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The Convallaria keiskei , a plant species indigenous to Japan, is on the verge of extinction. In the past, they have been manually protected and managed. Unmanned aerial vehicles (UAVs), which are already being applied in various fields, such as agriculture, surveying, and logistics, can be applied to automate this task. Image processing and machine learning techniques applied on images obtained from UAVs can automate Convallaria keiskei classification, help to estimate the increase in colony numbers, and reduce the detection cost. In a previous study, a flower number estimation method that combines image processing and a convolutional neural network (CNN) was proposed. However, leaf regions similar to flower regions were misidentified as flower regions, and the accuracy was reduced. Therefore, in this study, a method was investigated to reduce the number of false positives by excluding areas similar to the flower regions. Specifically, a novel detection method combining image processing, CNN, and fuzzy c-means is proposed. To validate the proposed method, it was compared with the previous method as well as the method in which k-means was used instead of fuzzy c-means. All results were evaluated using flower distribution maps marked by field workers. The proposed method improved the F-measure by up to 22.0% compared with the previous method. Application of the proposed method to orthorectified images facilitates the understanding of flower populations over a wide range of areas, which can contribute to the conservation and management of the species. • Detection method for Convallaria keiskei (CK) colonies by combining CNN and FCM. • Detection CK colonies with reducion the number of false positives. • Combining method of CNN and FCM can improve the accuracy of CK colonies detection. [ABSTRACT FROM AUTHOR]

Background: Although the circumstances surrounding falls among community-dwelling elderly people have been described by numerous studies, there are few that have reported falls in the northern regions of Japan, such as Hokkaido, that experience a severe winter with heavy snow. As a preliminary study, we surveyed the circumstances of falls among community-dwelling elderly people in Hokkaido with a self-administered questionnaire. Methods: Residents living in private homes throughout Hokkaido were randomly contacted by telephone and those aged over 65 years were recruited for this study. In all, 1000 subjects participated in the survey. A questionnaire was used to obtain the histories of falls, including the number of falls, the season and place of occurrence, and the cause of the falls in the year prior to the survey. Results: Of the 1000 subjects, 849 (436 men and 413 women; mean age, 73.0 years) completed the questionnaire. Among the 849 subjects, 277 (32.6%) fell at least once during the year of the survey. Of these 277 subjects, 155 subjects (56.0%) experienced two or more falls in the year. Approximately 60% of the falls occurred during the winter. The most common location where falls occurred was on roads or sidewalks, and slipping was the most prevalent cause. Conclusion: Our findings revealed several aspects regarding the circumstances surrounding falls among community-dwelling elderly people in Hokkaido. [ABSTRACT FROM AUTHOR]

The article discusses a study on the antimicrobial susceptibility of Escherichia (E.) coli isolates from wild mice collected from a forest of Nopporo Shinrin Kouen Prefectural Natural Park in Hokkaido, Japan. It is said that antimicrobial-resistant bacteria stands as a public health problem, adding that antimicrobial agent use is regarded as the most important factor in the selection and dissemination of such bacteria. Under the study, several wild mice have been captured, species of which include the Voles, and the large and small Japanese field mouse. Results indicate that the E. coli isolates gathered from the captured wild mice rarely exhibited antimicrobial resistance.

Abstract: In the present study, we investigated the possible tick vectors that can transmit Theileria orientalis in eastern Hokkaido, Japan. Questing ticks collected from three different districts, Taiki, Otofuke, and Shin-Hidaka, of Hokkaido included Ixodes persulcatus, Haemaphysalis megaspinosa, Haemaphysalis douglasi, and Ixodes ovatus, while all the ticks collected from Yonaguni island of Okinawa were identified as Haemaphysalis longicornis. When the ticks were screened by polymerase chain reaction (PCR) for T. orientalis, the parasite was commonly detected among all tick species. Genotype-specific PCR assays revealed that all tick species in Hokkaido were predominantly detected with type 2, while ticks collected from Okinawa (H. longicornis) were predominantly detected with type 1. Consistent with the genetic diversity of T. orientalis in ticks, genotyping PCR assays from cattle grazed in the same Hokkaido sampling locations identified type 2 as the most prevalent genotype. This study provides the first identification of I. persulcatus, H. megaspinosa, H. douglasi, and I. ovatus as possible tick vectors of T. orientalis, and finds that the variety of vectors apparently capable of transmitting T. orientalis is wider in Japan than expected. The authors suggest that tick control strategies should be modified in Hokkaido based on the seasonal activities of ticks identified in the present study. [Copyright &y& Elsevier]

The genotypes of photoperiod response genes Ppd-B1 and Ppd-D1 in Japanese wheat cultivars were determined by a PCR-based method, and heading times were compared among genotypes. Most of the Japanese wheat cultivars, except those from the Hokkaido region, carried the photoperiod-insensitive allele Ppd-D1a, and heading was accelerated 10.3 days compared with the Ppd-D1b genotype. Early cultivars with Ppd-D1a may have been selected to avoid damage from preharvest rain. In the Hokkaido region, Ppd-D1a frequency was lower and heading date was late regardless of Ppd-D1 genotype, suggesting another genetic mechanism for late heading in Hokkaido cultivars. In this study, only 11 cultivars proved to carry Ppd-B1a, and all of them carried another photoperiod-insensitive allele, Ppd-D1a. The Ppd-B1a/Ppd-D1a genotype headed 6.7 days earlier than the Ppd-B1b/Ppd-D1a genotype, indicating a significant effect of Ppd-B1a in the genetic background with Ppd-D1a. Early-maturity breeding in Japan is believed to be accelerated by the introduction of the Ppd-B1a allele into medium-heading cultivars carrying Ppd-D1a. Pedigree analysis showed that Ppd-B1a in three extra-early commercial cultivars was inherited from 'Shiroboro 21' by early-heading Chugoku lines bred at the Chugoku Agriculture Experimental Station. [ABSTRACT FROM AUTHOR]