamariensis , a widely distributed south Asian species, from Delhi, India, based on call recordings of 18 individuals and assessment of 21 call properties. Based on the properties measured acrossed 360 calls, we find that a typical advertisement call of M. nilphamariensis lasts for 393.5 ±  57.5 ms, has 17 pulses on average and produce pulses at rate of 39 pulses/s. The overall call dominant frequency was found to be 2.8 KHz and the call spectrum consisted of two dominant frequency peaks centered at 1.6 KHz and 3.6 KHz, ranging between 1.5-4.1 KHz. Apart from its typical advertisement call, our study also reveals the presence of three 'rare' call types, previously unreported in this species. We describe variability in call properties and discuss their relation to body size and temperature. We found that overall dominant frequency 1 (spectral property) was found to be correlated with body size, while first pulse period (temporal property) was found to be correlated with temperature. Further, we compare the vocal repertoire of M. nilphamariensis with that of the congener Microhyla ornata from the western coast of India and Sri Lanka and also compare the call properties of these two populations of M. ornata to investigate intra-specific call variation. We find statistically significant differentiation in their acoustic repertoire in both cases. Based on 18 call properties (out of 20), individuals of each locality clearly segregate on PCA factor plane forming separate groups. Discriminant function analysis (DFA) using PCA factors shows 100% classification success with individuals of each locality getting classified to a discrete group. This confirms significant acoustic differentiation between these species as well as between geographically distant conspecifics. The data generated in this study will be useful for comparative bioacoustic analysis of Microhyla species and can be utilized to monitor populations and devise conservation management plan for threatened species in this group., Competing Interests: The authors declare there are no competing interests., (©2024 Srigyan et al.)

Background: Teak (Tectona grandis L.f.), an important source of tropical timber with immense economic value, is a highly outcrossing forest tree species. 150 unrelated accessions of teak (Tectona grandis L.f.) plus trees assembled as clones at National Teak Germplasm Bank, Chandrapur, Maharashtra, India was investigated for association mapping of candidate lignin biosynthesis gene (CAD1) and transcription factors (MYB1 and MYB2)., Methods and Results: The CAD1, MYB1 and MYB2 were amplified using specifically designed primers. The amplified sequences were then sequenced and genotyped for 112 SNPs/11 indels. We evaluated the association between SNPs and wood density in teak accessions using GLM and MLM statistical models, with Bonferroni correction applied. The teak accessions recorded an average wood density of 416.69 kg.m -3 (CV 4.97%) and comprised of three loosely structured admixed sub-populations (K = 3), containing 72.05% genetic variation within sub-populations with low intragenic LD (0-21% SNP pairs) at P < 0.05 and high LD decay (33-934 bp) at R 2  = 0.1. GLM and MLM models discounting systematic biases (Q and K matrices) to avoid false discovery revealed five loci at rare variants (MAF 0.003) and three loci at common variants (MAF 0.05) to be significantly (P < 0.05) associated with the wood density. However, the stringent Bonferroni correction (4.06-7.04 × 10 -4 ) yielded only a single associated locus (B1485C/A) from exon of MYB1 transcription factor, contributing to about 10.35% phenotypic variation in wood density trait., Conclusion: Scored SNP locus (B1485C/A) can be developed as a molecular probe for selection of improved planting stock with proven wood density trait for a large-scale teak plantation., (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)

Potholes are microhabitats in riverine ecosystems that substantially contribute to the unique regional faunal diversity and community composition. Investigations on the ecological status of potholes in riverine habitats are very scanty. The present work is of utmost importance, particularly as a pioneering study to evaluate the health status of riverine potholes using aquatic insects as potential biological indicators. Samples of water and aquatic insects were collected and analyzed during the pre-monsoon season from February 2022 to May 2022 in the potholes at Athirappilly and the Ezhattumugham regions of the Chalakudy river basin. The present study identified 208 individuals represented by 15 families in the potholes at Athirappilly, whereas 94 individuals belong to 10 families at Ezhattumugham. The computation of water quality parameters and benthic metrics revealed a significant variation between the stations. Spearman correlation analysis revealed that the water temperature, electrical conductivity, dissolved oxygen concentration, and biochemical oxygen demand were influencing the distribution of aquatic insects in the potholes. Families such as Perlidae, Ephemeridae, Baetidae, Stenopsychidae, and Hydropsychidae (sensitive families) reported from the well-oxygenated potholes at Athirappilly, where the water temperature and biochemical oxygen demand were minimum. The significantly high percentage composition of Ephemeroptera, Plecoptera, and Trichoptera taxa and a low family biotic index value indicate good health condition of the potholes at Athirappilly. In contrast, the low percentage composition of Ephemeroptera, Plecoptera, and Trichoptera taxa and high family biotic index values, with the abundance of tolerant families (Micronectidae, Caenidae, and Chironomidae), reflect the impact of organic pollution at Ezhattumugham., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Populus deltoides is one of the most favored cash crops in northern India. Thus, accurate identification of pathogens affecting P. deltoides is a critical step in finding or developing effective control measures. In June 2020, symptoms of a leaf blight disease were observed on P. deltoides trees planted at Forest Research Institute, Dehradun, India. Calonectria-like fungal isolates were consistently isolated from the infected leaf samples. Morphological features coupled with phylogenetic analysis of combined partial actin (act), calmodulin (cmdA), histone (his3), translation elongation factor 1-alpha (tef1) and β-tubulin (tub2) gene regions of two fungal isolates confirmed a novel species, which is described and illustrated here as Calonectria populi sp. nov. Symptoms similar to those observed in natural conditions were caused by both the isolates on P. deltoides clone AM109 in detached leaf assays and glasshouse inoculation experiments. Finally, Koch's postulates were established by re-isolation and re-identification of the pathogen from the inoculated leaves. This work is the first to confirm a new leaf blight disease of P. deltoides caused by C. populi sp. nov. in India and worldwide., (© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)

