29 results on '"ISOENZYMES"'
Search Results
2. How many Archaeolacerta inhabit the Corso-Sardinian Plate? Allozyme variation and differentiation in Archaeolacerta bedriagae (Camerano, 1885).
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Salvi, Daniele, Capula, Massimo, Bombi, Pierluigi, and Bologna, Marco A.
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LIZARD populations , *ISOENZYMES - Abstract
Archaeolacerta bedriagae is a rock lizard endemic to Corsica and Sardinia. Four subspecies have been recozied to date on the basis of morphological traits. Previous allozyme investigations revealed high genetic differentiation among populations of the species. Based on these results some authors hypothesized that more than one species of Archaeolacerta may occur on Corsica and Sardinia. In this paper we investigated allozyme variation at 19 gene loci in 5 populations belonging to all subspecies of A. bedriagae in order to study genetic differentiation among populations from Corsica and Sardinia, and to compare our results with those obtained in previous studies carried out on allozyme variation and taxonomy of the species. Low levels of genetic differentiation (average Nei's D = 0.026) and heterogeneity (mean FST = 0.147) were found comparing the A. bedriagae populations, and there was no evidence of interruption or restriction of gene flow. This is in agreement with the available molecular and morphometric data, while it is not in accordance with allozyme data reported in the previous studies. Our data do not support the hypothesis of an unrecognized criptic species of Archaeolacerta in Corsica and Sardinia, and indicate that the definitive assessment of the taxonomic status of the A. bedriagae populations requires further investigation. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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3. Hereditary fructose intolerance: Frequency and spectrum mutations of the aldolase B gene in a large patients cohort from France—Identification of eight new mutations
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Davit-Spraul, Anne, Costa, Catherine, Zater, Mokhtar, Habes, Dalila, Berthelot, Jacques, Broué, Pierre, Feillet, François, Bernard, Olivier, Labrune, Philippe, and Baussan, Christiane
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DEFICIENCY diseases , *GENETIC disorders , *ISOENZYMES , *GENETIC mutation , *POLYMERASE chain reaction , *PATIENTS - Abstract
Abstract: We investigated the molecular basis of hereditary fructose intolerance (HFI) in 160 patients from 92 families by means of a PCR-based mutation screening strategy, consisting of restriction enzyme digestion and direct sequencing. Sixteen different mutations of the aldolase B (ALDOB) gene were identified in HFI patients. As in previous studies, p.A150P (64%), p.A175D (16%) and p.N335K (5%) were the most common mutated alleles, followed by p.R60X, p.A338V, c.360_363delCAAA (p.N120KfsX30), c.324G>A (p.K108K) and c.625−1G>A. Eight novel mutations were also identified in 10 families with HFI: a one-base deletion (c.146delT (p.V49GfsX27)), a small deletion (c.953del42bp), a small insertion (c.689ins TGCTAA (p.K230MfsX136)), one splice site mutation (c.112+1G>A), one nonsense mutation (c.444G>A (p.W148X)), and three missense mutations (c.170G>C (p.R57P), c.839C>A (p.A280P) and c.932T>C (p.L311P)). Our strategy allows to diagnose 75% of HFI patients using restriction enzymatic analysis and to enlarge the diagnosis to 97% of HFI patients when associated with direct sequencing. [Copyright &y& Elsevier]
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- 2008
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4. Low genetic diversity and allozymic evidence for autopolyploidy in the tetraploid Pyrenean endemic larkspur Delphinium montanum (Ranunculaceae).
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LÓPEZ-PUJOL, JORDI, ORELLANA, MARIA RENÉE, BOSCH, MARIA, SIMON, JOAN, and BLANCHÉ, CÈSAR
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DELPHINIUM , *RANUNCULACEAE , *PLANT genetics , *ISOENZYMES , *MOUNTAINS , *AUTOTETRAPLOIDY - Abstract
Allozyme electrophoresis was conducted to survey the levels and distribution of genetic diversity in the tetraploid perennial larkspur Delphinium montanum (Ranunculaceae), which is endemic to the eastern Pyrenees of Spain and France and considered to be endangered. Seven populations were sampled, resolving 14 putative enzymatic loci belonging to eight enzymes. Banding patterns stained in gels revealed several enzymatic duplications attributable to autotetraploidy, such as the presence of both balanced and unbalanced heterozygotes and the lack of fixed heterozygosity. However, variability in D. montanum ( P = 23.8%, A = 1.48, and He = 0.082) was lower than that expected for an autotetraploid species. This, in addition to the scarcity of loci showing three or four different alleles, could indicate that this species has suffered genetic erosion through population bottlenecks, or, alternatively, that it is undergoing diploidization. © 2007 The Linnean Society of London, Botanical Journal of the Linnean Society, 2007, 155, 211–222. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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5. Differential distribution of the two cryptic species, Pomatoschistus microps and P. marmoratus, in the lagoons of southern France, with an emphasis on the genetic organisation of P. microps
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Berrebi, Patrick, Rodriguez, Pascal, Tomasini, Jean-Antoine, Cattaneo-Berrebi, Ghislaine, and Crivelli, Alain J.
