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Background: Measurement of cross‐sectional muscle area (CSMA) at the mid third lumbar vertebra (L3) level from computed tomography (CT) images is becoming one of the reference methods for sarcopenia diagnosis. However, manual skeletal muscle segmentation is tedious and is thus restricted to research. Automated solutions are required for use in clinical practice. Purpose: The aim of this study was to compare the reliability of two automated solutions for the measurement of CSMA. Methods: We conducted a retrospective analysis of CT images in our hospital database. We included consecutive individuals hospitalized at the Grenoble University Hospital in France between January and May 2018 with abdominal CT images and sagittal reconstruction. We used two types of software to automatically segment skeletal muscle: ABACS, a module of the SliceOmatic software solution "ABACS‐SliceOmatic," and a deep learning‐based solution called "AutoMATiCA." Manual segmentation was performed by a medical expert to generate reference data using "SliceOmatic." The Dice similarity coefficient (DSC) was used to measure overlap between the results of the manual and the automated segmentations. The DSC value for each method was compared with the Mann–Whitney U test. Results: A total of 676 hospitalized individuals was retrospectively included (365 males [53.8%] and 312 females [46.2%]). The median DSC for SliceOmatic vs AutoMATiCA (0.969 [5th percentile: 0.909]) was greater than the median DSC for SliceOmatic vs. ABACS‐SliceOmatic (0.949 [5th percentile: 0.836]) (p < 0.001). Conclusions: AutoMATiCA, which used artificial intelligence, was more reliable than ABACS‐SliceOmatic for skeletal muscle segmentation at the L3 level in a cohort of hospitalized individuals. The next step is to develop and validate a neural network that can identify L3 slices, which is currently a fastidious process. [ABSTRACT FROM AUTHOR]

Previous studies have inconsistently reported associations between refractory ceramic fibers (RCFs) or mineral wool fibers (MWFs) and the presence of pleural plaques. All these studies were based on chest radiographs, known to be associated with a poor sensitivity for the diagnosis of pleural plaques. Does the risk of pleural plaques increase with cumulative exposure to RCFs, MWFs, and silica? If the risk does increase, do these dose-response relationships depend on the co-exposure to asbestos or, conversely, are the dose-response relationships for asbestos modified by co-exposure to RCFs, MWFs, and silica? Volunteer workers were invited to participate in a CT scan screening program for asbestos-related diseases in France. Asbestos exposure was assessed by industrial hygienists, and exposure to RCFs, MWFs, and silica was determined by using job-exposure matrices. A cumulative exposure index (CEI) was then calculated for each subject and separately for each of the four mineral particle exposures. All available CT scans were submitted to randomized double reading by a panel of radiologists. In this cohort of 5,457 subjects, significant dose-response relationships were determined after adjustment for asbestos exposure between CEI to RCF or MWF and the risk of PPs (ORs of 1.29 [95% CI, 1.00-1.67] and 1.84 [95% CI, 1.49-2.27] for the highest CEI quartile, respectively). Significant interactions were found between asbestos on one hand and MWF or RCF on the other. This study suggests the existence of a significant association between exposure to RCFs and MWFs and the presence of pleural plaques in a large population previously exposed to asbestos and screened by using CT scans. [ABSTRACT FROM AUTHOR]