The Hindon River is the main tributary of river Yamuna and it is a significant source of surface water, which flows through the major cities of western Uttar Pradesh, India. The indiscriminate development of industries and urbanization along river basin coupled with rapid population growths contribute various amounts of pollutant in the river. Therefore, the present study was conducted to assess the spatial-temporal variability of river water quality (seventeen physicochemical parameters and eight heavy metals) during pre- and post-monsoon seasons for 5 years data at 19 sampling sites along the river stretch. Indices associated with water quality and heavy metals were computed to scale the accurate state of risk associated to its use for drinking and irrigation. During the pre- and post-monsoon seasons, only four sites were found having safe water quality index (WQI) values. The mean heavy metal concentrations are found in order of Zn > Fe > Pb > Cu > Cr > Cd > Ni > Mn. Considering the spatial and temporal distribution, the study benchmarked the water quality of Hindon River for priority attention., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The intensity and frequency of diverse hydro-meteorological disasters viz., extreme droughts, severe floods, and cyclones have increasing trends due to unsustainable management of land and water resources, coupled with increasing industrialization, urbanization and climate change. This study focuses on the forecasting of drought using selected Artificial Neural Network (ANN)-based models to enable decision-makers to improve regional water management plans and disaster mitigation/reduction plans. Four ANN models were developed in this study, viz., one conventional ANN model and three hybrid ANN models: (a) Wavelet based-ANN (WANN), (b) Bootstrap based-ANN (BANN), and (c) Wavelet-Bootstrap based-ANN (WBANN). The Standardized Precipitation Evapotranspiration Index (SPEI), the best drought index identified for the study area, was used as a variable for drought forecasting. Three drought indices, such as SPEI-3, SPEI-6 and SPEI-12 respectively representing "short-term", "intermediate-term", and "long-term" drought conditions, were forecasted for 1-month to 3-month lead times for six weather stations over the study area. Both statistical and graphical indicators were considered to assess the performance of the developed models. For the hybrid wavelet model, the performance was evaluated for different vanishing moments of Daubechies wavelets and decomposition levels. The best-performing bootstrap-based model was further used for analysing the uncertainty associated with different drought forecasts. Among the models developed for drought forecasting for 1 to 3 months, the performances of the WANN and WBANN models are superior to the simple ANN and BANN models for the SPEI-3, SPEI-6, and SPEI-12 up to the 3-month lead time. The performance of the WANN and WBANN models is the best for SPEI-12 (MAE = 0.091-0.347, NSE = 0.873-0.982) followed by SPEI-6 (MAE = 0.258-0.593; NSE = 0.487-0.848) and SPEI-3 (MAE = 0.332-0.787, NSE = 0.196-0.825) for all the stations up to 3-month lead time. This finding is supported by the WBANN analyze uncertainties as narrower band width for SPEI-12 (0.240-0.898) as compared to SPEI-6 (0.402-1.62) and SPEI-3 (0.474-2.304). Therefore, the WBANN model is recommended for the early warning of drought events as it facilitates the uncertainty analysis of drought forecasting results., (© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Lilium mackliniae Sealy is a species endemic to the Indo-Myanmar Biodiversity Hotspot with confined occurrence in the Shirui hills of Manipur (India). A rapid fragmentation of the species resulted in enlisting the remaining population as endangered. Tribal communities have managed this population in national parks for in situ conservation because of its sacred value. Maximum entropy-based modeling was performed with 19 bioclimatic and six geospatial variables on 65 true presence data to assess the threat on its only habitat. The accuracy of modeling was confirmed by an AUC value of 0.989. Among the variables used for habitat modeling, elevation range was a key predictor of the species distribution, followed by land use class and annual temperature range. Additionally, the model projected a similar habitat for the species in the same district. This study, therefore, provides essential information for the conservation and management of the species., (© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Coscinium fenestratum is a medicinally significant critically endangered plant found in Western Ghats of India. The leaf spot and blight was observed in Kerala during 2021 with disease incidence of 40% in 20 assessed plants in 0.6 hectare. The associated fungus was isolated on potato dextrose agar medium. A total of six morpho-culturally identical isolates were isolated and morphologically identified. Based on morpho-cultural features, the fungus was identified at genus level as Lasiodiplodia sp., which was further authentically confirmed as Lasiodiplodia theobromae by molecular identification with a representative isolate (KFRIMCC 089) using multigene (ITS, LSU, SSU, TEF1-α, and TUB2) sequence analysis and concatenated phylogenetic analysis (ITS-TEF1-α-TUB2). Pathogenicity tests were also assessed in vitro and in vivo using mycelial disc and spore suspension of L. theobromae, and the isolated fungus's pathogenic behaviour was confirmed after re-isolation and morpho-cultural features. Literature survey reveals that there are no reports of L. theobromae on C. fenestratum from all over the world. Hence, C. fenestratum is being firstly reported as a new host record for L. theobromae from India., (© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.)

Bamboo is an important genetic resource in India, supporting rural livelihood and industries. Unfortunately, most Indian bamboo taxa are devoid of basic genomic or marker information required to comprehend the genetic processes for further conservation and management. In this study, we perform genome survey sequencing for development of de novo genomic SSRs in Dendrocalamus longispathus, a socioeconomically important bamboo species of northeast India. Using Illumina platform, 69.49 million raw reads were generated and assembled into 1,145,321 contig with GC content 43% and N50 1228 bp. In total, 46,984 microsatellite repeats were mined-out wherein di-nucleotide repeats were most abundant (54.71%) followed by mono- (31.91%) and tri-repeats (9.85%). Overall, AT-rich repeats were predominant in the genome, but GC-rich motifs were more frequent in tri-repeats. Afterwards, 21,596 SSR loci were successfully tagged with the primer pairs, and a subset of 50 were validated through polymerase chain reaction amplification. Of these, 36 SSR loci were successfully amplified, and 16 demonstrated polymorphism. Using 13 polymorphic SSRs, a moderate level of gene diversity (He = 0.480; Ar = 3.52) was recorded in the analysed populations of D. longispathus. Despite the high gene flow (Nm = 4.928) and low genetic differentiation (F ST  = 0.119), severe inbreeding (F IS  = 0.407) was detected. Further, genetic clustering and STRUCTURE analysis revealed that the entire genetic variability is captured under two major gene pools. Conclusively, we present a comprehensive set of novel SSR markers in D. longispathus as well as other taxa of tropical woody bamboos., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The orthocentrine ichneumonid genera Gnathochorisis and Symplecis are reported for the first time from India with the description of two new species. Taxonomic keys for the identification of Indomalayan Gnathochorisis and Symplecis are provided along with the illustration of the new species.