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SALINITY , *LAGOONS , *ISOENZYMES - Abstract
Abstract: The presence and the distribution of two cryptic sedentary gobies, Pomatoschistus microps and Pomatoschistus marmoratus, were investigated in several lagoons stretching along the Golfe du Lion, southern France Mediterranean coast, and Corsica. Pomatoschistus microps is the only sedentary Pomatoschistus species in two shallow lagoons with large variations of salinity and temperature. In contrast, P. marmoratus, another sedentary species, can be found preferentially in a deep salty lagoon with small salinity variations. Both species occur sympatrically in the Rhone Delta and hybridise, producing fertile offspring. Using allozymes, competition, linked to fitness, is invoked to explain the distribution pattern of these sibling species. [Copyright &y& Elsevier]
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- 2005
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6. Allozyme and mitochondrial DNA analysis of French red deer (Cervus elaphus) populations: genetic structure and its implications for management and conservation.
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Hartl, G. B., Zachos, F. E., Nadlinger, K., Ratkiewicz, M., Klein, F., and Lang, G.
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RED deer , *DNA analysis , *MICROSATELLITE repeats , *FRAGMENTED landscapes , *MITOCHONDRIAL DNA , *ISOENZYMES ,REPRODUCTIVE isolation - Abstract
A total of 472 red deer, ervus elaphus, from 16 free-ranging populations in France were examined for genetic variability and differentiation at 7 enzyme loci known to be polymorphic in this species. In addiditon, 73 specimens from 14 populations were examined for mtDNA differentiation using 16 six-base cutting restriction enzymes which, on the basis of 69 restriction sites, yielded altogether 5 haplotypes showing a quite variable distribution. Genetic variability within populations was quite similar, especially as far as allozymes are concerned. However, both marker systems revealed con-siderable genetic differentiation even at a small geographic scale, possibly suggesting that habitat fragmentation has already caused genetic isolation of local populations. [ABSTRACT FROM AUTHOR]
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- 2005
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7. Isozyme Analysis and Soluble Mycelial Protein Pattern in Iranian Isolates of Several formae speciales of Fusarium oxysporum.
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Mohammadi, M., Aminipour, M., and Banjhashemi, Z.
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FUSARIUM oxysporum , *ISOENZYMES , *PROTEINS - Abstract
A total of 13 representative isolates of Fusarium oxysporum f. sp. melonis (FOM) from Iran, USA and France, eight isolates of seven formae speciales from Iran and one isolate of F. oxysporum f. sp. niveum from the USA were compared based on isozyme analysis and soluble mycelial protein pattern. Isozyme analyses of alkaline phosphatase (ALP), catalase (CAT), esterase (EST), malate dehydrogenase (MDH), superoxide dismutase (SOD) and xanthine dehydrogenase (XDH) revealed polymorphism among the F. oxysporum isolates in which 22 electrophoretic phenotypes (EP) were determined. At least 10 putative loci for these six enzymes were detected and they were all polymorphic. Maximum genetic diversity was observed in CAT, EST and XDH loci. Using UPGMA, the 22 isolates were separated into three main groups with one of the groups divided into two subgroups. Group I included isolates belonging to five formae speciales from Iran, whereas group II that included FOM isolates from both Iran and the USA was divided into two subgroups each containing the vast majority of the respective isolates from either country. Group III constituted FOM isolates from France and one pathogenic isolate on pepper from Iran. FOM isolates representing five different geographical regions from Iran belonged to two different races of 1 and 1,2Y and one vegetative compatibility group (VCG)0134 and thus were genetically homologous. Isozyme polymorphism in these isolates was highly correlated with VCG and geographical origins and to a lesser extent with races. Variations in soluble protein profile in FOM isolates were correlated with genetic distances determined in isozyme analysis. This study suggests that isozyme analysis could be a useful tool for identifying genetic diversity not only in FOM but also several formae speciales of F. oxysporum. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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8. Genetic differentiation of autochthonous populations of Pinus sylvestris (Pinaceae) from the Iberian peninsula.
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Prus-Glowacki, W., Stephan, B. R., Bujas, E., Alia, R., and Marciniak, A.
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PINACEAE , *SCOTS pine , *POPULATION genetics - Abstract
Genetic structure and genetic diversity in fourteen autochthonous populations of Pinus sylvestris L. from the Iberian peninsula and two populations from the Massif Central in France were studied at eleven isoenzymatic polymorphic loci. The studied populations formed two genetically similar but heterogeneous groups. The first group consist of populations localized in North-Eastern Spain with two genetically distinct populations. The second includes populations of the West-Central part of the Iberian peninsula. Populations from the Massif Central in France are diverse from those of Spain, but are similar to the group from North- part of the Iberian peninsula. The existence of significant numbers of private alleles in Spanish populations which were not observed in populations from the Massif Central, suggests that the Scots pine from the Iberian peninsula probably did not take part in the colonization of Europe after the last glaciations and represents original ancient tertiary gene pools. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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9. Natural and artificial secondary contact in brown trout (Salmo trutta, L.) in the French western Pyrenees assessed by allozymes and microsatellites.