Background: The impact of anterior cruciate ligament reconstruction (ACLR) on the performance and career of professional soccer players has not been extensively investigated. Purpose: To evaluate in professional European soccer players (1) the ACL injury incidence, (2) the return-to-play (RTP) rate and time after ACLR, (3) career survival and athlete performance in the first 3 postoperative seasons after RTP, (4) factors likely related to different outcomes after ACLR, and (5) any related differences between the top 8 European soccer leagues. Study Design: Descriptive epidemiology study. Methods: Included were professional soccer players in the top 8 European Soccer leagues (Serie A [Italy], Premier League [England], Ligue 1 [France], LaLiga [Spain], Bundesliga [Germany], Jupiler Pro League [Belgium], Liga NOS [Portugal], and Premier Liga [Russia]) who sustained an ACL injury during seasons 2014 to 2015, 2015 to 2016, and 2016 to 2017. Data were retrieved from publicly available online sources. Outcomes were evaluated based on player age (<25 years, 25-30 years, and >30 years), position (goalkeeper, defender, midfielder, and forward), affected side (dominant vs nondominant), and league. Results: Overall, 195 players sustained an ACL injury, for a mean annual ACL injury incidence of 1.42%. The RTP rate was 95%, with a mean RTP time of 248 ± 136 days. Within the third postoperative season, 66 players (36%) competed in a lower level national league, and 25 (13.6%) ended their careers; a significant reduction in the mean minutes played per season was found in all 3 postoperative seasons. Player age correlated significantly with reduction in performance or recovery from an ACL injury. No significant correlation was found between postoperative player performance and affected side, position, league, or time to RTP. Conclusion: A substantial ACL injury incidence was found in top European elite soccer players; however, a high RTP rate in a reasonable time was seen after ACLR. Nevertheless, professional soccer players experienced a short-term decline in their performance. [ABSTRACT FROM AUTHOR]

Man has always used writing to be able to communicate, express, and disseminate his thoughts. In time, many different coloured extracts of plant and animal origin have been used to produce inks; after the development of synthetic chemistry, artificial and synthetic dyes started to be widely exploited. The end of the 19th century marked great technological and industrial innovations in commercial production of artists' materials. To reveal ink formulations and build a database of red inks by different producers, we developed a multi-analytical approach and investigated a collection of writing inks produced in France in the late 19th – early 20th century. The materials used as binders, additives, dyes, and pigments have been investigated by high performance liquid chromatography coupled with diode array and tandem mass spectrometric detectors (HPLC-DAD-MS2), in situ derivatisation pyrolysis coupled with gas chromatography-mass spectrometry (Py(HMDS)-GC/MS), surface enhanced Raman (SERS, TLC-SERS) and X-Ray Fluorescence spectroscopies. Several dyes and pigments were detected, showing that the French ink's formulations of the early days of synthetic dye industry were based on rhodamine B and 6G , eosin Y , rose Bengal and methyl or crystal violet. Instead, as binder and additives only gum Arabic and shellac resin have been identified, respectively. Mass spectrometry also allowed us to detect possible by-products of the synthesis of ink's dyes and even early degradation products, that can be used for ink identification in historical writings and drawings. Our studies can pave the way to investigate inks in historical samples by introducing ultra-sensitive chromatographic and mass spectrometric methods in the array of analytical tools available to the chemist. [Display omitted] • We applied a multianalytical method to the analysis of historical red inks. • We applied Raman spectroscopy and TLC-SERS to study mixtures of organic pigments. • We investigated secondary components by HPLC-DAD-MS2. • We characterized binders and additives by Py(HMDS)-GC/MS. • We revealed a trend in French ink formulations from late 19th – early 20th cent. [ABSTRACT FROM AUTHOR]

This paper addresses the works of Henri Coudreau, a little-known French explorer of Guiana and Amazonia who was later forgotten by the 'heroic' histories of exploration because of his unruliness and nonconformist attitudes. Drawing on the literature of postcolonialism and tropicality as well as on recent studies of anti-colonialist geographies, I address for the first time Coudreau's geography from the perspective of anarchist and critical thinking. My main argument is that Coudreau's work is a further example of the complexity and heterogeneity of the European intellectual field during the imperial age. Despite having come of age intellectually among all the European racist and ethnocentric prejudices of his day, Coudreau developed a different outlook thanks to two factors, viz., his personal experience in living for years with the indigenous communities of Amazonia, and his exposure to anarchist anti-colonialist ideas through his collaboration with Elisée Reclus. Coudreau's tropical utopia of an independent Amazonia, and his endorsement of the stateless nature of local communities, ran counter to French imperial politics, occasioning Coudreau's dismissal from the French administration and his professional exile in Brazil at the time of the Franco-Brazilian border dispute (1897−1900). [ABSTRACT FROM AUTHOR]

