1. The complete cDNA sequence encoding plasminogen from the European hedgehog (Erinaceus europaeus).
- Author
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Belczewski AR, Laplaud PM, Chapman MJ, and Koschinsky ML
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Blotting, Northern, Cloning, Molecular, DNA, Complementary genetics, DNA, Complementary isolation & purification, Europe, Glycosylation, Haplorhini, Humans, Mice, Molecular Sequence Data, Plasminogen chemistry, Plasminogen metabolism, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Hedgehogs genetics, Plasminogen genetics
- Abstract
Using a PCR-based strategy, we have determined the complete cDNA sequence encoding hedgehog plasminogen (Plg). The 2700-nucleotide cDNA (corresponding to a 2.9-kb liver-derived transcript) encodes an open reading frame of 811 amino acids which shares 74-76% identity with Plg characterized from mouse, human and rhesus monkey. Residues corresponding to the catalytic triad, tPA-cleavage site, as well as seven of the eight lysine-binding residues in kringle IV are conserved in the hedgehog. However, potential N-linked glycosylation sites which have been reported in human and rhesus Plg are not present in analogous positions in the hedgehog Plg sequence.
- Published
- 1996
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