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Aurelle, D., Cattaneo-Berrebi, G., and Berrebi, P.
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BROWN trout , *ISOENZYMES , *MICROSATELLITE repeats - Abstract
Analysis of allozyme polymorphism in brown trout (Salmo trutta) populations from south-western France shows that two genetically differentiated wild forms (characterized by the LDH-C1*100 and 90 alleles) introgress in this area. As allozymes could not evaluate the impact of stocking in the Atlantic basin, microsatellites have been necessary to detect the influence of hatchery fish and to confirm that the observed structure was natural. Microsatellites confirm the distinctness of the two wild forms based on allozyme loci. The situation provides a new example of secondary contact for this species in the Atlantic basin, with various levels of mixing being seen between the two population groups. The origin of these forms is discussed in the light of previous studies concerning modern and ancestral Atlantic trout (Hamilton et al, 1989) and lineages stemming from different glacial refuges (Garcia Marin et al, 1999; Weiss et al, 2000). This local analysis provides new insights in defining the evolutionary history of this species and confirms the important role of glaciation events in this history. [ABSTRACT FROM AUTHOR]
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- 2002
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10. Genetic differentiation of Anopheles claviger s.s. in France and neighbouring countries.
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Schaffner, F., Raymond, M., and Pasteur, N.
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ANOPHELES , *MYXOMATOSIS - Abstract
SummaryAn investigation of polymorphism of 11 autosomal and one sex-linked allozyme loci was made on 18 samples of Anopheles claviger Meigen (Diptera: Culicidae) from localities across France and neighbouring sites in Germany and Switzerland, plus one sample of Anopheles petragnani Del Vecchio from the French Pyrénées. Genetic differentiation between these two sibling species was confirmed (Nei genetic distance 0.33–0.44) and two genetically distinct groups of populations were identified within An. claviger. These two forms of An. claviger showed contiguous geographical distributions, Group I found across western and Central France, Group II in eastern France and nearby parts of Germany and Switzerland. The two groups were in contact in a region near the Rhone Valley where two intermediate samples were found. The taxonomic significance of this finding is discussed in the context of the recent climatic history of Europe and in relation to the vector potential of each member of the An. claviger complex. [ABSTRACT FROM AUTHOR]
- Published
- 2000
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11. Research note. Spatial differentiation of allozyme frequencies in a subdivided population of the land snail Helix aspersa.
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Arnaud, J. F., Madec, L., and Daguzan, J.
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BROWN garden snail , *ISOENZYMES , *SNAILS , *HELIX (Mollusks) - Abstract
The article discusses a study which analyzed the spatial isozyme differentiation in 14 sampled populations of the snail Helix aspersa along a transect in the polders of the bay of Mont-Saint-Michel, Western Brittany, France. Autocorrelation statistics were used to investigate the structure of the genetic variability among natural populations of H. aspersa. It showed a genetic differentiation among colonies of H. aspersa at a microgeographic scale, and low dispersal ability combined with recolonisation processes may explain the patchy genetic structure.
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- 1999
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12. Allozyme diversity and genetic structure in South-Western populations of heather, <em>Calluna vulgaris</em>.
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Mahy, G., Vekemans, X., Jacquemart, A. L., and De Sloover, J. R.
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HEATHER , *BIODIVERSITY , *GENES , *ISOENZYMES , *SPECIES diversity , *CLUSTER analysis (Statistics) - Abstract
Genetic diversity, population genetic structure and gene flow in Calluna vulgaris (L.) Hull were assessed by means of seven allozyme loci scored in 18 population from the South-Western area of the species' range. Genetic diversity was lower (HT = 0.20) than reported for long-lived widespread species but was characterized by a high number of alleles per locus (5.60 at the species level) of which more than 70% were rare. More than 95% of genetic variation was found at the intrapopulation level (GST = 0.047). High levels of past gene flow were inferred, based on the allozyme data (Nm = 5.2 from GST, Nm = 10.2 from the 'private allele' method). Calluna vulgaris exhibited several geographic patterns of genetic variation. Both cluster analysis, constructed with various genetics distances and principal components analysis showed that Spanish and Pyrenean populations were clearly different from those collected in the Massif Central and Belgium. Also, a trend for decreasing genetic diversity towards Northern population was detected. These patterns might be related to the post-glacial history of Calluna. Inaddition, it is shown that isolation by distance has played a role in the geographic shaping of genetic variation in this species. [ABSTRACT FROM AUTHOR]
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- 1997
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13. Genetic variation of the riparian pioneer tree species Populus nigra L.I. Study of population structure based on isozymes.