Background: Aconitum heterophyllum Wall. ex Royle and Aconitum balfourii Stapf, are two highly important, threatened medicinal plants of the Indian Himalayan Region. Root-tubers of Aconites have occupied an important place in Indian pharmacopoeia from very ancient times. India is a hub of the wild-collected medicinal herbs industry in Asia and these two aconites are known to have been heavily traded from the region in illicit manner. Prosecution of these illegal trading crimes is hampered by lack of pharma-forensic expertise and tools., Methods and Results: Present study was conducted to evaluate the discriminatory potential of rbcL, a Chloroplast based DNA barcode marker for the authentication of these two Himalayan Aconites. Fresh plant samples were collected from their natural distributional range as well as raw materials were procured from herbal market and a total of 32 sequences were generated for the rbcL region. Analysis demonstrated that rbcL region can successfully be used for authentication and importantly, both the aconites, were successfully discriminated by rbcL locus with high bootstrap support (> 50%)., Conclusion: Molecular markers could certainly be relied upon morphological and chemical markers being tissue specific, having a higher discriminatory power and not age dependent. Phylogenetic analysis using Maximum Likelihood Method revealed that the rbcL gene could successfully discriminate Himalayan Aconites to species level and have potential to be used in pharma-forensic applications as well as to curb illicit trade of these invaluable medicinal plants., (© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)

Most endangered species exist today in small populations, many of which are isolated. Evolution in such populations is largely governed by genetic drift. Empirical evidence for drift affecting striking phenotypes based on substantial genetic data are rare. Approximately 37% of tigers ( Panthera tigris ) in the Similipal Tiger Reserve (in eastern India) are pseudomelanistic, characterized by wide, merged stripes. Camera trap data across the tiger range revealed the presence of pseudomelanistic tigers only in Similipal. We investigated the genetic basis for pseudomelanism and examined the role of drift in driving this phenotype's frequency. Whole-genome data and pedigree-based association analyses from captive tigers revealed that pseudomelanism cosegregates with a conserved and functionally important coding alteration in Transmembrane Aminopeptidase Q ( Taqpep ), a gene responsible for similar traits in other felid species. Noninvasive sampling of tigers revealed a high frequency of the Taqpep p.H454Y mutation in Similipal (12 individuals, allele frequency = 0.58) and absence from all other tiger populations (395 individuals). Population genetic analyses confirmed few (minimal number) tigers in Similipal, and its genetic isolation, with poor geneflow. Pairwise F ST (0.33) at the mutation site was high but not an outlier. Similipal tigers had low diversity at 81 single nucleotide polymorphisms (mean heterozygosity = 0.28, SD = 0.27). Simulations were consistent with founding events and drift as possible drivers for the observed stark difference of allele frequency. Our results highlight the role of stochastic processes in the evolution of rare phenotypes. We highlight an unusual evolutionary trajectory in a small and isolated population of an endangered species., Competing Interests: The authors declare no competing interest., (Copyright © 2021 the Author(s). Published by PNAS.)

The types specimens of many taxa described by Chandy Kurian were considered lost for many years, which prevented the recognition of the species delimited by him. Fifty holotypes have been rediscovered in the National Forest Insect Collection of Forest Research Institute, Dehradun, Uttarakhand, India. Forty-five types belong to Bethylidae (Cephalonomia tritici, Chlorepyris biharina, C. mackenziei, C. pusana, C. sanctipauli, Epyris agraensis, E. agrensis, E. bombayensis, E. delhiensis, E. duni, E. duttai, E. indicae, E. manii, E. patnae, E. pusae, E. shishami, Goniozus ahmeadi, G. chatterjii, G. chowdhari, G. hybleae, G. lygropiae, G. madrassippattanami, G. marasmi, G. mori, G. morindae, G. nephoterycis, G. nilamburensis, G. pulveriae, G. salvadorae, G. sanctijohannis, G. thalasodi, Laelius agraensis, Odontepyris argyriae, O. batrae, O. cirphi, O. hypsipylae, O. indicus, Pristepyris agraensis, Pristocera govindrami, Sclerodermus delhiensis, Sc. hardwickiae, Sc. mori, Sc. raonis, Sulcomesitius bahaduri and Su. duni), one to Chrysididae (Lustrina assamensis), three to Dryinidae (Lestodryinus guptai, Neodryinus karnalensis and N. pusae) and one to Formicidae (Neoclystopsenella luffae). Six new combinations are proposed: Epyris mackenziei comb. nov. from Chlorepyris; Formosiepyris agraensis comb. nov. from Epyris; Holepyris raonis comb. nov. from Sclerodermus; Holepyris patnae comb. nov. from Epyris; Pristocera agraensis comb. nov. from Pristepyris; and Trachepyris sanctipauli comb. nov. from Chlorepyris. We also provide updated diagnoses, as well as detailed remarks and photographs for all types rediscovered. In addition, we provide researchers worldwide with valuable taxonomic information for the Bethylidae, Chrysididae, Dryinidae and Formicidae described by Kurian. A review of Bethylidae from India and a tribute to Chandy Kurian has also been provided.

Microbial communities are considered as vital members to reflect the health of a riverine system. Among them, pathogenic and fecal indicators imply health risks involved with potability of river water. The present study explores the diverse microbial communities, distribution pattern of potential pathogens, and fecal indicators between the geographically distinct Himalayan and Peninsular river systems of India. It also inquires into the environmental factors associated with community variance and distribution pattern of microbial indicators. The application of high-throughput amplicon sequencing approach unveiled significant demarcation (p < 0.004, Anosim R = 0.62) of samples suggesting unique microbial diversities in these two river sediments. Random forest analysis revealed Desulfobulbulus, PSB&#95;M&#95;3, and Opitutus in Himalayan, while DA101, Bacillus, and Streptomyces in the Peninsular as significant contributors to develop overall dissimilarity between the river systems. Permutational multivariate analysis of variance and co-occurrence network analysis were used to study the relationships between microbial taxa and environmental factors. Amongst the various studied environmental parameters, pH, K, Ca, Mg, Ba, and Al in the Himalayan and salinity, Na, temperature, and Th in the Peninsular significantly influenced shaping of distinct microbial communities. Furthermore, the potential pathogenic genera, including Flavobacterium, Clostridium, Arcobacter, Pseudomonas, and Bacillus were highly prevalent in both the river systems. Arcobacter, Clostridium, Acinetobacter, Bacteroides, and Caloramator were the prominent fecal indicators in these river systems. Our findings provide salient information about the crucial role and interplay between various environmental factors and anthropogenic influences in framing the microbiome of the distinct river systems in India. Moreover, assessing potential pathogenic and fecal indicators suggest the public health risk associated with untreated sewage discharge into these water sources. The detection of various F/S indicators and potentially pathogenic bacteria in Himalayan and Peninsular river systems emphasize the urgent need for future monitoring and management of major riverine systems in India., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

