Search

In order to reveal the influence of overwintering behaviour on the flavour of Scylla paramamosain, this study selected crabs of before and after overwintering in SM (Sanmen County, Zhejiang Province), HP (Hepu County, Guangxi Province) and WN (Wanning city, Hainan Province). The contents of flavouring‐related substances in hepatopancreas, muscles and gonads were determined by high‐performance liquid chromatography (HPLC); taste activity value (TAV) and equivalent umami concentration (EUC) were calculated. The results showed that sweetish amino acids were mainly accumulated in muscles, umami amino acids were mainly accumulated in hepatopancreas, free nucleotides were mainly accumulated in gonads, and the EUC value in gonads was the highest. After overwintering, the content of sweetish, umami amino acids and free nucleotides in muscle and hepatopancreas decreased significantly, while the content of flavour‐tasting amino acids in gonads did not change significantly, but the content of umami nucleotides increased significantly. Therefore, overwintering behaviour decreased the flavour of muscle and hepatopancreas, slightly enhanced gonad and the overall flavour of S. paramamosain before overwintering was better than that after overwintering. [ABSTRACT FROM AUTHOR]

Abstract: In this paper, we analyzed population genetic diversity of Scylla paramamosain in Hainan Island of China based on COI gene sequence. Totally 92 individuals were collected from six localities: Haikou, Wenchang, Wanning, Sanya, Dongfang and Danzhou. A 761 bp fragment was sequenced, defining 32 different haplotypes. H4 was the most frequent haplotype, existing in all six localities, while the majority of haplotypes were rare ones, existing in only one or two individuals. Haplotype diversity (h) and nucleotide diversity (π) ranged from 0.625 to 0.914 and from 0.001 to 0.003, with an average of 0.841 and 0.002, respectively. Genetic distance ranged from 0.001 to 0.003 within localities and from 0.002 to 0.003 between localities. The AMOVA analysis indicated a low level of genetic differentiation among six localities (F ST = 0.0176, P > 0.05). Neutrality tests and mismatch analysis implied a potential population expansion event for this crab species. [Copyright &y& Elsevier]