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Legionnet, Agnés and Lefèvre, François
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BLACK poplar , *PLANT variation , *ISOENZYMES , *GENETIC polymorphisms , *PLANT genetics - Abstract
Eight polymorphic isozyme loci were used to describe the level and organization of the genetic diversity in Populus nigra L. over 111 sites in France and among 60 additional individuals from throughout the species range. Best descriptors and estimators of gene diversity and differentiation were sought. Overall diversity level was found to be comparable to other widespread species. Differentiation at three geographical scales was low, although local allele fixations indicated limitation of gene flow. Inferences were made on the effect of the natural history of the species on the organization of neutral diversity, and recommendations for collection of genetic resources were derived. Keywords: genetic diversity; genetic resources; isozymes; population structure; Populus nigra. [ABSTRACT FROM AUTHOR]
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- 1996
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14. Stronger spatial genetic structure in recolonized areas than in refugia in the European beech.
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de Lafontaine G, Ducousso A, Lefèvre S, Magnanou E, and Petit RJ
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- Bayes Theorem, Climate Change, Cluster Analysis, DNA, Plant genetics, Ecosystem, Fagus enzymology, France, Genetic Drift, Isoenzymes, Microsatellite Repeats, Fagus genetics, Genetic Variation, Genetics, Population
- Abstract
Extant rear-edge populations located in former glacial refugia remain understudied despite their high conservation value. These populations should have experienced strong genetic drift due to their small size and long isolation. Moreover, the prolonged action of isolation by distance in refugial areas should result in stronger regional spatial genetic structure (SGS) than in recolonized areas, but empirical tests of this prediction are scarce. To fill this gap, we first used a set of 16 microsatellite markers to investigate the genetic structure of European beech in France in 65 populations from three refugial areas and one control recolonized (nonrefugial) area. Then, using the same approach, we reanalysed published isozyme data from 375 populations distributed across the entire species range. We found stronger genetic differentiation among populations in refugia than in recolonized areas. However, contrary to expectations, regional SGS was lower within refugia than within recolonized areas. Published studies presenting similar analyses suggest that our results could have generality across different biogeographical settings and types of organisms. Strong and prolonged genetic drift in refugial areas could have erased the signature of range expansions that is still visible in recolonized areas. Our results therefore suggest that Pleistocene population isolation has played a key role in increasing the genetic complexity of extant rear-edge populations., (© 2013 John Wiley & Sons Ltd.)
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- 2013
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15. The frequency of rDNA variants within individuals provides evidence of population history and gene flow across a grasshopper hybrid zone.
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Keller I, Veltsos P, and Nichols RA
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- Animals, Chromosomes, France, Gene Flow, Geography, Grasshoppers enzymology, Isoenzymes, Karyotyping, Male, Multigene Family, DNA, Ribosomal, Evolution, Molecular, Grasshoppers genetics, Hybridization, Genetic, Polymorphism, Single Nucleotide
- Abstract
In the grasshopper Podisma pedestris, units of the ribosomal DNA (rDNA) multigene family are not identical, but comprise multiple genetic variants. We surveyed this variation using a novel pyrosequencing approach. The history of the study populations is well characterized as the pattern of colonization can be inferred from the distribution of two chromosomal races that invaded from different directions after the last glacial maximum and finally met to form a hybrid zone. This knowledge of the populations' ancestry allows us to draw inferences about the rate of change in rDNA composition. The rDNA data have, in turn, been revealing about the populations' ancestry, indicating a previously unsuspected route of postglacial colonization. The two chromosomal races were found to have genetically distinctive rDNA composition, demonstrating the persistence of differences for thousands of generations. It follows that the hybrid zone represents a natural experiment in which repeated crossing and backcrossing between these different rDNA lineages has occurred for over 8000 generations. The association between chromosomal race and rDNA composition has been broken down within the zone. It therefore appears that rDNA variants move freely across the zone and are not under opposing selection pressures in the two races, as had previously been suspected.
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- 2008
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16. Crossing species' range borders: interspecies gene exchange mediated by hybridogenesis.
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Schmeller DS, Seitz A, Crivelli A, and Veith M
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- Animals, Environment, France, Gene Frequency, Genotype, Isoenzymes, Markov Chains, Monte Carlo Method, Regression Analysis, Species Specificity, Adaptation, Physiological genetics, Demography, Genetics, Population, Hybridization, Genetic, Ranidae genetics, Selection, Genetic
- Abstract
The distribution of species is limited by their ability to adapt to local environments. For adaptation by selection, genetic variability is crucial. As founder effects reduce genetic variability, extension of species' range borders is usually slow due to the reduced probability of successful colonization. However, the range limit might be extended by incorporating locally adapted genes. In western Palaearctic waterfrogs, interspecies hybrids show hemiclonal gametogenesis, are fertile and reproductively mimic one parental species. Genetic analysis, using allozyme loci, shows that they mediate gene exchange between the two parental species. Selection analysis provides evidence for local adaptation of single locus genotypes. This suggests that hybridogenesis presents a process which increases the number of neoform parental genotypes, exposing these to selection, and thereby revealing locally adapted genotypes which are essential for species range expansion.
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- 2005
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17. European mink-polecat hybridization events: hazards from natural process?
