1. Molecular investigation of an outbreak of multidrug-resistant Acinetobacter baumannii, with characterisation of class 1 integrons.
- Author
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Kraniotaki E, Manganelli R, Platsouka E, Grossato A, Paniara O, and Palù G
- Subjects
- Acinetobacter baumannii drug effects, Acinetobacter baumannii isolation & purification, Cross Infection epidemiology, Cross Infection microbiology, Electrophoresis, Gel, Pulsed-Field, Female, Greece, Humans, Intensive Care Units, Male, Microbial Sensitivity Tests, Polymerase Chain Reaction methods, Acinetobacter Infections epidemiology, Acinetobacter Infections microbiology, Acinetobacter baumannii genetics, Disease Outbreaks, Drug Resistance, Multiple, Bacterial, Integrons genetics
- Abstract
We investigated a multidrug-resistant Acinetobacter baumannii outbreak in the Intensive Care Unit (ICU) of a tertiary care hospital in Greece over a 3-month period. Molecular typing of the outbreak isolates from 31 patients revealed that two distinct genotypes were involved. Nine isolates, belonging to both genotypes, were resistant to carbapenems. Samples from the ICU environment and from the hands of personnel were collected to identify possible contamination. Class 1 integrons of 3.1, 2.5 and 2.2 kb were amplified from the clinical and environmental isolates. The 3.1 kb integron carrying five gene cassettes was found for the first time in A. baumannii. The outbreak ceased after implementation of hygienic measures in the ICU, including complete cleaning and disinfection.
- Published
- 2006
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