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Guangdong is a main dengue epidemic area in China and undergoes dengue outbreaks frequently. In 2014, Guangdong experienced a serious dengue outbreak with 45,224 confirmed cases and six deaths, which might affect the seroprevalence among the local population. There is evidence that dengue virus (DENV) infection during pregnancy may cause adverse outcomes. Therefore, it is important to assess the seroprevalence of DENV among Guangdong pregnant women. We aimed to survey the seroprevalence of anti-DENV antibodies among pregnant women in Guangdong and to analyze the features of different seroprofiles. We collected a total 951 samples from pregnant women living in Guangdong in 2016. All serum samples were screened for DENV-specific antibodies (IgG and IgM) by indirect enzyme-linked immunosorbent assay (ELISA) and confirmed using capture ELISA. In IgM-positive samples, we performed DENV RNA detection using reverse transcriptase polymerase chain reaction. We collected information of delivery outcomes, neonate features, and clinical laboratory variables of parturients and newborns following delivery. Seroprevalence of DENV among the women in our sample was 4.31%. A total 1.26% and 3.15% of samples were IgM and IgG positive, respectively. In addition, 22.22% of IgM-positive and 9.09% of IgG-positive participants had adverse outcomes. There was no difference with respect to adverse outcomes compared with controls (8.80%) who were IgG and IgM negative. There was no difference in clinical laboratory variables among the different seroprofiles. We found a high seroprevalence of DENV among pregnant women in Guangdong comparing with the overall seroprevalence of local population before 2014. Asymptomatic DENV infection during pregnancy was not found to contribute to adverse outcomes. [ABSTRACT FROM AUTHOR]