1. Phylogenetic Relationship Analysis of the Complete Genomes of Porcine Circovirus Type 2 (PCV2) Strains Isolated from Hainan Province.
- Author
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Baoguo YE, Xinli ZHENG, Yan ZHANG, Zhemin LIN, Feng WANG, and Zongxi CAO
- Subjects
PHYLOGENY ,CIRCOVIRUS diseases ,VIRUS diseases in swine ,POLYMERASE chain reaction ,SWINE - Abstract
[Objective] This study aimed to investigate the molecular characteristics of porcine circovirus type 2 (PCV2) strains isolated from Hainan Province. [ Method] The complete genome of PCV2 was amplified from PMWS-suspected samples by PCR for sequence analysis. [ Result] A total of eight PCV2 strains were isolated and identified. All the eight isolates belonged to genotype PCV2b, among which seven isolates belonged to subgenotype PCV2b-1C, and one isolate belonged to subgenotype PCV2b-1 A I B. 0RF2 gene of PCV2 isolates from Hainan Province was 705 bp in length, encoding 234 amino acids. Antigenic epitopes of Cap protein exhibited certain changes. Nucleotide sequences and deduced amino acid sequences of 0RF2 gene shared 95. 3% - 99. 7% and 93. 6% - 100% similarities among eight PCV2 isolates from Hainan Province, respectively. Moreover, nucleotide sequences and deduced amino acid sequences of 0RF2 gene of PCV2 isolates from Hainan Province shared 91.0% - 99.9% and 91.0% - 99.6% similarities with other PCV2 strain isolated from China (AY682994, AF381175, JX945577, JX682407, AY180397), respectively; nucleotide sequences and deduced amino acid sequences of 0RF2 gene of PCV2 isolates from Hainan Province shared 90.0% - 97.0% and 88.0% - 97.9% similarities with PCV2 isolates from other countries (NC_005148, JQ994268, KJ187306, AF201307, AF454546, AY754020), respectively; nucleotide sequences and deduced amino acid sequences of 0RF2 gene of PCV2 isolates from Hainan Province shared 98.2% - 100% and 94. 9% - 100% similarities with vaccine strain SH, respectively; nucleotide sequences and deduced amino acid sequences of 0RF2 gene of PCV2 isolates from Hainan Province shared 90.6% - 91.7% and 89.7% - 91.0% similarities with vaccine strain LG, respectively. [Conclusion] This study provided theoretical basis for the prevention and control of PCV2 and selection of vaccine strains in Hainan Province. [ABSTRACT FROM AUTHOR]
- Published
- 2015