1. A Medicinal Plant Extract of Scutellaria Baicalensis and Acacia Catechu Reduced LPS-Stimulated Gene Expression in Immune Cells: A Comprehensive Genomic Study Using QPCR, ELISA, and Microarray.
- Author
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Tseng-Crank, Julie, Sung, Sookyung, Jia, Qi, Zhao, Yuan, Burnett, Bruce, Park, Dae-Ryoung, and Woo, Sung-Sick
- Subjects
FAT cells ,LIPID metabolism ,ANALYSIS of variance ,ANIMAL experimentation ,CELL death ,CELL physiology ,COMPUTER software ,ENZYME-linked immunosorbent assay ,FISHER exact test ,FLAVONOIDS ,GENE expression ,HERBAL medicine ,IMMUNE system ,CHINESE medicine ,METABOLISM ,NONSTEROIDAL anti-inflammatory agents ,OXIDOREDUCTASES ,POLYMERASE chain reaction ,RESEARCH funding ,TISSUE culture ,TRADITIONAL medicine ,TUMOR necrosis factors ,BIOCHIPS ,PLANT extracts ,CYCLOOXYGENASE 2 ,DATA analysis ,REVERSE transcriptase polymerase chain reaction ,BLOOD ,PHARMACODYNAMICS ,PHYSIOLOGY - Abstract
A standardized, combined flavonoid extracts of Scutellaria baicalensis and Acacia catechu, UP446, demonstrates favorable anti-inflammatory properties. In this study, DNA microarray, quantitative polymerase chain reaction (QPCR), and enzyme-linked immunosorbent assay (ELISA) were used to study the effect of UP446 on the lipopolysaccharide (LPS)-induced pro-inflammatory gene regulation of both animal and human immortalized cell lines and also primary human cells. One consistent result from microarray was that the gene expression levels stimulated or suppressed by LPS were returned to normal levels by the UP446 co-treatment. This normalization effect from UP446 was also shown for pro-inflammatory genes cyclooxygenase (COX)-2, tissue necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 using QPCR, and TNF-α using ELISA. The controlling transcriptional factor of these genes, NFκB, was also down-regulated by UP446 in the LPS-induced cell models. Microarray analysis for numerous genes, including cytokines, chemokines, receptors, transcriptional factors, caspase, growth factors, and phosphatases, suggests not only a genomic anti-inflammatory activity for UP446 but also signaling pathways of cell proliferation, cell death, and lipid metabolism demonstrated on different types of cells. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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