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Lodé T, Guiral G, and Peltier D
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- Animals, Conservation of Natural Resources, France, Gene Frequency, Genotype, Isoenzymes, Microsatellite Repeats genetics, Population Dynamics, Species Specificity, Ferrets genetics, Genetics, Population, Hybridization, Genetic, Mink genetics, Polymorphism, Genetic, Reproduction genetics
- Abstract
Determining the significance of hybridization events raises essential issues both in conservation and in evolutionary biology. Here, we report a genetic investigation of sympatric polecat and endangered European mink populations. Although the two species were morphologically very similar, the European mink and the polecat were easily discriminated from allozymes and microsatellites and showed a high level of private alleles (effective number of alleles: mink=1.45 and polecat=3.09). Nevertheless, the allozymic polymorphism remained lower in the European mink (4 loci, 10.5%) than in polecat (9 loci, 23.7%). Similarly, from microsatellite data, the polymorphism only reached 36% at 0.99 in the European mink; whereas in the polecat, the polymorphism reached 82% at 0.99. Natural hybridization events between two native species were detected. Because of the low fertility of hybrids, interbreeding could be regarded as producing "hybrid sink" that leads to a progressive assimilation of mink by polecat. Nonetheless, pure mink populations inhabited streams in western France, and hybridization events were only detected in areas where mink were rare and now presumed disappeared. Rather than revealing the poor efficiency of the specific recognition system, our results suggest that hybridization is associated with the scarcity of mating partners.
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- 2005
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18. Genetic isolation between two sympatric host plant races of the European corn borer, Ostrinia nubilalis Hubner. II: assortative mating and host-plant preferences for oviposition.
- Author
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Bethenod MT, Thomas Y, Rousset F, Frérot B, Pélozuelo L, Genestier G, and Bourguet D
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- Animals, Electrophoresis, Female, France, Isoenzymes, Male, Moths physiology, Reproduction physiology, Species Specificity, Artemisia parasitology, Genetics, Population, Humulus parasitology, Moths genetics, Oviposition physiology, Zea mays parasitology
- Abstract
The European corn borer, Ostrinia nubilalis Hubner, colonized maize (Zea mays L.) after its introduction into Europe about 500 years ago and is now considered one of the main pests of this crop. In northern France, two sympatric host races have been described: one feeding on maize and the other on mugwort (Artemisia vulgaris L.) and hop (Humulus lupulus L.). In a previous study, we showed that mating between the two races may be impeded by differences in the timing of moth emergence and in the composition of the sex pheromone produced by the females. In this study, we further investigated the genetic isolation of these two races using strains from the maize (Z strain) and mugwort (E strain) races selected for diagnostic alleles at two allozyme loci. In a cage containing maize and mugwort plants and located in natural conditions, mating between individuals of the same strain occurred more often than mating between males and females of the E and Z strains. In particular, we obtained no evidence for crosses between Z females and E males. We also found that females of the Z strain laid their eggs almost exclusively on maize, whereas females of the E strain laid their eggs preferentially, but not exclusively, on mugwort. These results suggest that the genetic differentiation between the two host races may also be favored by host-plant preference, one of the first steps toward sympatric speciation.
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- 2005
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19. Physiological cost of tolerance to toxicants in the European flounder Platichthys flesus, along the French Atlantic Coast.
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Marchand J, Quiniou L, Riso R, Thebaut MT, and Laroche J
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- Analysis of Variance, Animals, Atlantic Ocean, Dose-Response Relationship, Drug, Flounder genetics, Flounder growth & development, France, Gene Frequency, Genetic Markers, Genetics, Population, Genotype, Gonads pathology, Isoenzymes, Metals, Heavy analysis, Metals, Heavy toxicity, Organ Size, Body Constitution drug effects, Fertility drug effects, Flounder physiology, Genetic Variation drug effects, Water Pollutants, Chemical toxicity
- Abstract
Physiological and genetic responses of flounder Platichthys flesus populations were investigated along the French Atlantic Coast in one moderately contaminated estuary (Ster) and three contaminated estuaries (Seine, Loire and Gironde). The focus of this study was to explore the relationship between stress resistance and energetic trade-offs, in order to detect possible differential physiological capacities or performances between individuals carrying particular alleles or genotypes (allozyme data) characterised as "tolerant" or "sensitive". A general reduction of the relative fecundity, the growth rate and the condition factor was highlighted in contaminated fish populations, suggesting that survival in such polluted systems implies energetic costs for fish thus reducing the energy available for particular functions. A lower observed heterozygosity was also detected in contaminated populations with respect to the Ster, suggesting a general decrease in genetic variability in response to chemical stress (with an exception for the Seine estuary). This study confirmed the previously detected relationship between PGM 85, AAT1 95 alleles and reduced DNA damage in contaminated fish [Marchand, J., Tanguy, A., Laroche, J., Quiniou, L., Moraga, D., 2003. Responses of European flounder Platichthys flesus populations to contamination in different estuaries along the Atlantic coast of France. Mar. Ecol. Prog. Ser. 260, 273-284] and furthermore suggested that, reduced fecundity and condition factor associated to the individuals carrying the previous alleles, were also reflecting the cost of resistance to stress in polluted populations. The cost of tolerance to stress as well as the high gene flow from neighbouring populations less exposed to contamination may explain the apparently moderate increase of the suspected "tolerant" alleles in contaminated flounder populations.
