1. In vitro exposure to triazoles used as fungicides impairs human granulosa cells steroidogenesis.
- Author
-
Serra, Loïse, Estienne, Anthony, Caria, Giovanni, Ramé, Christelle, Jolivet, Claudy, Froger, Claire, Henriot, Abel, Amalric, Laurence, Guérif, Fabrice, Froment, Pascal, and Dupont, Joëlle
- Subjects
- *
GRANULOSA cells , *TRIAZOLES , *ARYL hydrocarbon receptors , *FUNGICIDES , *GROUNDWATER , *STAR-branched polymers - Abstract
Triazoles are the main components of fungicides used in conventional agriculture. Some data suggests that they may be endocrine disruptors. Here, we found five triazoles, prothioconazole, metconazole, difenoconazole, tetraconazole, and cyproconazole, in soil or water from the Centre-Val de Loire region of France. We then studied their effects from 0.001 µM to 1000 µM for 48 h on the steroidogenesis and cytotoxicity of ovarian cells from patients in this region and the human granulosa line KGN. In addition, the expression of the aryl hydrocarbon receptor (AHR) nuclear receptor in KGN cells was studied. Overall, all triazoles reduced the secretion of progesterone, estradiol, or both at doses that were non-cytotoxic but higher than those found in the environment. This was mainly associated, depending on the triazole, with a decrease in the expression of CYP51, STAR, CYP11A1, CYP19A1, or HSD3B proteins, or a combination thereof, in hGCs and KGN cells and an increase in AHR in KGN cells. • Triazoles are found in the soil, surface and underground water in France. • Prothio-/Metco- and Difeno-conazole exert cytotoxic effects in human granulosa cells. • Prothio-/Metco- and Difeno-conazole decrease steroidogenesis at non-cytotoxic doses. • Prothio-/Metco- and Difeno-conazole alter ovarian cells at higher doses than environmental doses. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF