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1. [Glycine determination according to the European Pharmacopoeia].

Author

Peinhardt G

Subjects
Europe, Fluorescent Dyes analysis, Formaldehyde, Glyoxylates, Indicators and Reagents, Lactones analysis, Oxidation-Reduction, Pharmacopoeias as Topic, Reference Standards, Resorcinols, Glycine analysis
Abstract

The treatment of an aqueous solution of glycine with hypochlorite yields formaldehyde and glyoxylic acid. After acidification and removing formaldehyde by boiling glyoxylic acid reacts with resorcinol to the lactone of bis-2,4-dihydroxyphenyl acetic acid. Under alkaline conditions the lactone is oxidized to the violet oxonol anion and subsequently, after hydrolysis of the lactone, to the yellow fluorescent carboxylate anion.

Published
2004

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