A recent addition to the Indian herpetofauna is Roarchestes kollimalai Gowande, Ganesh Mirza 2020, a bush frog species, described based on a type series of three specimens from the Kolli hills in the lesser-known Eastern Ghats of Tamil Nadu, India (Gowande et al. 2020). The authors attempted to resolve the status of the previously ambiguously identified R. cf. leucolatus from this region (Ganesh Arumugam 2016) through an integrated approach using morphology, molecular DNA and call acoustics. Gowande et al. (2020) described the new species based on their morphology, biogeographic and acoustic data, for a shallow divergent lineage differing with 1.21.9% in the mitochondrial 16S gene from a closely related species R. charius. After a thorough reading of Gowande et al. (2020), we found serious flaws in the publication, as the authors overlooked many errors and misrepresented their own data, leaving their new taxon questionable with respect to its validity and status. In this work, despite pointing out these errors made by the authors, we also discuss the validity of their new taxon based on their results and suggest the way forward to resolving the issue. -.

The genus Salacia (Celastraceae) consists of many important medicinal plants used mainly against type II diabetes. Segregation and delimitation of species is difficult based on morphological features alone. DNA barcoding is the most effective and emerging method of molecular identification. It was reported that ITS2 has better discriminating power in the genus Salacia in comparison to other barcode loci. This paper describe the analysis of sequence and structural information of ITS2 to discriminate the species of Salacia. A total of 8 species of Salacia in South India and the available sequences in NCBI database were taken for the present study. NJ method based phylogenetic trees were constructed using MEGAX with primary sequence as well as using sequence and secondary structural information. Primary structure based phylogeny did not give much information whereas the dendrogram based on sequence and structural information was more informative to decipher the phylogeny of South Asian species of Salacia. The present study revealed some interesting facts regarding the genus. Secondary structure of the ITS2 sequence of S. chinensis reported from Kerala differs consistently from that of S. chinensis reported from other parts of India and of South Asia. Probably the S. chinensis in Kerala, India has diverged a lot from the original S. chinensis. ITS2 sequence of S. reticulata reported from Sri Lanka was identical to S. chinensis reported by other groups from Thailand and Udupi, India. The molecular level identity of ITS2 sequence of S. chinensis with S. reticulata suggest merger of the two species. ITS2 sequence of S. beddomei is only reported from Kerala, India showed it to be identical to S. macrosperma. This observation points to a mistaken identity of S. beddomei which could be elusive from Kerala. Phylogenetic trees constructed based on sequence and structural features of ITS2 suggest that the ancestor species of S.chinensis diversified in two evolutionary lines. One line leads to the present day S. chinensis and the other line further diversified and lead to the rest of the present day Salacia species., (Copyright © 2021. Published by Elsevier Ltd.)

Ethnopharmacological Relevance: Different plants are used for the treatment of various ailments and Acorus calamus L. is one such plant found in Western Himalaya. Rhizome of this plants has ethnomedicinal significance, as its rhizome is used for curing fever, pain and inflammation. An attempt has been made to alter the phytochemicals and increase its antioxidant property in a sustainable way with the help of mycorrhizal inoculation., Aim of the Study: Study of mycorrhizal (Funneliformis mosseae) impact on the biological activities and phytochemical profile of A. calamus L. rhizome and in silico studies of phytochemicals for their anti-inflammatory property., Materials and Methods: F. mosseae was mass multiplied by single spore culture and then A. calamus rhizomes were inoculated with it. Antioxidant potential of rhizome extract was observed by DPPH and FRAP assays and the phytochemical profiling was done with GC-MS analysis. For observing antimicrobial activity disc diffusion method was employed. Dominant phytochemicals α-asarone and monolinolein TMS were chosen for molecular docking studies against four receptors (4COX, 2AZ5, 5I1B, 1ALU)., Results: There was increase in antioxidant activity of rhizome extract after mycorrhizal inoculation. However, no change in antimicrobial activity was observed in the plant after mycorrhizal inoculation. The comparison in phytochemicals was observed by GC-MS analysis which showed qualitative and quantitative variation in biochemical content in plants. The phytochemical, α-asarone and monolinolein TMS showed highest docking score and least binding energy against 1ALU and 4COX respectively for anti-inflammatory activity., Conclusion: Medicinal plants are potential source of antioxidants which can be increased by mycorrhizal inoculation without addition of chemical fertilizers and also results in altering the phytochemical composition., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Populus deltoides is a fast-growing woody species possessing plethora of industrial applications. This species evolutionarily developed unisexual male and female catkin inflorescence on separate trees. Flowering usually occurs during early spring before the development of foliage, where buds appear near axils or at the extending shoots. In 2019, surveys were undertaken to study the flowering pattern of P. deltoides in the states of Punjab, Haryana, Uttar Pradesh and Uttarakhand in northern India. Interestingly, an anomalous flowering behaviour (appearance of off-season male catkins during autumn, i.e. October) was observed in a plantation trial at Kapurthala, Punjab. The male catkins were 2.7-3.1 ± 0.07 cm long and 0.3-0.5 ± 0.03 cm wide, which is significant for flowering and liberation of pollen grains. Preliminary results suggested that climatic factors, such as episodes of high or low temperature and the precipitation variation forcing the tree species to behave differently. Unearthing the climate-driven off-season flowering in other tree species alluded the stimulation of phytohormones, such as gibberellic and salicylic acid concentrations influencing the flowering time, therefore, needs further investigation in case of P. deltoides. Overall, this work provides early clues of changing climatic scenario altering the flowering pattern of a tropical forestry tree species.