This article addresses the life and works of a virtually unknown lady explorer, Octavie Renard-Coudreau (1867–1938), who continued an Amazonian exploration ‘alone’ after the death of her husband, the maverick French geographer Henri Coudreau (1859–1899). It extends and connects two main bodies of scholarship, the first on women travellers and feminist historical geographies, the second on scientific couples and collaborative partnerships in the history of sciences. I argue that, in addition to textual analysis, the social, biographical, cultural and political contexts of these travels allow a better understanding of the ambivalences that characterized Western travellers and scholars, both men and women, in imperial contexts. This helps avoiding essentialism and recovering the experiences of marginalized figures in the history of geography. The story of Octavie Coudreau has elements of originality that can stimulate new reflections on these points, also because her travel experience was not in the hegemonic Anglophone context, and she was acquainted with unorthodox and dissident geographers of that time. This article also contributes to studies on the influence which anarchist geographers such as Elisée Reclus, a supporter of the Coudreaus, exerted on explorers, though Octavie seemed to keep her distance from this former socialist inspiration. [ABSTRACT FROM AUTHOR]

Women were part of the political and scientific networks that contributed to Elisée Reclus’ enterprise of the New Universal Geography and built the “Antiauthoritarian International,” although they have been generally neglected by historiography. During the French Second Empire (1852-1870), Elie and Elisée Reclus collaborated with some of the most famous French militant women and feminists, like Louise Michel, Léodile Champseix (known under the masculine pseudonym of André Léo) and Noémi Reclus, including creating and participating in a league for women’s rights. This paper aims to clarify the working of these networks and their specific intersections with geography in the period of the 1871 Paris Commune and in the following ten years of exile, mainly through an analysis of correspondence by Louise Michel, Léodile Champseix and the members (male and female) of the Reclus family. My main hypothesis is that the collaboration between feminist militants and anarchist geographers, questioning patriarchy, endorsing ‘free union’ and mixed education, anticipated several features of successive anarchist feminisms, and that its study can be a useful contribution to a “Feminist Historical Geography.” [ABSTRACT FROM AUTHOR]

Hepatitis E virus (HEV) has emerged during the past decade as a causative agent of autochthonous hepatitis and is a clinical concern in Western developed countries. It has been increasingly recognized that pigs are a major reservoir of HEV of genotypes 3 and 4 worldwide and pig-derived food items represent a potential source of infections by these viruses in humans. Hepatitis E virus RNA testing was performed here on faeces from rectal swabs sampled in 2012 from 50 3-month-old farm pigs from the same farm located in south-eastern France than in a previous work conducted in 2007. Pig HEV sequences corresponding to genomic fragments of ORF2 and ORF1 genes were obtained after RT-PCR amplification with in-house protocols. Hepatitis E virus genotype was determined by phylogenetic analysis. Prevalence was similar to that determined 5 years earlier (68% versus 62%). Two robust phylogenetic clusters of HEV subtypes 3a and 3f were identified, and these sequences obtained in 2012 largely differ compared with those obtained in 2007. Notably, HEV sequences obtained in 2012 from a majority (62%) of the infected pigs belonged to subtype 3a, which was not previously described in France, including not being found in any of humans, pigs or wild boars. Further studies are needed to assess the circulation of HEV-3a in pigs and humans in this country. In addition, along with previous findings, this study supports the need for increased information to the public on the risk of HEV infection through contacts with pigs or consumption of pig-derived products in France. [ABSTRACT FROM AUTHOR]

Purpose: To understand why the false channel (FC) remains patent after surgery of type A acute aortic dissection (TAAAD). Materials and Methods: Postoperative contrast-enhanced computed tomography scans of 129 patients operated for TAAAD were analyzed, and a color-Doppler ultrasound examination (CDUS) of the supra-aortic vessels (SAVs) was performed in 12 patients. Results: The FC remained patent in 107 (82.9%) patients. The entry site was situated near the distal anastomosis in 43 (40.2%) patients and far from it in 44 (41.1%) patients. In 10 (9.35%) patients, an entry site was observed only in the SAVs. In 10 (9.35%) patients, no entry site was seen. Of the 12 patients explored with CDUS, a retrograde filling of the FC was observed in 11 patients. Conclusion: The frequent postoperative circulating aortic FC can be explained by the persistence of the primary entry tear, the presence of iatrogenic tears, and/or a retrograde filling in the SAVs. [ABSTRACT FROM AUTHOR]

