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The fruit pests Cydia pomonella (L.), Crapholita molesta (Busck), Grapholitafunebrana Treitschke, and Grapholita lobarzewskii Nowicki (all Lepidoptera: Tortricidae) are sympatric in Europe, and some of them are expanding their distribution range worldwide by continuous invasion into new areas. Identification of the small larvae by morphological characters and damage symptoms is difficult, and many misidentifications among these tortricids of the tribe Grapholitini are reported. In the current study, the species were separated using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analyses. Samples were obtained from different countries and continents, and the mitochondrial cytochrome oxidase subunit I (COl) fragments of one individual per species and geographical site were sequenced. Based on the alignments of the amplified COl sequences, the PCR-RFLP method was established with 42 individuals and successfully validated using a total of 122 individuals of the four species. Results document that this method allows for discrimination of larvae, pupae, and adult specimens of all four species and can be used for a rapid and reliable identification of C. pomonella, C. molesta, C. funebrana, and G. lobarzewskii. Moreover, it can easily be expanded to other internal tortricid fruit feeder species that occur on different continents. [ABSTRACT FROM AUTHOR]

Objective: Coronary artery disease (CAD) remains one of the major causes of death worldwide. Despite considerable advances in the prevention and treatment of CAD, its complications, morbidity and mortality still remain very high, and vary widely across different ethnic groups., Materials and Methods: To detect genes involved in the development of CAD, we collected gene expression studies in the blood samples of CAD patients from different continents by searching the Gene Expression Omnibus database (GEO), performed a comparative analysis of gene expression between CAD patients and normal controls (NC) in each continent and identified the common set of differentially expressed genes (DEGs) between CAD patients and NC across different continents. PPI networks of the common set of DEGs were established by Cytoscape software to understand their biological role in CAD., Results: A total of 575, 868 and 476 genes were identified to be significantly differentially expressed between CAD patients and NC in Asia, Europe and North America. 24 genes were found common in three different continents, and 6 genes were previously linked to CAD or atherosclerosis. In the PPIs network the significant hub proteins contained IRF4 (Degree = 23), PLAUR (Degree = 17) and HIST1H2AE (Degree = 15)., Conclusions: Not only did we detect gene expression differences in the blood samples between CAD and NC in Asia, Europe and North America population, but analysis of the three population groups revealed a common set of 24 genes regardless of differences related to race, ethnicity, lifestyle, and environmental factor which may provide key factors to understand the pathogenesis of CAD and lead to development of diagnostic markers and/or effective therapeutic strategies.