Genus Trichogramma Westwood is being utilized in various biological control programme worldwide. Trichogramma spp. are egg parasitoids which lay their eggs inside the host eggs. More than 240 species are known, of which, 45 species are recorded from India. It is quite difficult to identify and differentiate among the species of Trichogramma due to smaller size. In present study, we hypothesized a methodology to identify the Trichogramma species based on potential morphometric characteristics. The males and females of five different species of Trichogramma namely, T. brassicae, T. chilotraeae, T. danaidiphaga, T. danausicida and T. dendrolimi were morphometrically analysed. A total of 33 characters in males and 27 in females were taken into consideration for morphometric identification and analysis using principal component method. It was observed that few characters of male like body length, length of flagellar hair, maximum marginal hair length of fore wing, genitalia characters including aedeagus length, distance between chelate structure and gonoforceps, and others were identified as important morphometric characters. Similarly, in females, ovipositor length, body and head length, eye width, antennal club width and number of setae in forewings were found important for taxonomic identification. Male and female of each species are clearly identified from high definition microscopic images., Competing Interests: The authors have declared that no competing interests exist.

Agriculture and forestry are the two major land use classes providing sustenance to the human population. With the pace of development, these two land use classes continue to change over time. Land use change is a dynamic process under the influence of multiple drivers including climate change. Therefore, tracing the trajectory of the changes is challenging. The artificial neural network (ANN) has successfully been applied for tracing such a dynamic process to capture nonlinear responses. We test the application of the multilayer perceptron neural network (MLP-NN) to project the future Agriculture, Forestry and Other Land Use (AFOLU) for the year 2050 for the South Asian Association for Regional Cooperation (SAARC) nations which is a geopolitical union of Afghanistan, Bangladesh, Bhutan, India, Nepal, Maldives, Pakistan and Sri Lanka. The Intergovernmental Panel on Climate Change (IPCC) and Food and Agriculture Organization (FAO) use much frequently the term 'AFOLU' in their policy documents. Hence, we restricted our land use classification scheme as AFOLU for assessing the influence of climate change scenarios of the IPCC fifth assessment report (RCP 2.6, RCP 4.5, RCP 6.0 and RCP 8.5). Agricultural land would increase in all the SAARC nations, with the highest increase in Pakistan and Maldives; moderate increase in Afghanistan, India and Nepal; and the least increase in Bangladesh, Bhutan and Sri Lanka. The forestry land use will witness a decreasing trend under all scenarios in all of the SAARC nations with varying levels of changes. The study is expected to assist planners and policymakers to develop nations' specific strategy to proportionate land use classes to meet various needs on a sustainable basis.

The contribution of land uses in sequestering soil carbon (C) is a key question of research in tropical regions, where C emissions due to land use changes, extreme rainfall events and high temperatures can aggravate the global issue of climate change. In the present study, different pools of soil organic C were assessed, from shifting cultivation lands (jhum), rubber, tea garden and coffee plantations of Tura district in Meghalaya, which is located in Northeastern region (NER) of India. To achieve this goal, different parameters such as bulk density (BD), total organic carbon (TOC) and C pools, viz., very labile (C VL ), labile (C L ), less labile (C LL ) and non-labile (C NL ) were analyzed. The results indicated significant correlations among TOC and C pools in most land uses, except for C NL pool. Tea gardens showed the highest value of TOC stock (62.75 ± 1.47 t C ha -1 ) while the lowest was found in the jhum lands (33.34 ± 5.04 t C ha -1 ). Similarly, both the active (12.03 mg g -1 ) and passive (4.60 mg g -1 ) C pools were highest under tea gardens. C VL was found to be positively correlated with TOC and active carbon pool (ACP), while C LL was also positively correlated with passive carbon pool (PCP). This study indicates the potential of tea gardens as a promising C sequestering land use, which can be promoted in jhum lands to mitigate the negative impacts of climate change., Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The ever-increasing vehicle counts have resulted in a significant increase in air pollution impacting human and natural ecosystems including trees, and physical properties. Roadside plantations often act as a first defense line against the vehicular emissions to mitigate the impacts of pollutants. However, they are themselves vulnerable to these pollutants with varying levels of tolerance capacity. This demands a scientific investigation to assess the role of roadside plantation for better management and planning for urban sprawl where selected trees could be grown to mitigate the impacts of harmful pollutants. The present study assesses the impacts of vehicular emissions on the adaptation and mitigation potential of two important roadside tree species i.e. Grevillea robusta and Mangifera indica planted along roadsides in the capital city of Uttarakhand. Uttarakhand is one of the Indian Western Himalayan State and its capital city is situated on the foothills of Himalaya. The adaptation and mitigation potential were evaluated by studying the response of pollutants on the functional traits which drive the physiology of the trees. The CO2 assimilation rate, transpiration rate, stomatal conductance, water use efficiency (WUE), air pollution tolerance index (APTI), copper and proline accumulation, dust removal efficiency (DRE), leaf thickness and cooling created by plantation were studied to evaluate the response of trees exposed to roadside traffics. To compare the influence of pollutants, traits of trees grown in a control site with few or absence of vehicular movement were compared with the roadside trees. The control site represented part of a reserve forest where human interference is controlled and human-induced activities are prohibited. The vehicular frequency was found to modulate tree characteristics. The tree characteristics representing WUE, APTI, proline and copper accumulation, leaf thickness, cooling impact, and DRE were enhanced significantly, while the decreased CO2 assimilation rate was observed near roadside trees compared to the control site. We found both of the species to perform well to be used as one of the potential species for roadside and urban greening. However, there is a need to assess the potential of other species in reference to the present study., Competing Interests: The authors have declared that no competing interests exist.