Highlights: [•] Meta-analysis of data from 102 plots revealed an increase in defoliation in France. [•] Defoliation was mainly related to current and previous years precipitation, with little effect of method/observer bias. [•] Diverging and/or unexpected responses were detected even within the same species. [•] Quercus petraea plots were found to be sensitive to precipitation deficit. [•] This may question favouring Q. petraea (a common recommendation in France) to adapt forests to climate change. [Copyright &y& Elsevier]

Objectives: The objective was to compare the clinical results of ACL and ALL repair with those of standard ACL reconstruction + LET. The hypothesis was that combined repair is non-inferior to standard reconstruction + LET in adults with acute anterolateral knee instability. Methods: Patients with severe acute anterolateral instability (ACL + ALL injuries) who underwent early ACL surgery (injuryto-surgery interval < 14 days), a total of 58 patients, were prospectively enrolled in the study. ACL injuries were classified according to the Sherman classification. Patients with a proximal injury (types 1 and 2) were assigned to receive ACL repair (ACLrep) (n = 37), patients with types 3 and 4 and moderate tissue quality (n = 21) were assigned to receive ACL reconstruction (ACLrec) + lateral extra-articular tenodesis (LET). Repair consisted of transosseous suture of the stump to the anatomic origin of the ACL and direct repair of the ALL. Reconstruction consisted of standard ACL reconstruction by semitendinosus-gracilis (STG) autograft + LET. PROMs included IKDC, Lysholm, KOOS, and assessment of anteroposterior (AP) knee laxity in 2 years. Results: A sample size calculation showed that 21 patients in each group were needed for a 95% power (with a 95% CI) to show non-inferiority of the repair group considering an arthrometric side-to-side difference of at least +1.5. Patients in the ACLrep group showed better results on all scales: mean IKDC was 95.51 ± 52.4 versus 86.92 ± 13.92 (p = 0.02); mean KOOS was 97.49 ± 2.71 versus 93.50 ± 7.05 (p = 0.009); and mean Lysholm was 96.98 ± 5.14 versus 91.69 ± 10.08 (p = 0.02). There was no difference in the KT1000 measurement (ACLrep: 1.70 ± 1.02; ACLrec: 2.21 ± 0.80; p = 0.11). The one-sided 95% CI was 1.7 (to 2.02) (< at the 3.01 limit for non-inferiority) for ACLrep and 2.21 (to 2.46) for ACLrec, p = 0.147. Therefore, non-inferiority was proven. Conclusion: In acute knee instability resulting from a combined ACL and ALL injury, direct repair is non-inferior in terms of PROM and knee laxity compared to standard ACL + LET reconstruction in 2 years. [ABSTRACT FROM AUTHOR]

ALICE at the CERN LHC will investigate the physics of strongly interacting matter at extreme energy densities where the formation of the Quark Gluon Plasma is expected. Its properties can be studied from observations like the production of mesons with charm and beauty quarks. These signals have to be studied as a function of energy density, which is determined by the centrality of collisions. One of the physics observables that is closely related with the centrality of the collision is the number of spectator nucleons that can be measured by the Zero Degree Calorimeters (ZDC). Having a direct geometric interpretation allows to extract the impact parameter with minimal model assumptions. This paper describes the readout system of the ZDC. The ZDC readout consists of a VME system with a ZDC Readout Card, a VME Processor, Discriminators, a ZDC Trigger Card, scalers, QDCs and TDCs. The system was successfully tested during the 2009 ALICE data taking and is currently operational at the LHC. [ABSTRACT FROM PUBLISHER]

The article offers information on the conodont genus Teridontus from the early Ordovican of Montagne Noire, France. It states that the conodont genus Teridontus was introduced in 1980 and it was based on the Late Cambrian species Oneotodus nakamurai Nogami. It also mentioned that Teridontus was later reported from either the Upper Cambrian or Lower Ordovician sediments in various locations around the world.

