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1. Site-directed enzyme-linked immunosorbent assay with a synthetic simian immunodeficiency virus SIVmac peptide identifying antibodies against the HIV-2 transmembrane glycoprotein.

Author

Norrby E, Chiodi F, Whalley A, Parks E, Nauclér A, Costa CM, Torstensson R, and Biberfeld G

Subjects
Blotting, Western, Enzyme-Linked Immunosorbent Assay, Epitopes analysis, False Positive Reactions, Guinea-Bissau, HIV-1 immunology, Humans, Predictive Value of Tests, Gene Products, env, HIV Antibodies analysis, HIV-2 immunology, Peptide Fragments immunology, Retroviridae Proteins immunology, Retroviridae Proteins, Oncogenic, Simian Immunodeficiency Virus immunology, Viral Envelope Proteins immunology, Viral Fusion Proteins
Abstract

A 23-amino-acid-long peptide (AIEKYLEDQAQLNAWGCAFRQVC) representing the transmembranous protein gp32 in SIVmac was used in site-directed enzyme-linked immunosorbent assay (ELISA) for detection of HIV-2-specific antibodies in 567 sera from Bissau, Guinea Bissau. Ninety out of the 567 sera were identified to contain HIV-2 antibodies by whole antigen ELISA and Western blot assays. The peptide ELISA correctly identified 89 out of these 90 seropositives (sensitivity 98.9%). Three sera falsely interpreted to be positive were encountered (specificity 99.4%). The HIV-2 peptide was also used for testing of 93 HIV-1-positive Swedish sera. None of these sera reacted. Site-directed serology employing synthetic peptides should be considered for application as a screening assay.

Published
1989

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