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Cobweb disease is a fungal disease that can cause serious damage to edible mushrooms worldwide. To investigate cobweb disease in Morchella sextelata in Guizhou Province, China, we isolated and purified the pathogen responsible for the disease. Through morphological and molecular identification and pathogenicity testing on infected M. sextelata, we identified Cladobotryum mycophilum as the cause of cobweb disease in this region. This is the first known occurrence of this pathogen causing cobweb disease in M. sextelata anywhere in the world. We then obtained the genome of C. mycophilum BJWN07 using the HiFi sequencing platform, resulting in a high-quality genome assembly with a size of 38.56 Mb, 10 contigs, and a GC content of 47.84%. We annotated 8428 protein-coding genes in the genome, including many secreted proteins, host interaction-related genes, and carbohydrate-active enzymes (CAZymes) related to the pathogenesis of the disease. Our findings shed new light on the pathogenesis of C. mycophilum and provide a theoretical basis for developing potential prevention and control strategies for cobweb disease. [ABSTRACT FROM AUTHOR]

Morchella sextelata is an economically important edible mushroom. From February to April 2022, brown rot symptoms were observed on the stipe of M. sextelata in three mushroom farms in Guizhou Province, China. The disease incidence was approximately 10–15 %, which led to significant yield losses. We isolated and purified three pathogenic bacterial isolates and conducted morphological and biochemical tests, molecular identification, and pathogenicity tests. Based on the results, the pathogen responsible for stipe brown rot was identified as Pseudomonas tolaasii. To the best of our knowledge, this is the first report of P. tolaasii causing stipe brown rot disease in M. sextelata in China. Our findings provide insights into the identification of stipe brown rot in M. sextelata cultivation. • Morchella sextelata exhibited brown rot symptoms in Guizhou, China. • Disease incidence ranges from 10 to 15 %. • Identified causal organism was Pseudomonas tolaasii. • First report of P. tolaasii causing brown rot in M. sextelata in China. [ABSTRACT FROM AUTHOR]