Two species of a new Tremadocian (Early Ordovician) conodont genus from the Saint Chinian Formation of the southern Montagne Noire, France, are erected: Hammannodus sarae gen. et sp. nov. and Hammannodus juliae gen. et sp. nov. They were found within a single storm-induced limestone nodule interbedded with offshore shales belonging to the regional Shumardia ( C.) pusilla (trilobite) Biozone, and to the Paltodus deltifer deltifer (conodont) Subzone. This conodont record is associated with the episodic development of carbonate productivity in temperate waters of the Montagne Noire platform, a process absent in neighbouring platforms of north-west Gondwana. The apparatus is composed of five coniform pyramidal elements occupying P and S positions and one bicostate element in the M position, having three or two sharp costae, respectively, with a subtriangular basal outline. [ABSTRACT FROM AUTHOR]

Ground stability is a major concern for land use planning and both natural and anthropogenic risk assessment, especially in urbanized areas. Space-borne differential radar interferometry provides a unique tool able to give a synoptic view of ground deformation with centimetric to millimetric vertical precision. Approaches for combining a wide range of radar images such as the permanent scatterers (PS) technique allow the estimation of the deformation history of single buildings. The PS approach has been exploited to investigate a test site particularly exposed to ground deformation hazards, namely the iron mining basin in Lorraine (France). In this Letter, a specific focus was set on the case of Roncourt, where precursor signs of a collapse affecting an area of ∼300 × 300 m2 have been identified. [ABSTRACT FROM AUTHOR]

The aim of this study was to compare helical CT with fiberoptic bronchoscopy findings to appraise the medium-term results of proximal-airways stenting. Twenty-five patients with 28 endobronchial metallic stents inserted for local advanced malignancy ( n=13) or benign diseases ( n=12) underwent follow-up CT from 3 days to 50 months (mean 8 months). All studies were obtained using helical CT with subsequent multiplanar reformation and three-dimensional reconstruction including virtual bronchoscopy. The location, shape, and patency of stents and adjacent airway were assessed. The results of CT were compared with the results of fiberoptic bronchoscopy obtained with a mean delay of 2.5 days (SD 9 days) after CT scan. Twelve stents (43%) remained in their original position, patent and without deformity. Sixteen stents were associated with local complications: migration ( n=6); external compression with persistent stenosis ( n=4); local recurrence of malignancy ( n=4); fracture ( n=1); and non-congruence between the airway and the stent ( n=1). The CT demonstrated all the significant abnormalities demonstrated at fiberoptic bronchoscopy except two moderate stenoses (20%) related to granulomata at the origin of the stent. Ten of 14 stents inserted for benign conditions were without complications as compared with 2 of 14 in malignant conditions ( p=0.008). Computed tomography is an accurate noninvasive method for evaluating endobronchial stents. The CT is a useful technique for follow-up of patients who have undergone endobronchial stenting. [ABSTRACT FROM AUTHOR]

