1. Proteomic differences in apple spur buds from high and non-cropping trees during floral initiation.
- Author
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Kofler, Julian, Milyaev, Anton, Würtz, Berit, Pfannstiel, Jens, Flachowsky, Henryk, and Wünsche, Jens-Norbert
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PROTEOMICS , *BUDS , *HISTONE deacetylase , *HISTONE methylation , *ORCHARDS , *DNA methylation , *TREES , *APPLES - Abstract
The cropping behavior of biennial apple (Malus × domestica Borkh.) cultivars is irregular and often follows a biennial bearing pattern with 'On' years (high crop load and inhibited floral bud formation) followed by 'Off' years (little crop load and a promoted formation of floral buds). To study proteomic differences between floral and vegetative buds, trees of the strongly alternating cultivar 'Fuji' and the regular bearing cultivar 'Gala' were either completely thinned or not thinned at full bloom to establish two cropping treatments with no ('Off') or a high ('On') crop load, respectively. Student's t -Tests indicated significant differences of protein profiles in buds from 2-year old spurs from both treatments at each sampling date. Abundance patterns of protein clusters coincided with the onset of floral bud initiation and were most noticeable in buds from 'On' trees with a decreased abundance of key enzymes of the phenylpropanoid and flavonoid pathways and an increased abundance of histone deacetylase and ferritins. Furthermore, an increased abundance of proteins involved in histone and DNA methylation was found in the buds from 'Off' trees. This study presents the first large-scale, label-free proteomic profiling of floral and vegetative apple buds during the period of floral bud initiation. Although several studies exist that address the complex developmental processes associated with the formation of floral buds in apple (Malus × domestica Borkh.) at transcriptomic level, no data is available for explaining the difference between floral and vegetative buds or biennial and regular bearing cultivars on a proteomic level. This study presents the first large-scale, label-free proteomic profiling of floral and vegetative apple buds from the two cultivars 'Fuji' and 'Royal Gala' during the period of floral bud initiation and renders possible the development of suitable biomarkers for biennial bearing in apple. [Display omitted] • Significant differences in buds from trees with no and high crop load were found at proteomic level • Decreased abundance of proteins belonging to the phenylpropanoid and flavonoid pathways in 'On' buds • Increased abundance of histone deacetylase and ferritins in 'On' buds • Increased abundance of proteins involved in histone and DNA methylation in 'Off' buds • Effects of the different abundance of proteins on the flower bud initiation process to be confirmed by epigenomic studies [ABSTRACT FROM AUTHOR]
- Published
- 2022
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