- Published
- 2004
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20. Sex-biased predation by polecats influences the mating system of frogs.
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Lodé T, Holveck MJ, Lesbarrères D, and Pagano A
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- Animals, Conflict, Psychological, Electrophoresis, Starch Gel, Female, Ferrets genetics, France, Isoenzymes, Male, Telemetry, Ferrets physiology, Predatory Behavior physiology, Ranidae physiology, Sex Ratio, Sexual Behavior, Animal physiology
- Abstract
In agile frogs, Rana dalmatina, an increase in male-biased operational sex ratio and in male abundance results in the emergence of alternative male mating behaviour in the form of searching. As a consequence, females are coerced into mating with multiple males, which in turn increases the level of conflict between the sexes. Selective predation on males by the European polecat, Mustela putorius, decreases the occurrence of polyandry. In ponds visited by polecats, the sex ratio is less male biased than in ponds where polecats are absent. As a result most males call to attract females and fewer males actively search for females. Females are able to choose between calling males and mate with a single male. Thus, predation by polecats is found to influence sex ratio, male abundance and sexual conflict in a frog mating system, restricting the opportunity for multiple mating.
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- 2004
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21. Autosomal differences between males and females in hybrid zones: a first report from Barbus barbus and Barbus meridionalis (Cyprinidae).
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Chenuil A, Crespin L, Pouyaud L, and Berrebi P
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- Age Factors, Animals, Female, France, Gene Frequency, Genetic Variation, Geography, Isoenzymes, Male, Microsatellite Repeats genetics, Population Dynamics, Rivers, Chromosomes genetics, Cyprinidae genetics, Genetics, Population, Hybridization, Genetic, Selection, Genetic
- Abstract
Narrow hybrid zones are generally subjected to the action of two forces: dispersal, which tends to homogenise the hybridising taxa, and selection against hybrids, which, in contrast, produces steep clines of introgression for diagnostic markers. Although differences between sexes in dispersal abilities or in susceptibility to hybrid counterselection are common in hybrid zones, autosomal genetic differences between males and females have never been reported to our knowledge. Barbus barbus and Barbus meridionalis (Cyprinidae) form a hybrid zone along the Lergue river. By carrying out a genetic analysis of males and females in six samples from two central stations of the hybrid zone using codominant markers (six allozymes and four microsatellite loci), we revealed significant multilocus and monolocus differences between the sexes. This could reflect a genetic difference among sexes within a same cohort, caused either by a survival (or fertility) differential among sexes or by a sex-specific pattern of dispersal. Alternatively, this may be due to genetic differentiation between cohorts, since male and female barbel exhibit different maturation, growth and survival patterns leading to different age distributions among sexes, and particularly among reproducers.
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- 2004
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22. Evidence of genetic selection for growth in new recruits of a marine fish.
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Planes S and Romans P
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- Animals, Electrophoresis, France, Gene Frequency, Genotype, Isoenzymes, Mediterranean Sea, Population Dynamics, Survival Analysis, Time Factors, Genetics, Population, Models, Theoretical, Sea Bream genetics, Sea Bream growth & development, Selection, Genetic
- Abstract
Abstract A cohort of Diplodus sargus, a coastal marine fish abundant in the Mediterranean Sea, has been surveyed from its settlement following the pelagic larval stage up to 4 months of age, when the juveniles are moving to adult habitats in order to assess selective processes. We followed the mortality by looking at the decrease in population abundance and, simultaneously, the genetic structure using allozymes and the growth associated with each genotype to test for a relationship between genotype and phenotype. The recruitment survey demonstrated that 80% of individuals arrived within a single night and that they show very similar age providing a discrete pulse of new recruits that we followed for changes in survival and allele frequencies. After 4 months, there was a total mortality of 80.8%, with the disappearance of 181 of 224 fish that initially colonized the rocky barrier. The decrease in number followed a logarithmic model with a maximum decrease in the early period (first 30 days). The model derived from the 4 months of data demonstrates that most of the mortality in the cohort occurs over the first 120 days following settlement and the model predicted a final abundance of 10 individuals after 1 year. Within the same period of 4 months, we observed significant decrease in multilocus heterozygosity. Such a decrease in heterozygosity partly resulted from a purge of the Pgm-80* allele. Together with this major change in a natural population, an aquarium experiment demonstrated that individuals with Pgm-80* alleles show significantly lower growth than other new recruits. We propose that the decrease in frequency of Pgm-80* in the natural environment is the result of targeted predation that eliminates smaller individuals and therefore individuals bearing Pgm-80*. The potential metabolic effect as well as a scenario that could lead to the maintenance of polymorphism is discussed.
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- 2004
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23. Invasion dynamics of two alien Carpobrotus (Aizoaceae) taxa on a Mediterranean island: I. Genetic diversity and introgression.
