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Detoxification of insecticides involving major metabolic/detoxifying enzymes and target-site insensitivity is among the two most important mechanisms of biochemical resistance. Variation in the enzymatic activity highlights upon the prevalence of insecticide resistance pattern in different regions. As such, in order to study the insecticide resistance pattern in the lymphatic filariasis vector, Cx. quinquefasciatus Say (Diptera: Culicidae), field populations were collected from four districts of West Bengal, India, and quantitative enzyme activity of three major detoxifying enzymes was assessed. The enzyme activity of field collected populations was higher than that of the laboratory reared susceptible population and also differed among the study sites. Highest esterases activity was recorded in HCP (Harishchandrapur) of Malda district. DLK (Dalkhola) in Uttar Dinajpur district with a value of 3.016 nM mg protein−1 showed highest monooxygenases activity and MEK (Mekhliganj) showed highest GSTs (glutathione-S-transferases) activity (70.20 × 10–4 mM mg protein−1 min−1). This suggests varying insecticide resistance patterns in most of the studied populations. The finding of this study will be helpful in selecting an effective mosquito control strategy in the endemic districts during outbreaks. [ABSTRACT FROM AUTHOR]