Fruiting bodies of Astraeus hygrometricus mushroom grown wild in the forests of Jharkhand, India were investigated for their proximate nutritional composition and taste imparting nonvolatile components. Fruiting bodies contained good amount of total carbohydrates (55.76%), reducing sugars (15.98%), protein (16.02%), and dietary fiber (39.78%) but were low in fat (3.5%), ash (3.8%), and energy (159.5 kcal). Fatty acids were represented by monounsaturated C18:1n9c oleic acid (4.59%) and saturated C16:0 palmitic acid (2.63%). In vitro digestibility of protein is an indicator of its availability to human body and A. hygrometricus has 33.2% in vitro digestibility. Among the minerals found (mg/100 g), potassium (K, 1930.0) was major mineral followed by calcium (Ca, 443.0), magnesium (Mg, 434.0), sodium (Na, 155.0), iron (Fe, 127.0), manganese (Mn, 16.0), and selenium (Se, 1.60). Pro-vitamin D2 (ergosterol) was also determined to be 1.09 mg/g. Analysis of soluble sugars indicated that mannitol (11.22 mg/g) was the major sugar alcohol conferring sweetness to the fruiting bodies. Among total free amino acids (8.20 mg/g), seven essential amino acids (3.9) and eight nonessential amino acids (4.3) were detected. Leucine (0.92) and tyrosine (0.98) were the major essential and nonessential amino acids, respectively. Aspartic (0.61) and glutamic acid (0.63) were also present in AH and responsible for its MSG like taste. Thus, A. hygrometricus is a good source of free essential amino acids and selenium, which is not synthesized by humans. Meaty flavor of the fruiting body of A. hygrometricus was mainly due to umami 5'-guanosine monophosphate (2.43 mg/g). Sweet taste and meaty flavor of the mushroom were due to nonvolatile taste components including soluble sugars and polyols, MSG like aspartic and glutamic acid, and umami 5'-nucleotide. Overall A. hygrometricus proved its edibility as a tasty and nutritional food.

Ochlandra Thwaites, an economically exploited bamboo genus of the Western Ghats of India is severely affected by unsustainable extraction, natural habitat destruction and endangerment of species resources. This taxonomically challenging genus consists of a genetic mixture of 10 related polyploid species that are difficult to define and classify using traditional morphology. The present study investigated the probability of DNA barcoding using seven standard barcode regions recommended by CBOL as a supplementary tool to define true species boundaries. Distance (MEGA v.6.0) and sequence similarity (TaxonDNA) based approaches highlighted the discriminatory power of psb A- trn H intergenic spacer barcode region, but did not support true species entities. Neighbour-joining and Bayesian inference trees supported the existence of morphospecies complex in seven species of the genus owing to weak reproductive barriers amongnaturally coexisting species. Morphological affinities existing within genus might have stemmed from natural interspecific hybridization events and consequent reticulate evolution in morphospecies complex of genus Ochlandra .

The genus Moringa Adans. comprises 13 species, of which Moringa oleifera Lam. native to India and cultivated across the world owing to its drought and frost resistance habit is widely used in traditional phytomedicine and as rich source of essential nutrients. Wide spectrum of phytochemical ingredients among leaf, flower, fruit, seed, seed oil, bark, and root depend on cultivar, season, and locality. The scientific studies provide insights on the use of M. oleifera with different aqueous, hydroalcoholic, alcoholic, and other organic solvent preparations of different parts for therapeutic activities, that is, antibiocidal, antitumor, antioxidant, anti-inflammatory, cardio-protective, hepato-protective, neuro-protective, tissue-protective, and other biological activities with a high degree of safety. A wide variety of alkaloid and sterol, polyphenols and phenolic acids, fatty acids, flavanoids and flavanol glycosides, glucosinolate and isothiocyanate, terpene, anthocyanins etc. are believed to be responsible for the pragmatic effects. Seeds are used with a view of low-cost biosorbent and coagulant agent for the removal of metals and microbial contamination from waste water. Thus, the present review explores the use of M. oleifera across disciplines for its prominent bioactive ingredients, nutraceutical, therapeutic uses and deals with agricultural, veterinarian, biosorbent, coagulation, biodiesel, and other industrial properties of this "Miracle Tree.", (© 2019 John Wiley & Sons, Ltd.)

Rattans, the spiny climbing palms of Arecaceae (Palmae) family exhibit high endemism to the biodiversity hot spots in India. Of the five rattan genera, Calamus is the only genus found in peninsular India with 15 of 21 species, endemic to the Western Ghats. The extensive utilization of rattans owing to their strength, durability and huge demand has resulted in depletion of their natural resources. Of the 15 endemic species, C. nagbettai is the most affected species on account of endemism, low population size and restricted distribution with fragmented populations. The present study revealed high amount of genetic diversity in the surviving scattered populations of the species using microsatellite markers. High gene flow ( N m = 1.498) observed across the populations resulted in low genetic differentiation (14%). A clear genetic admixture could be seen in Kerala as well as one of the Karnataka's populations while the remaining two populations were genetically distinct. UPGMA, PCoA and STRUCTURE analyses showed significantly different genetic composition in Kerala population compared to other populations. Kerala and Karnataka populations of C. nagbettai were also unique in their genetic structure and allelic composition. Therefore, effective management and conservation strategies have to be implemented to preserve the rarealleles with adaptive potential to protect this economically valuable Calamus species from endangerment. Over exploitation, low seed set and poor regeneration, as well as habitat fragmentation can further threaten the survival of this endemic, narrowly distributed dioecious rattan species in the Western Ghats region.

Phenacoccus solenopsis is one of the major polyphagous crop pests in India. Inadequate genomic or transcriptomic resources have limited the molecular studies in this insect despite its huge economic importance. The existing molecular sequence resources of this insect were supplemented through RNA sequencing, de novo transcriptome assembly and analysis, which generated 12, 925 CDS from 23,643 contigs with an average size of 1077.5 bp per CDS and 85.1% positive BLAST hits with NCBI Non redundant (nr) database. Twenty three genes involved in RNAi machinery identified through BLASTx search against NCBI nr database suggested the existence of robust RNAi in mealybug. RNAi in P. solenopsis was demonstrated through knockdown of IAP (Inhibitor of Apoptosis), AQP (Aquaporin), CAL (Calcitonin), VATPase (V-type proton ATPase subunit F 1), bursicon, chitin synthase, SNF7 and α-amylase by injecting sequence specific dsRNA of respective genes in adult female. Additionally, feeding RNAi has been demonstrated in 2 nd instar nymph through dsRNA uptake in plant. The knockdown of core RNAi machinery genes such as Dicer, Argonaute and Staufen significantly hampered RNAi efficiency in this insect. However, downregulation of dsRNases improved RNAi efficiency. Sequential studies for understanding RNAi in P. solenopsis using transcriptome sequences have also been reported. The present study provides a base for future research on developing RNAi as strategy for management of this pest.

