Fars province in the south of Iran was surveyed for Asilidae (Insecta: Diptera) during 2006-2007. Twenty six species, belonging to 19 genera and 7 subfamilies: Apocleinae (5 species), Asilinae (7 species), Dasypogoninae (2 species), Laphriinae (2 species), Laphystiinae (1 species), Leptogastrinae (1 species) and Stenopogoninae (8 species), were recorded. Of these, 5 species are new to the fauna of Iran. Additionally 2 subfamilies, 10 genera, and 22 species were recorded for the first time from Fars province. Distributional data for each species are included. [ABSTRACT FROM AUTHOR]