1. Phytoaccumulation of iron by callus tissue of Clerodendrum indicum (L).
- Author
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Mukherjee, Anindita, Bandyopadhyay, Abhijit, Dutta, Sikha, and Basu, Sukalyan
- Subjects
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ASTHMA treatment , *IMMUNOLOGIC diseases , *IRON , *LAMIACEAE , *MEDICINAL plants , *PLANT cells & tissues , *PLANT extracts , *PLANT growth , *THERAPEUTICS - Abstract
Clerodendrum indicum(L.) is one of the important medicinal plants, originating in India. The plant is used as medicine for bronchitis, asthma and different immunological disease. Micro-propagation is a quick method, producing a huge number of plants. Apical leaf ofClerodendrum indicum(L.) was used as an explant for callogenesis. The combinations of different plant growth regulators (2,4-D, NAA, KN) in MS (Murashige and Skoog) medium produced two types of calli: one was friable and loose and the other was green, embryogenic and compact. Heavy metals cause pollution and health hazards. The plant absorbs heavy metals, which is very useful for controlling environmental pollution. Iron is a major nutrient for both plants and animals, but hyper accumulation of iron is injurious to health. Calli were used to study the optimum conditions of iron uptake in presence of different chemical environments. The different environments were created by using different chemical reagents such as (HCL, HNO3, H3PO4and KNO3). Iron (III) was spiked with radioactive Fe-59 and dynamics of uptake was followed by measuring radioactivity by gamma-ray spectrometer. The best condition of iron uptake corresponded to 1 mol Lā1KNO3after 4 hour of equilibration. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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