Industrialization and globalization have resulted in pollution of all the three ecosystems, including soil, water, and air. Among these, air pollution has generated much interest, since it has a major influence on the transboundary dispersion of pollutants globally. Air pollution tolerance index (APTI) value represents tolerance level of plants which help in selecting the most suitable plant species for plantation in/around affected areas. This parameter in conjunction with Anticipated Performance Index (API) can provide a logical solution for green belt development by considering biological and socio-economic aspect of the species and help in reducing the levels of pollutants. The present study was conducted in Himachal Pradesh, constituting a very vital part of the Indian Himalayan Region. In the present study, APTI and API values of six commonly growing temperate and sub-temperate plant species viz., Quercus leucotrichophora, Rubus ellipticus, Debregeasia saeneb, Hypericum oblongifolium, Punica granatum, and Grevillea robusta, were evaluated along the National Highway-5 in Himachal Pradesh. The highest value of APTI was observed for Grevillea robusta (12.89), followed by Punica granatum (10.87), Debregeasia saeneb (10.50), Hypericum oblongifolium (10.43), Rubus ellipticus (10.18), and Quercus leucotrichophora (9.68). Upon assessment of API, it was observed that Grevillea robusta (62.50%) was the highest scoring plant species in trees, while Rubus ellipticus and Debregeasia saeneb were the highest scoring shrub species (56.25% each) and thus can be recommended for green belt development and attenuation of air pollution in the region. Punica granatum can be suggested for plantation among the native species.

Type specimens of 28 species of Chrysomelidae and one of Megalopodidae present in National Forest Insect Collection (NFIC), Forest Research Institute (FRI), Dehra Dun (India) are digitized. Information such as label data, original citations, photographs of specimens from dorsal side, and condition of specimens are provided. These types (4 primary and 25 secondary) belong to 20 genera, i.e. 19 in 12 tribes of 5 subfamilies of Chrysomelidae-Bruchinae (Spermophagus atroapicalis Pic, S. notatipennis Pic, S. uniformis Pic, Bruchus bilineatopygus Pic, B. gardneri Pic, B. albocallosus Pic, and B. maculatithorax Pic), Cassidinae (Callispa almora Maulik, Downesia sasthi Maulik Estigmena bicolor Bhasin, and Platypria garthwaitei Bhasin), Cryptocephalinae (Aetheomorpha coorgensis Bryant, Aspidolopha nigrocurvilinea Bryant, A. ornaticollis Bryant, Chlamys padmanabhai Bhasin, C. travancorensis Bhasin, Exema fulvitarsis Bryant, E. salemensis Bhasin, Melixanthus coorgensis Bryant, and Gynandrophthalma flaviventris Bryant), Eumolpinae (Trichotheca beesoni Bryant), Galerucinae (Chaetocnema merguiensis Bryant, Diorhabda trirakha Maulik, Elytropachys sandalensis Bryant, Longitarsus cyanipennis Bryant, L. taklechensis Bryant, Podagrica apicefulva Bryant and Sastra mamaya Maulik), and one in Megalopodidae Megalopodinae (Temnaspis quadriplagiatus Bryant).

DNA barcoding enables precise identification of species from analysis of unique DNA sequence of a target gene. The present study was undertaken to develop barcodes for different species of the genus Dalbergia, an economically important timber plant and is widely distributed in the tropics. Ten Dalbergia species selected from the Western Ghats of India were evaluated using three regions in the plastid genome (matK, rbcL, trnH-psbA), a nuclear transcribed spacer (nrITS) and their combinations, in order to discriminate them at species level. Five criteria: (i) inter and intraspecific distances, (ii) Neighbor Joining (NJ) trees, (iii) Best Match (BM) and Best Close Match (BCM), (iv) character based rank test and (v) Wilcoxon signed rank test were used for species discrimination. Among the evaluated loci, rbcL had the highest success rate for amplification and sequencing (97.6%), followed by matK (97.0%), trnH-psbA (94.7%) and nrITS (80.5%). The inter and intraspecific distances, along with Wilcoxon signed rank test, indicated a higher divergence for nrITS. The BM and BCM approaches revealed the highest rate of correct species identification (100%) with matK, matK+rbcL and matK+trnH-psb loci. These three loci, along with nrITS, were further supported by character based identification method. Considering the overall performance of these loci and their ranking with different approaches, we suggest matK and matK+rbcL as the most suitable barcodes to unambiguously differentiate Dalbergia species. These findings will potentially be helpful in delineating the various species of Dalbergia genus, as well as other related genera.

A new thrips species of the grass-inhabiting Anaphothrips genus-group, Tameothrips arundo sp. n., is described from specimens collected on Arundo donax (Poaceae) from high altitude in Himachal Pradesh State of India. Four species of thrips in two subfamilies of family Thripidae are also reported for the first time from India, all of them well outside their known range: Helionothrips aino Ishida and Panchaetothrips noxius Priesner in the Panchaetothripinae, and Parabaliothrips takahashii Priesner and Scirtothrips kenyensis Mound in the Thripinae. This is the first record of Parabaliothrips Priesner from India. Diagnostic features and data on material studied for these species are provided.

The genus Acanthaleyrodes Takahashi is reported for the first time from India. Acanthaleyrodes elevatus sp. n. is described from Bridelia retusa in Kerala, India, with a key to puparia of Acanthaleydes species. The new species differs in its exceptionally elevated eighth abdominal tergite, circular vasiform orifice and eight pairs of subdorsal setae. The generic characteristics of Acanthaleyrodes are redefined and distinguished from those of Tuberaleyrodes Takahashi. A. styraci Takahashi is re-described in detail with illustrations of puparia and immatures from Hong Kong. A lectotype puparium is designated for A. callicarpae Takahashi. The development of tuberculate setae is discussed, in whitefly puparia and earlier nymphal instars, and considered to be subject to environmental modification.

A new genus of Encyrtidae (Hymenoptera: Chalcidoidea), Noyesencyrtus Singh gen. nov. (type species N. brachyoculus Singh sp. nov.), associated with insects inhabiting fruiting bodies of wood-decaying fungi, and two new species, Psyllaephagus kundapurensis Singh sp. nov. and Ooencyrtus hayatii Singh sp. nov., are described from the Western Ghats of Karnataka, India.

