1. Genotypic and phenotypic characterization of the first Latin America isolates of Corynebacterium rouxii, a recently described member of the Corynebacterium diphtheriae complex reported in Europe.
- Author
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de Oliveira Sant'Anna L, Dos Santos LS, Ramos JN, Bokermann S, Bernardes Sousa MÂ, Prates FD, Mattos-Guaraldi AL, Vieira VV, and Araújo MRB
- Subjects
- Animals, Corynebacterium diphtheriae genetics, Corynebacterium diphtheriae isolation & purification, Corynebacterium diphtheriae classification, Corynebacterium diphtheriae drug effects, Cats, Genotype, Latin America, Europe, Cat Diseases microbiology, Phenotype, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Diphtheria microbiology, DNA, Bacterial genetics, Corynebacterium genetics, Corynebacterium isolation & purification, Corynebacterium classification, Corynebacterium drug effects, Phylogeny, RNA, Ribosomal, 16S genetics, Corynebacterium Infections microbiology, Anti-Bacterial Agents pharmacology, Microbial Sensitivity Tests
- Abstract
The genus Corynebacterium is the largest genera among corynebacteria and has a range of species widely spread in ecological niches, some with epidemic potential and capable of causing fatal diseases. In recent years, due to the reclassifications and discoveries of new potentially toxin-producing species, microbiological identification and epidemiological control have been compromised, becoming possible only with sequencing techniques. Two bacterial strains isolated from a cat were identified by MALDI-TOF mass spectrometry as Corynebacterium diphtheriae and sent to the collaborating center of the Brazilian Ministry of Health for molecular identification and determination of toxigenicity potential, which were initially performed by multiplex PCR method. In addition, the antimicrobial susceptibility profile was determined according to BrCAST. Finally, for the final identification at the species level and effective epidemiological monitoring, the sequencing of the 16S rRNA and rpoB housekeeping genes was carried out. The isolates were identified as nontoxigenic C. diphtheriae strains by mPCR. Both strains were found susceptible to all antimicrobial agents. Although the identification at the species level was not possible through similarity analysis of S rRNA and rpoB housekeeping genes, the phylogenetic analysis showed that the isolates belonged to the species Corynebacterium rouxii with a high value of reliability. This is the first report of the isolation of C. rouxii in Latin America. Molecular identification, whether by the MALDI-TOF mass spectrometry or PCR techniques, does not discriminate C. rouxii from C. diphtheriae, requiring gene sequencing and phylogenetic analysis for correct identification at the species level., Competing Interests: Declarations. Ethical approval: The genetic heritage information of the samples used in this manuscript is registered in the National Genetic Heritage and Associated Traditional Knowledge Management System (SisGen) under registration number A31DF7E. Conflict of interest: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
- Published
- 2024
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