- Author
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Suehs CM, Affre L, and Médail F
- Subjects
- Aizoaceae anatomy & histology, Electrophoresis, France, Genotype, Geography, Hybridization, Genetic, Isoenzymes, Population Dynamics, Reproduction physiology, Aizoaceae physiology, Demography, Flowers anatomy & histology, Genetic Variation, Plant Leaves anatomy & histology
- Abstract
This study, based on morphological and isozyme analysis, clearly discriminates two invasive Carpobrotus taxa, C. edulis and C. acinaciformis, in the Hyères archipelago off the southeastern coast of France. However, three different allelic combinations demonstrate the presence of intermediate individuals resulting from an introgression of part of the C. edulis genome into that of C. acinaciformis. Both taxa have higher than average genetic (C. edulis: P(0.95)=62.5%, A=2.25+/-0.70, H(o)=0.329+/-0.324; C. acinaciformis: P(0.95)=75%, A=2.38+/-0.42, H(o)=0.645+/-0.109) and clonal diversities (C. edulis: IP=0.37; C. acinaciformis: IP=0.48). Furthermore, C. acinaciformis has an excess of heterozygotes (F=-0.585+/-0.217), probably due to introgression. The relationship between the probability of clonal identity for two individuals and distance indicates that C. acinaciformis relies more on clonal reproduction than on sexual recruitment (seed recruitment/vegetative propagation=u/v=0.027), in contrast to C. edulis, whose probability of clonal identity did not vary with distance. The overwhelming clonal growth and high genetic diversities of C. acinaciformis and the previously recorded invasion potential for C. edulis raises concern for intensified invasion via hybridisation.
- Published
- 2004
- Full Text
- View/download PDF
24. Differential patterns of hybridization and introgression between the swallowtails Papilio machaon and P. hospiton from Sardinia and Corsica islands (Lepidoptera, Papilionidae).
- Author
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Cianchi R, Ungaro A, Marini M, and Bullini L
- Subjects
- Animals, Bayes Theorem, Butterflies physiology, DNA, Mitochondrial genetics, Female, France, Geography, Isoenzymes, Italy, Male, Biological Evolution, Butterflies genetics, Genetics, Population, Hybridization, Genetic
- Abstract
Proportions of hybridization and introgression between the swallowtails Papilio hospiton, endemic to Sardinia and Corsica, and the holarctic Papilio machaon, were characterized using nine fully diagnostic and two differentiated allozyme loci and a mitochondrial DNA marker. Very low frequencies of F1 hybrids were detected in both Sardinia (0-4%, average 1.4%) and Corsica (0-3%, average 0.5%), as well as of first generation backcrosses (B1). No F2 were observed, in agreement with the hybrid breakdown detected in laboratory crosses. In spite of this minimal current gene exchange, specimens carrying introgressed alleles were found in high proportions in P. machaon but in lower proportions in P. hospiton. Introgression apparently occurred through past hybridization and repeated backcrossing, as evidenced by hybrid index scores and Bayesian assignment tests. Levels of introgression were low (0-1%) at two sex-linked loci and mitochondrial DNA, limited (0.4-2%) at three autosomal loci coding for dimeric enzymes, and high (up to 43%) at four autosomal loci coding for monomeric enzymes. Accordingly, selective filters are acting against foreign alleles, with differential effectiveness depending on the loci involved. The low levels of introgression at sex-linked loci and mitochondrial DNA are in agreement with Haldane's rule and suggest that introgression in P. machaon proceeds mainly through males, owing to a lower fitness of hybrid females. Papilio machaon populations showed higher levels of introgression in Sardinia than in Corsica. The role of reinforcement in the present reproductive isolation between P. machaon and P. hospiton is examined, as well as the evolutionary effects of introgressive hybridization between the two species.
- Published
- 2003
- Full Text
- View/download PDF
25. [Incidence of myocardial lesions after vascular surgery: diagnosis by troponin Ic].
- Author
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Guerre-Berthelot P, Crama P, Prima F, Oddoze C, Branchereau A, Gouin F, and Auffray JP
- Subjects
- Aged, Aortic Aneurysm, Abdominal surgery, Aortic Diseases surgery, Arterial Occlusive Diseases surgery, Biomarkers, Coronary Angiography, Coronary Disease complications, Creatine Kinase blood, Electrocardiography, Female, France epidemiology, Humans, Incidence, Isoenzymes, Male, Middle Aged, Myocardial Ischemia blood, Myocardial Ischemia diagnosis, Postoperative Complications blood, Postoperative Complications diagnosis, Prospective Studies, Risk Factors, Myocardial Ischemia epidemiology, Postoperative Complications epidemiology, Troponin I blood, Vascular Surgical Procedures statistics & numerical data
- Abstract
Objective: To compare the incidence of myocardial damages diagnosed following vascular surgery using the cardiac troponin I measurement technique and conventional methods., Study Design: Prospective epidemiological study., Patients: Fifty-four patients who underwent surgery for either aneurysmal disease in 28 cases or occlusive aortic disease in 26 cases., Methods: Plasma concentration of cardiac troponin I (significant at a concentration > 1.5 ng.mL-1) was measured by immunoenzymofluorimetry on the second and fifth postoperative days. Conventional monitoring methods included daily electrocardiogram (ECG), enzymatic assay of total-PCK, and measurement of plasma levels of the MB isoenzyme of phosphokinase creatine (MB-PCK) (significant at > 1 ng.mL-1 and RI > 1.5)., Results: The cardiac troponin I measurement technique allowed the diagnosis of minor myocardial damages during the postoperative period in five patients, whereas with the conventional methods (clinical signs. ECG, and MB-PCK) only three myocardial lesions were diagnosed., Conclusion: The cardiac troponin I measurement technique allows diagnosis of minor myocardial damages following vascular surgery. Conventional methods underestimate the incidence of these damages.
