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Background: Microbubbles (MBs) can serve as an ultrasound contrast agent, and has the potential for magnetic resonance imaging (MRI). Due to the relatively low effect of MBs on MRI, it is necessary to develop new MBs that are more suitable for MRI. In this study, we evaluate the properties of SonoVue® and custom-made Fe3O4-nanoparticle-embedded microbubbles (Fe3O4-MBs) in terms of contrast agents for ultrsonography (US) and MRI. Methodology/Principal Findings: A total of 20 HepG2 subcutaneous-tumor-bearing nude mice were randomly assigned to 2 groups (i.e., n = 10 mice each group), one for US test and the other for MRI test. Within each group, two tests were performed for each mouse. The contrast agent for the first test is SonoVue®, and the second is Fe3O4-MBs. US was performed using a TechnosMPX US system (Esaote, Italy) with a contrast-tuned imaging (CnTITM) mode. MRI was performed using a 7.0T Micro-MRI (PharmaScan, Bruker Biospin GmbH, Germany) with an EPI-T2 sequence. The data of signal-to-noise ratio (SNR) from the region-of-interest of each US and MR image was calculated by ImageJ (National Institute of Health, USA). In group 1, enhancement of SonoVue® was significantly higher than Fe3O4-MBs on US (P,0.001). In group 2, negative enhancement of Fe3O4-MBs was significantly higher than SonoVue® on MRI (P<0.001). The time to peak showed no significant differences between US and MRI, both of which used the same MBs (P>0.05). The SNR analysis of the enhancement process reveals a strong negative correlation in both cases (i.e., SonoVue® r =20.733, Fe3O4-MBs r =20.903, with P<0.05). Conclusions: It might be important to change the Fe3O4-MBs' shell structure and/or the imagining strategy of US to improve the imaging quality of Fe3O4-MBs on US. As an intriguing prospect that can be detected by US and MRI, MBs are worthy of further study. [ABSTRACT FROM AUTHOR]

Abstract  During the last seven years various aspects of the impact-echo method for non-destructive testing of concrete structures were studied in great detail by the authors. This work was performed within the framework of the German collaborative research initiative FOR 384, “Non-destructive evaluation of concrete structures using acoustic and electromagnetic methods”, and was supported by the German National Science Foundation (Deutsche Forschungsgemeinschaft, DFG). During this period significant new and important results were obtained and well known facts were re-discussed from a different angle of view. It is the belief of the authors that most of the new and even some of the old facts are not well-established in the impact-echo community. The main aim of the present paper therefore is to summarize these important aspects of impact-echo in terms of ten short lectures. In doing so, we will not only present results of our own work but will also include contributions from other groups with significant relevance for the practical use of impact-echo in field tests. [ABSTRACT FROM AUTHOR]

Objective: To use a non-invasive measurement of oxygen saturation in chronic leg ulcers after the application of a topical haemoglobin spray to investigate if photoacoustic tomography is able to measure the oxygen saturation and if the stimulated oxygen increase can be demonstrated., Method: We measured the oxygen saturation of the ulcer tissue in five patients with chronic leg ulcers before application and 5 and 20 minutes after application of the haemoglobin spray, using photoacoustic tomography as a new method to assess oxygenation in real-time., Results: The average oxygen saturation showed a significant increase from 56.4% before to 69% (p=0.042) after 5 minutes and 78.8% (p=0.043) 20 minutes after the topical haemoglobin application., Conclusion: The oxygenation status of chronic, hard-to-heal wounds is gaining increasing interest in modern wound therapy. Topical haemoglobin spray is a new and effective method to increase the oxygenation in the ulcer tissue, but until now the link between clinical results and the mode of action was unclear. We were able to show for the first time that the use of a topical haemoglobin spray leads to an increase in oxygen saturation in vivo using photoacoustic tomography., Declaration of Interest: Joachim Dissemond received financial support from the company SastoMed for several scientific projects as well as for lectures and as an advisor. The haemoglobin spray was provided by SastoMed GmbH (Georgsmarienhütte, Germany).