1. European and Mexican vs US diagnostic extracts of Bermuda grass and cat in skin testing.
- Author
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Larenas-Linnemann D, Cruz AA, Gutierrez IR, Rodriguez P, Shah-Hosseini K, Michels A, and Mösges R
- Subjects
- Adult, Animals, Cats, Cynodon chemistry, Double-Blind Method, Europe, Female, Humans, Male, Mexico, Middle Aged, Rhinitis, Allergic, Perennial immunology, Rhinitis, Allergic, Seasonal immunology, Skin Tests adverse effects, Therapeutic Equivalency, United States, Young Adult, Allergens administration & dosage, Allergens adverse effects, Allergens immunology, Cynodon immunology, Rhinitis, Allergic, Perennial diagnosis, Rhinitis, Allergic, Seasonal diagnosis
- Abstract
Background: Laboratory testing of various diagnostic extracts has shown lower potencies for several European and Mexican extracts relative to the US Food and Drug Administration (FDA) reference (10,000 BAU/mL). Quantitative skin prick testing (QSPT) with Dermatophagoides pteronyssinus extracts have previously shown a similar picture., Objective: To compare European and Mexican Bermuda grass (BG) and cat diagnostic extracts against an FDA-validated extract using QSPT., Methods: Six diagnostic BG and cat extracts (1 reference FDA extract, 3 European extracts, 1 imported nonstandardized extract from the United States, and 1 Mexican extract) were tested with quadruplicate QSPT, as a concentrate and as 2 serial 2-fold dilutions, in cat and BG allergic individuals., Results: BG showed good dose response in wheal size for the concentrate (1:2-1:4 dilutions; steep part of the curve). Cat showed poorer dose response. The Wilcoxon test for linked random samples was used to investigate whether the distribution of the reference differed from each of the test extracts to a statistically significant degree (2-sided asymptotic significance, α = .05). All BG and 2 cat extracts were statistically less potent than the 10,000 BAU/mL US reference. European BG extracts were 7,700, 4,100, and 1,600 BAU/mL, and cat extracts were 12,500, 4,400, and 5,100 BAU/mL., Conclusions: The potency of some diagnostic extracts of BG and cat used in Europe, Mexico, and the United States differs, with the US extracts being generally more potent. On the basis of provocation tests, optimal diagnostic concentrations should be determined. Similar comparisons using other manufacturers and therapeutic extracts might be interesting., (Copyright © 2011 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2011
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