- Published
- 1997
- Full Text
- View/download PDF
26. [Identification by starch gel electrophoresis of the species of lizards Lacerta muralis Laurenti 1768 and Lacerta hispanica Steindachner 1870 in the sympatric populations of Spain and Languedoc-Roussillon].
- Author
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Guillaume CP, Pasteur N, and Bons J
- Subjects
- Alleles, Animals, Aspartate Aminotransferases analysis, Aspartate Aminotransferases blood, Electrophoresis, Starch Gel, Female, France, Isoenzymes, L-Lactate Dehydrogenase analysis, Lizards blood, Male, Phenotype, Spain, Lizards classification
- Abstract
An electrophoretic analysis of three enzymatic loci (Got-1, Ldh-A and Ldh-B) has shown that examination of Got-1 alleles allows unambiguous identification of L. muralis and L. hispanica in areas where the two species are sympatric and not identifiable by classical techniques of systematics. At the Ldh-A locus, one allele is common to both species, but a second allele is L. muralis specific and a third one is L. hispanica specific.
- Published
- 1976
27. [Enzymatic and serologic study of human trichinosis. Apropos of a recent epidemic in a suburb south of Paris].
- Author
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Gentilini M, Vernes A, Gentilini JL, Richard-Lenoble D, Bourée P, and Wattez A
- Subjects
- Alanine Transaminase blood, Aspartate Aminotransferases blood, Creatine Kinase blood, Fluorescent Antibody Technique, France, Humans, Isoenzymes, L-Lactate Dehydrogenase blood, Trichinellosis enzymology, Trichinellosis immunology, gamma-Glutamyltransferase blood, Trichinellosis diagnosis
- Abstract
An enzymatic and immunologic study of 18 patients with trichinosis leads to the following conclusions: The stage of muscular invasion in trichinosis is accompanied by a release of cellular enzymes representative of striated muscle fibres in nearly all the cases. This release can be observed by a study of the LDH iso-enzymes at a time when immunological techniques are not always significantly positive. The specific aspect of this phenomenon can be proposed with reservations since there does not exist any interference with other enzymatic systems such as the gamma-GT and furthermore no other evident cause of muscular lysis is present. The existence of a blood hypereosinophilia completes the biological picture. These early modifications of the enzymatic activities are most probably transient.
- Published
- 1976
28. [Mice of southern France: genetic characterization of two groups of sympatric populations].
- Author
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Britton J, Pasteur N, and Thaler L
- Subjects
- Animals, Ecology, Esterases analysis, France, Isocitrate Dehydrogenase analysis, Isoenzymes, L-Lactate Dehydrogenase analysis, Serum Albumin analysis, Species Specificity, Genetics, Population, Genotype, Mice physiology
- Abstract
An electrophoretic survey of five proteins (lactate dehydrogenase, isocitrate dehydrogenase, esterase-1, esterase-2, albumin) shows that there are two groups of populations strongly (or completely) reproductively isolated. The first group does not differ from Mus musculus L. The second exhibits several new biochemical traits and should probably be considered a different species.
- Published
- 1976
29. Evidence for the presence of two sympatric species of mice (genus Mus L.) in southern France based on biochemical genetics.
- Author
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Britton J and Thaler L
- Subjects
- Animals, Blood Proteins genetics, Esterases genetics, France, Gene Frequency, Genetics, Population, Isoenzymes, L-Lactate Dehydrogenase genetics, Mice classification, Oxidoreductases genetics, Species Specificity, Transaminases genetics, Mice genetics
- Abstract
Populations of mice established outdoors as well as indoors have been investigated at 24 loci using starch gel electrophoresis. Two reproductively isolated groups are recognized, one of which is referable to a house mouse subspecies, Mus musculus brevirostris, and the other to a different species. Mus spretus, contrary to the view of Schwarz and Schwarz that only one species of Mus is present in the Mediterranean Basin. The genetic distance between these two groups is larger than between any pair of investigated subspecies of M. musculus. M. m. brevirostris is biochemically almost indistinguishable from M. m. domesticus. On the other hand, M. spretus exhibits several allelic variants unknown or at most very infrequent in M. musculus, as for instance at the lactate dehydrogenase B-chain locus.
- Published
- 1978
- Full Text
- View/download PDF
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