1. Restriction fragment length polymorphisms distinguish Leptospira borgpetersenii serovar hardjo type hardjo-bovis isolates from different geographical locations.
- Author
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Zuerner RL, Ellis WA, Bolin CA, and Montgomery JM
- Subjects
- Americas epidemiology, Animals, Blotting, Southern, Cattle, Cattle Diseases epidemiology, DNA Transposable Elements genetics, DNA, Bacterial genetics, DNA, Bacterial metabolism, Deoxyribonucleases, Type II Site-Specific metabolism, Europe epidemiology, Genome, Bacterial, Humans, Leptospira genetics, Leptospirosis epidemiology, Leptospirosis microbiology, Middle East epidemiology, New Zealand epidemiology, Nucleic Acid Hybridization, Plasmids genetics, Restriction Mapping, Swine, Cattle Diseases microbiology, Leptospira classification, Leptospirosis veterinary, Polymorphism, Restriction Fragment Length
- Abstract
Genetic variability among Leptospira borgpetersenii serovar hardjo type hardjo-bovis isolates representing several geographical regions was determined by restriction endonuclease analysis. Five previously unidentified EcoRI digestion patterns and one previously unidentified HhaI digestion pattern were seen with the various isolates. The copy number and genomic distribution of an L. borgpetersenii insertion sequence (IS1533) was determined. Hardjo-bovis isolate 033 (the type strain for hardjo-bovis) contained 40 well dispersed copies of IS1533. IS1533 probes were used to compare hardjo-bovis isolates by DNA blot hybridization analysis. Use of these probes showed the presence of additional genetic heterogeneity among hardjo-bovis isolates, which restriction endonuclease analysis did not show. Pulsed-field gel electrophoretic analysis of DNAs from several isolates suggested that some polymorphisms arose by genomic rearrangements. All hardjo-bovis isolates were categorized into 14 distinct groups on the basis of common hybridization and endonuclease digestion patterns. Most of these groups were isolated from distinct geographical regions, suggesting that several different clonal populations of hardjo-bovis exist.
- Published
- 1993
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