To optimize nursery practices for efficient plant production procedures and to keep up to the ever growing demand of seedlings, identification of the most suitable species of arbuscular mycorrhizal fungi (AMF), specific for a given tree species, is clearly a necessary task. Sixty days old seedlings of Neem (Azadirachta indica A. Juss) raised in root trainers were inoculated with six species of AMF and a mixed inoculum (consortia) and kept in green house. Performances of the treatments on this tree species were evaluated in terms of growth parameters like plant height shoot collar diameter, biomass and phosphorous uptake capabilities. Significant and varied increase in the growth parameters and phosphorous uptake was observed for most of the AMF species against control. Consortia culture was found to be the best suited AMF treatment for A. indica, while Glomus intraradices and Glomus mosseae were the best performing single species cultures. It is the first time in the state of Gujarat that a wide variety of AMF species, isolated from the typical semi-arid region of western India, were tested for the best growth performance with one of the most important tree species for the concerned region.

Aquatic biodiversity is one of the most essential characteristics of the aquatic ecosystem formaintaining its stability and a means of coping with any environmental change. The entire stretch of the Mothronwala swamp has rich riparian vegetation for providing conducive environment for the growth of aquatic organisms. The present work has been undertaken to study the bio-physico-chemical characteristics of the swamp. The data on physico-chemical environmental variables (temperature, total dissolved solutes, size and composition of substratum, pH, dissolved oxygen, alkalinity chlorides, and hardness) have been given under the present contribution. A total of 16 genera of aquatic insects belonging to orders Trichoptera, Coleoptera, Hemiptera, Ephemeroptera, Odonata and Phylum Mollusca represented the macroinvertebrates of Mothronwala swamp. The fresh water swamp of Mothronwala is under threat due to human interference and other anthropogenic activities. Some of the natural and anthropogenic environmental problems of the Mothronwala swamp have been identified and the ameliorative measures for the protection of aquatic environment and the conservation measures for the swamp have been suggested. The qualitative study revealed the present status of the aquatic biodiversity of the swamp and also about the physico-chemical parameters, which would be very helpful for policy makers to take precautionary measures to save the swamp.

The aim of this paper is to present equation for estimating potential stand density and basal area projection model for unthinned pure even-aged plantations of Eucalyptus hybrid in Gujarat State of India. Relationships between quadratic mean diameter and stems ha(-1) were developed, which was used to establish the limiting density line. Eight different stand level models, belonging to the path invariant algebraic difference form of a non-linear growth function, were compared for projecting basal area. They can be used to predict future basal area as a function of stand variables like dominant height and stem number per hectare and are crucial for evaluating different silvicultural treatment options. The performance of the models was evaluated using different statistical criteria. The model proposed by Pienaar and Shiver performed best and hence is recommended for projecting the basal area in E. hybrid stands.

Growth modelling is an essential prerequisite for evaluating the consequences of a particular management action on the future development of a forest ecosystem. Mathematical growth models are not available for many tree species in India. The objectives of this study were to estimate potential stand density and model the actual tree density and basal area development in pure even-aged stands of Eucalyptus camaldulensis. Relationships between quadratic mean diameter and stems ha(-1) were developed, and parameter values of this relationship were used to establish the limiting density line. Two different models were compared to describe the natural decrease of stem number. The model including site index as one of the variables performed slightly better than the model without site index. Seven different stand level models also were compared for predicting basal area in the stands. The models tested in this paper belong to the path invariant algebraic difference form of a nonlinear model. They can be used to predict future basal area as a function of stand variables like initial basal area, age or dominant height, and stems ha(-1) and are crucial for evaluating different silvicultural treatment options. The performance of the models for basal area was evaluated using different quantitative criteria. Among the seven models tested, the two models proposed by Pienaar and Shiver and Forss et al. had the best performance. The equations proposed to predict future basal area and stem number are related and, therefore, simultaneous regression technique has also been used to investigate the differences between parameter coefficients obtained by fitting the equations separately and jointly.

The species of Ganoderma recorded from India as causing diseases of perennial crops are listed, and their host range and taxonomy discussed. Four new hosts of G. lucidum are also reported. A decline in productivity and the death of trees are the main economic impacts due to Ganoderma diseases, and the fungus is identified as a serious pathogen of cash crops, forest plantations and trees in natural forests in the country. Ganoderma diseases have been recorded on 144 hosts in India, the major pathogens being G. lucidum and G. applanatum. G. lucidum has been recorded on 91 hosts, and appears to cause the most widespread diseases. Identification has largely been made from morphological and cultural characters, and the names currently in use should therefore be treated with caution. Cultural methods of disease control are largely inefficient in minimising inoculum pressure and in reducing the disease incidence. Chemical methods in combination with soil amendments form short-term solutions for managing the disease and improving productivity. The immediate priorities for developing an efficient management system for Ganoderma diseases in India are: (1) a thorough understanding of the etiology and epidemiology of the diseases on different hosts, (2) clarifying current ambiguity in species names, (3) assessing the inter-relationships between populations of Ganoderma on different hosts and (4) developing tools for early detection of diseases in important crops.

A silvi-herbal trial was conducted in the hot arid region of India to study the performance of a shrub (Cassia angustifolia) in combination with different tree species. The study area was frequented by frost. Leaf yield of C. angustifolia under different treatments was estimated. It was found that the shrubs produced a significantly higher yield of leaves in the vicinity of the tree species as compared to the shrubs at a far distance from the trees. This was perhaps because of more protection of crops near the canopy of the plants during frosts. The yield was not much affected by the variation of tree species indicating that the effect of tree-shrub combination was not profound. C. angustifolia as inter-crop provides support to the farming system by way of conferring stability and generating assured income.

Eucalyptus camaldulensis forms a major portion of irrigated plantations in the hot desert of India and for effective management of these plantations, growth and yield functions are necessary. This paper describes the development of height, diameter and volume growth functions based on the analysis of the data collected from the permanent sample plots laid out in the study area. The Chapman-Richards equation could be used for the prediction of diameter growth while the Schumacher model could be considered for predicting volume growth. Some base-age variant and invariant site-index models, already reported in the literature, were compared in terms of relative accuracy and it was found that the Payandeh and Wang model performed the best among the four models tried.