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The investigations were conducted at Sardar Vallabhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India during March 2019 to January 2020 for isolation and characterization and identification of phosphate solubilizing microbes (PSM) in soils of northern region of India. Soil samples (0-30 cm) were collected from four locations i.e. KVK, Lohaghat, SVPUAT, Meerut, College of Agriculture field, SVPUAT, Meerut and KVK, Bulandshahar covering Uttar Pradesh and Uttarakhand states of India and kept in refrigerator for analysis. The isolation of two rhizobial bacteria isolates (S1 and S2) in conjunction with the two-phosphate solubilizing efficient fungal isolates (F1 and F2) was carried out by the serial dilution method. The study of their morphological and microscopic characters, together with Solubilization Efficiency (SE), and Solubilization Index (SI) were also assessed. Finally, the biochemical tests were run according to their nature that was gram-negative rod by using KB002 TM HI Assorted Biochemical Test Kit (for Gram-negative rods). Between the two bacterial isolates was S1 was showing more solubilization on Pikovskaya agar (PVK) compared to S4 on the 5th day of observation, while S4 gave better results on the 8th-day solubilization observation. The biochemical tests on bacterial isolates were showing disagreement between the two, proving them as completely different isolates. In the case of fungi, the F2 isolate was showing better SE and SI values compared to the F1 isolate. [ABSTRACT FROM AUTHOR]