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Azoles represent the most used family of organic fungicides worldwide and they are used in agriculture to circumvent the detrimental impact of fungi on yields. Although it is known that these triazoles can contaminate the air, the soil, and the water, field data are currently and dramatically lacking to assess if, and to what extent, the use of triazoles could contaminate non-target wild vertebrate species, notably in agroecosystems. In this study, we aimed to document for the first time the degree of blood contamination of a generalist wild bird species by multiple azoles which are used for plant protection and fungi pest control in various habitats. We deployed passive air samplers and captured 118 Common blackbirds (Turdus merula) in an agroecosystem (vineyard), a protected forest, and a city in western France. We collected blood and analyzed the plasma levels of 13 triazoles and 2 imidazoles. We found that a significant percentage of blackbirds living in vineyards have extremely high plasma levels of multiple azoles (means (pg.g−1); tebuconazole: 149.23, difenoconazole: 44.27, fenbuconazole: 239.38, tetraconazole: 1194.16), while contamination was very limited in the blackbirds from the protected forest and absent in urban blackbirds. Interestingly, we also report that the contamination of blackbirds living in vineyard was especially high at the end of Spring and the beginning of Summer and this matches perfectly with the results from the passive air samplers (i.e., high levels of azoles in the air of vineyards during June and July). However, we did not find any correlation between the levels of plasma contamination by azoles and two simple integrative biomarkers of health (feather density and body condition) in this sentinel species. Future experimental studies are now needed to assess the potential sub-lethal effects of such levels of contamination on the physiology of non-target vertebrate species. [Display omitted] • Common blackbirds living in vineyards are highly contaminated by multiple azoles. • Such high plasma contamination was not found in forest and urban birds. • Birds from vineyards are contaminated during the breeding season (Spring/Summer). • Difenoconazole, fenbuconazole, tebuconazole, and tetraconazole are of primary concern. • Body condition and feather quality were unrelated to the plasma levels of azoles. [ABSTRACT FROM AUTHOR]

Future environmental variations linked to climate change are expected to influence precipitation regimes and thus drinking water availability. Dehydration can be a particularly challenging physiological state for most organisms, yet no study has examined the effect of dehydration on the functioning of the hypothalamic-pituitary-adrenal axis in wild endothermic animals, despite its central role in maintaining homeostasis. In this study, we experimentally imposed a temporary water shortage (∼20 h) on captive house sparrows in order to investigate the consequences of short-term dehydration on baseline and stress-induced corticosterone levels. As expected, water-deprived birds displayed higher plasma osmolality and haematocrit. Additionally, water-deprived birds had lower defecation rates, suggesting that the mechanisms allowing caecal water absorption may be triggered very rapidly during water deprivation. Baseline but not stress-induced corticosterone levels were higher in water-deprived birds. Taken together, these results suggest that water restriction may have critical consequences on several corticosterone-related traits such as energy budget (protein catabolism and possibly feeding reduction), enhanced mobility (to promote water acquisition) and potential responses to predators (thirst threshold overriding the acute stress response). Owing to the possible fitness consequences of such components of the day-to-day life of birds, further studies should aim at investigating the influence of future changes in precipitation regimes and drinking water availability on bird populations., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2020. Published by The Company of Biologists Ltd.)