[Display omitted] • Growth was negatively related to defoliation in conifers and broadleaves. • Even slightly defoliated trees (15%) showed significant growth reduction. • Overall, growth decreased by 0.7–0.9% per unit increase of defoliation. • Defoliation shifts can point at changes in growth and carbon sequestration. Climate and weather fluctuations and changes are the most important environmental drivers of tree canopy defoliation, an indicator of forest health. We examined the relationship between tree defoliation and Basal Area Increment (BAI), a dimension of tree growth related to wood biomass increment and carbon sequestration and therefore to the climate change mitigation potential of forests. We analysed data from mostly even-aged, single-species permanent monitoring plots in France over two growing periods (1995–2004: 47 plots, 2008 trees; 2000–2009: 63 plots, 3116 trees) and for which precipitation deficit was identified as the main environmental driver of defoliation. Trees from ten different species were assessed annually for defoliation and measured periodically for growth, from which we derived periodical (10-year) BAI (BAI period). We investigated (i) direction and significance of defoliation-BAI period relationships and (ii) occurrence, size and significance of BAI deviation of progressively defoliated trees in proportion to the BAI of undefoliated trees (BAI rel). Analyses were first carried out at the level of individual plots, with results subsequently evaluated using meta-analysis and further aggregated at different levels (all species, functional groups, individual species). BAI period resulted negatively and significantly related to defoliation, with a significant reduction detected already at slight (15%) defoliation level. A generalized statistically significant reduction of BAI rel was obvious, leading to an estimated reduction of 0.7–0.8% per 1% increase in defoliation for conifers and 0.9% for broadleaves. Considering the observed distribution of trees along the defoliation range, our results indicate an overall growth reduction of ca. 42% in comparison to a theoretical population of undefoliated trees. Shifts in such a distribution can result into loss or gain of growth, which in turn may have cascading effects on carbon sequestration and therefore on land-climate interactions. In the context of the significant increase in defoliation observed in Europe in recent decades, our results suggest that even slight and moderate variations in defoliation may have had a significant impact on tree and forest growth. [ABSTRACT FROM AUTHOR]

Objective: the aim of this study was to evaluate the association between interstitial lung abnormalities, asbestos exposure and age in a population of retired workers previously occupationally exposed to asbestos. Methods: previously occupationally exposed former workers to asbestos eligible for a survey conducted between 2003 and 2005 in four regions of France, underwent chest CT examinations and pulmonary function testing. Industrial hygienists evaluated asbestos exposure and calculated for each subject a cumulative exposure index (CEI) to asbestos. Smoking status information was also collected in this second round of screening. Expert radiologists performed blinded independent double reading of chest CT-scans and classified interstitial lung abnormalities into: no abnormality, minor interstitial findings, interstitial findings inconsistent with UIP, possible or definite UIP. In addition, emphysema was assessed visually (none, minor: emphysema <25%, moderate: between 25 and 50% and severe: >50% of the lung). Logistic regression models adjusted for age and smoking were used to assess the relationship between interstitial lung abnormalities and occupational asbestos exposure. Results: the study population consisted of 2157 male subjects. Interstitial lung abnormalities were present in 365 (16.7%) and emphysema in 444 (20.4%). Significant positive association was found between definite or possible UIP pattern and age (OR adjusted =1.08 (95% CI: 1.02–1.13)). No association was found between interstitial abnormalities and CEI or the level of asbestos exposure. Conclusion: presence of interstitial abnormalities at HRCT was associated to aging but not to cumulative exposure index in this cohort of former workers previously occupationally exposed to asbestos. [ABSTRACT FROM AUTHOR]

Background: The purpose of this study was to test the diagnostic performance of clinical judgment for the prediction of a significant injury with whole-body computed tomography scanning after high-energy trauma.Methods: The authors conducted an observational prospective study in a single level-I trauma center. Adult patients were included if they had an isolated high-energy injury. Senior trauma leaders were asked to make a clinical judgment regarding the likelihood of a significant injury before performance of a whole-body computed tomography scan. Clinical judgments were recorded using a probability diagnosis scale. The primary endpoint was the diagnosis of a serious-to-critical lesion on the whole-body computed tomography scan. Diagnostic performance was assessed using receiver operating characteristic analysis.Results: Of the 354 included patients, 127 patients (36%) had at least one injury classified as abbreviated injury score greater than or equal to 3. The area under the receiver operating characteristic curve of the clinical judgment to predict a serious-to-critical lesion was 0.70 (95% CI, 0.64 to 0.75%). The sensitivity of the clinical judgment was 82% (95% CI, 74 to 88%), and the specificity was 49% (95% CI, 42 to 55%). No patient with a strict negative clinical examination had a severe lesion (n = 19 patients). The sensitivity of the clinical examination was 100% (95% CI, 97 to 100%) and its specificity was 8% (95% CI, 5 to 13%).Conclusions: Clinical judgment alone is not sufficient to reduce whole-body computed tomography scan use. In patients with a strictly normal physical examination, whole-body computed tomography scanning might be avoided, but this result deserves additional study in larger and more diverse populations of trauma patients. [ABSTRACT FROM AUTHOR]

