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Gene expression variation is pervasive across all levels of organismal organization, including development. Few studies, however, have examined variation in developmental transcriptional dynamics among populations, or how it contributes to phenotypic divergence. Indeed, the evolution of gene expression dynamics when both the evolutionary and temporal timescale are comparatively short remains relatively uncharacterized. Here, we examined coding and non-coding gene expression in the fat body of an ancestral African and a derived European Drosophila melanogaster population across three developmental stages spanning ten hours of larval development. Between populations, expression divergence was largely stage-specific. We detected higher expression variation during the late wandering stage, which may be a general feature of this stage. During this stage, we also detected higher and more extensive lncRNA expression in Europe, suggesting that lncRNA expression may be more important in derived populations. Interestingly, the temporal breadth of protein-coding and lncRNA expression became more restricted in the derived population. Taken together with the signatures of potential local adaptation that we detected at the sequence level in 9–25% of candidate genes (those showing evidence of expression divergence between populations), this finding suggests that gene expression becomes more developmental stage-specific during adaptation to new environments. We further used RNAi to identify several candidate genes that likely contribute to known phenotypic divergence between these populations. Our results shed light on the evolution and dynamics of expression variation over short developmental and evolutionary timescales, and how this variation contributes to population and phenotypic divergence. Author summary: Gene regulatory changes are thought to be important when a species adapts to a new environment because they can temporally and spatially fine-tune gene expression. In this study, we compared gene expression across three developmental stages between a population of the fruit fly Drosophila melanogaster from its center of origin in Africa and a derived population in Europe, which has adapted to this new environment. Gene expression differences between the populations were highly specific to each developmental stage. The expression of long non-coding RNA (lncRNA, a type of non-protein coding gene) was higher and more extensive during the last larval stage in Europe, suggesting an increased importance of lncRNA regulation in derived populations. Gene expression became more temporally restricted in the European population, which together with the potential signatures of adaptation we detected in candidate genes (those showing expression changes between populations), suggests that gene expression becomes more temporally-specific during adaptation to new environments. We also found that several candidate genes likely contribute to known trait differences between these populations. Our study helps us further understand the evolution of gene expression variation over short developmental and evolutionary timescales as well as how this variation influences phenotypic differences among individuals. [ABSTRACT FROM AUTHOR]

One of the most common hematological malignancies is multiple myeloma (MM) that its mortality and morbidity have increased. The incidence rate of MM is suggested to be higher in Europe and various kinds of therapeutic strategies including stem cell transplantation. However, MM treatment is still challenging and gene therapy has been shown to be promising. The non-coding RNAs (ncRNAs) including miRNAs, lncRNAs and circRNAs are considered as key players in initiation, development and progression of MM. In the present review, the role of ncRNAs in MM progression and drug resistance is highlighted to provide new insights for future experiments for their targeting and treatment of MM. The miRNAs affect proliferation and invasion of MM cells, and targeting tumor-promoting miRNAs can induce apoptosis and cell cycle arrest, and reduces proliferation of MM cells. Furthermore, miRNA regulation is of importance for modulating metastasis and chemotherapy response of tumor cells. The lncRNAs exert the same function and determine proliferation, migration and therapy response of MM cells. Notably, lncRNAs mainly target miRNAs in regulating MM progression. The circRNAs also target different molecular pathways in regulating MM malignancy that miRNAs are the most well-known ones. Furthermore, clinical application of ncRNAs in MM is discussed. [Display omitted] [ABSTRACT FROM AUTHOR]