1. Nasosorption as a Minimally Invasive Sampling Procedure: Mucosal Viral Load and Inflammation in Primary RSV Bronchiolitis.
- Author
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Thwaites, Ryan S., Ito, Kazuhiro, Chingono, Jasmine M. S., Coates, Matthew, Jarvis, Hannah C., Tunstall, Tanushree, Anderson-Dring, Lauren, Cass, Lindsey, Rapeport, Garth, Openshaw, Peter J., Nadel, Simon, and Hansel, Trevor T.
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LAPAROSCOPIC surgery , *BRONCHIOLITIS , *INFANT diseases , *INTERFERONS , *INTERLEUKIN-10 , *ARTIFICIAL respiration , *BRONCHIOLE diseases , *CYTOKINES , *INFLAMMATION , *INTERLEUKINS , *NASAL mucosa , *RESPIRATORY syncytial virus , *VIRAL load , *CASE-control method , *RESPIRATORY syncytial virus infections , *DIAGNOSIS - Abstract
Background: Existing respiratory mucosal sampling methods are flawed, particularly in a pediatric bronchiolitis setting.Methods: Twenty-four infants with bronchiolitis were recruited: 12 were respiratory syncytial virus (RSV)-positive, 12 were RSV-negative. Infants were sampled by nasosorption and nasopharyngeal aspiration (NPA).Results: Nasosorption was well tolerated and identified all RSV+ samples. RSV load measured by nasosorption (but not NPA) correlated with length of hospital stay (P = .04) and requirement for mechanical ventilation (P = .03). Nasosorption (but not NPA) levels of interferon γ, interleukin 1β, CCL5/RANTES, and interleukin 10 (IL-10) were elevated in RSV+ bronchiolitis (all P < .05), furthermore CCL5 and IL-10 correlated with RSV load (P < .05).Conclusions: Nasosorption allowed measurement of RSV load and the mucosal inflammatory response in infants. [ABSTRACT FROM AUTHOR]- Published
- 2017
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