Abstract: The ATLAS Muon Spectrometer is designed to measure the momentum of muons with a resolution of dp/p=3% at 100GeV and 10% at 1TeV. For this task, the spectrometer employs 355,000 Monitored Drift Tubes (MDTs) arrayed in 1200 chambers. Calibration (RT) functions convert drift time measurements into tube-centered impact parameters for track segment reconstruction. RT functions depend on MDT environmental parameters and so must be appropriately calibrated for local chamber conditions. We report on the creation and application of a gas monitor system based calibration program for muon track reconstruction in the LHC startup phase. [Copyright &y& Elsevier]

Abstract: ALICE (a large ion collider experiment) is the LHC experiment dedicated to the study of ultra-relativistic heavy ion collisions. The ALICE muon spectrometer covers a large range in pseudo-rapidity and is designed to study quarkonia and heavy flavours decaying into (di-)muons. The high particle multiplicities environment in such collisions require a specific, fast and efficient trigger system, the muon trigger. It consists of four planes of RPC detectors, covering an area of 36m2 each, 21k front-end channels and a fast-decision electronics. The muon trigger is designed to reconstruct (muon) tracks and deliver a trigger signal each 25ns (40MHz) with a total latency of 800ns. The hit position on the RPC is measured in two orthogonal directions with an accuracy of about 1cm. The performance measured with the first p–p collisions at carried out in December 2009 is reported. [Copyright &y& Elsevier]

Hepatitis E virus (HEV) is a leading cause of waterborne acute hepatitis in developing countries. In Europe, HEV causes a zoonotic disease and is hyperendemic in southern France. Four HEV genotypes (1 to 4) have been defined, and the most used classification divides them into 24 subtypes. Autochthonous European HEV strains belong in majority to genotype 3. Subtypes 3c, 3f, and 3e are representative of the HEV diversity in France. HEV causes chronic hepatitis in solid-organ transplant recipients in Europe, and viral characteristics associated with chronicity are poorly documented. We sequenced 343-nucleotide-long HEV genomic fragments from the serum of eight chronically infected kidney transplant recipients and a near-full-length genome in one case. We identified in four patients (50%) HEV of subtype 3i, not described previously in France. If shorter genomic fragments were used in phylogenetic analyses, these HEV sequences were clustered with open reading frame 2 (ORF2) fragments labeled as subtype 3c. At least five of the eight HEV 3i sequences recovered from humans in our phylogenetic analyses were from chronically infected kidney transplant recipients. These data show that the description of the prevalence and geographical distribution of HEV subtypes may be partially inaccurate and that criteria for classification as 3i and 3c should be clarified. Extended molecular virology analyses are required to improve knowledge of HEV epidemiology and determinants of chronic HEV infection., (Copyright © 2014, American Society for Microbiology. All Rights Reserved.)

In Europe, autochthonous hepatitis E is caused by genotype 3 hepatitis E virus (HEV) in almost all cases. A total of 15 infections with genotype 4 HEV were diagnosed in France from May 2009 to April 2012, and all but one of the HEV-4 strains implicated in these infections were genetically related and highly similar to HEV-4 sequences isolated from swine in Belgium. In addition, 5 autochthonous HEV-4 infections have been described in the region of Lazio, Italy, during March and April 2011, and these HEV sequences were 100% identical to one another but showed relatively low similarity (74-85%) to HEV-4 RNA samples collected in France. We report 6 additional HEV-4 infections that were diagnosed from May to July 2012 which represented 50% of the HEV infections diagnosed during this period in our clinical microbiology laboratory. Five of these HEV-4 strains were associated with autochthonous infections and were clustered together and with the majority of HEV-4 previously described in France, whereas the sixth strain was genetically divergent. Taken together with reports from other teams, these observations indicate that autochthonous infections with HEV-4 are emerging in Europe and have been transmitted by at least two distinct sources., (Copyright © 2013 S. Karger AG, Basel.)